Team:Nevada/Notebook

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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Nevada_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Nevada_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Nevada | Team Example]]
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<!--- The Mission, Experiments --->
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!align="center"|[[Team:Nevada|Home]]
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<a href="https://2011.igem.org/Team:Nevada/Project">Project</a>
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Nevada Official Team Profile]
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<a href="https://2011.igem.org/Team:Nevada/Notebook">Notebook</a>
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!align="center"|[[Team:Nevada/Project|Project]]
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<a href="https://2011.igem.org/Team:Nevada/Parts">Parts</a>
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!align="center"|[[Team:Nevada/Parts|Parts Submitted to the Registry]]
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<a href="https://2011.igem.org/Team:Nevada/Media">Media</a>
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!align="center"|[[Team:Nevada/Modeling|Modeling]]
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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!align="center"|[[Team:Nevada/Notebook|Notebook]]
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!align="center"|[[Team:Nevada/Safety|Safety]]
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<a href="https://2011.igem.org/Team:Nevada/Foundraiser">Fundraisers</a>
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!align="center"|[[Team:Nevada/Attributions|Attributions]]
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<a href="http://www.igemteamnevada.webs.com">Official Site</a>
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<a href="https://2011.igem.org/Team:Nevada/Reference">Reference</a><br><br>
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<font color="red">E. Coli</font> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<font color="blue">Cyano</font> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<font color="green">Enzymology</font> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<font color="black">Media</font>
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<div id="weekly">
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Week 1 - June 1-4
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-----------------------
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E. Coli
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<br>
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Comment Here
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Cyano
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Comment Here
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==Notebook==
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=== [https://2011.igem.org/Team:Nevada/Notebook/EColiGroup <u>''E. coli'' group's notebook</u>] ===
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Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
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<ul>Rxn:
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  <li>1. glucose + ATP --Hx-> G-6-P + ADP</li>
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  <li>2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH</li>
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</ul>
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<br>
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Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
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Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations.
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Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.
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=== [https://2011.igem.org/Team:Nevada/Notebook/EnzymologyGroup <u>Enzymology group's notebook</u>] ===
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Media
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<a href="https://2011.igem.org/Team:Nevada/Notebook">Week 1</a>
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=== [https://2011.igem.org/Team:Nevada/Notebook/SynechocystisGroup <u>''Synechocystis'' group's notebook</u>] ===
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<a href="https://2011.igem.org/Team:Nevada/Notebook/June/Week2">Week 2</a>
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Latest revision as of 20:32, 30 June 2011


Week 1 - June 1-4
-----------------------
E. Coli
Comment Here
Cyano
Comment Here
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
    Rxn:
  • 1. glucose + ATP --Hx-> G-6-P + ADP
  • 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH

Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Week 1                                                                                                                                                         Week 2

Retrieved from "http://2011.igem.org/Team:Nevada/Notebook"