Team:Freiburg/Notebook/4 August
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{{:Team:Freiburg/Templates/header}} | {{:Team:Freiburg/Templates/header}} | ||
+ | <html> | ||
+ | <div id="notebook-page-header"> | ||
+ | <div id="notebook-back" width="100px" > | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/3_August">Previous entry</a> | ||
+ | </div> | ||
+ | <div id="notebook-title"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 4 August </a> | ||
+ | </div> | ||
+ | <div id="notebook-next"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/5_August">Next entry</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </html> | ||
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==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
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2µl Dpn1 <br/> | 2µl Dpn1 <br/> | ||
38µl DNA from quickchange (CcaR) <br/> | 38µl DNA from quickchange (CcaR) <br/> | ||
- | incubate at | + | incubate at 37°C for 1,5 h <br/> |
inactivate at 80°C for 20 min. <br/> | inactivate at 80°C for 20 min. <br/> | ||
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'''Investigator: Jakob''' | '''Investigator: Jakob''' | ||
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'''Documentation:''' | '''Documentation:''' | ||
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* (qc) CcaR in pSB1C3 (Cm resistance) | * (qc) CcaR in pSB1C3 (Cm resistance) | ||
* Stored in the fridge | * Stored in the fridge | ||
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*To-do: pick some colonies | *To-do: pick some colonies | ||
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===Testdigest of mutated CcaS=== | ===Testdigest of mutated CcaS=== | ||
'''Investigator: Julia''' | '''Investigator: Julia''' | ||
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10 μl total volume | 10 μl total volume | ||
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Give 3 μl of DNA in an eppi and add 7μl of the mastermix. | Give 3 μl of DNA in an eppi and add 7μl of the mastermix. | ||
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Incubate for about 1h at 37°C. | Incubate for about 1h at 37°C. | ||
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Add 1 μl Loading dye buffer and load the gel. | Add 1 μl Loading dye buffer and load the gel. | ||
- | + | Gelpicture: see red light receptor, lower lane.<br/> | |
+ | Samples C3e and C3c were send to GATC for sequencing. | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
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'''Investigators: Jakob''' | '''Investigators: Jakob''' | ||
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'''Documentation:''' | '''Documentation:''' | ||
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name samples: | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name samples: | ||
- | α β γ δ ε ζ with Kanamycin resistance in pSB1K3 | + | * α β γ δ ε ζ with Kanamycin resistance in pSB1K3 |
- | Lys + RBS with Chloramphenicol resistance pSB1C3 | + | * Lys + RBS with Chloramphenicol resistance pSB1C3 |
- | Lys complete with tetracycline resistance pSB1T3 | + | * Lys complete with tetracycline resistance pSB1T3 |
- | Stored: orange rack in the fridge | + | * Stored: orange rack in the fridge |
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Latest revision as of 01:05, 22 September 2011