Team:Paris Liliane Bettencourt/Notebook/2011/07/18/

From 2011.igem.org

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<p style="display:block;"><font color="red">Edward</font>
 
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</p><p style="display:block;"><u>Minipreps</u>
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{{:Team:Paris_Bettencourt/tpl_test}}
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</p>
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<ul><li>on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
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</li><li>on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
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</li><li>on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL
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<p style="display:block;"><font color="red">Edward</font>
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<p style="display:block;"><u>Restriction Digest of RBS-spoVg and T7 RNA polymerase </u>
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</p><p style="display:block;"><u>Overnight cultures (LB)</u>
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<ul><li>BBa_K145001 cut by  XbaI and PstI
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</li><li>BBa_K143021 cut by  SpeI and PstI
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<br />Final volume&nbsp;: 20 µL
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<u>DNA Gel electrophoresis (T7 RNA polymerase)</u>  
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<ul><li>1kb ladder
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</li><li>T7 undigested plasmid
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</li><li>Digested T7 (BBa_K145001 cut by  XbaI and PstI)
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</li></ul>
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<ul><li> BBa_I712074 (pT7),
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[[File:T7 electro 19072011.jpeg|thumb|center| ladder - non digested T7 plasmid - digested T7  ]]
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</li><li> BBa_K145001 (T7 RNA polymerase),
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[File:T7 electro 19072011 cut.jpeg|thumb|center| ladder - non digested T7 plasmid - digested T7  ]]
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</li><li> BBa_K143021 (RBS-spoVg colony 17)

Latest revision as of 16:03, 27 August 2011

Team IGEM Paris 2011