Team:Nevada/Notebook

From 2011.igem.org

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{{Nevada__Notebook_CSS}}
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<html xmlns="http://www.w3.org/1999/xhtml">
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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<head>
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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<meta http-equiv="content-type" content="text/html; charset=utf-8" />
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This is a template page. READ THESE INSTRUCTIONS.
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<meta name="description" content="" />
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<body>
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<div id="header">
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<div id="titleleft">
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<a href="https://2011.igem.org/Team:Nevada">Home</a>
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<a href="https://2011.igem.org/Team:Nevada/Project">Project</a>
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<a href="https://2011.igem.org/Team:Nevada/Notebook">Notebook</a>
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<a href="https://2011.igem.org/Team:Nevada/Parts">Parts</a>
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<a href="https://2011.igem.org/Team:Nevada/Media">Media</a>
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<a href="https://2011.igem.org/Team:Nevada/Foundraiser">Fundraisers</a>
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<a href="https://2011.igem.org/Team:Nevada/PhotoGallery">Gallery</a>
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<a href="http://www.igemteamnevada.webs.com">Official Site</a>
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<a href="https://2011.igem.org/Team:Nevada/Reference">Reference</a><br><br>
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<font color="red">E. Coli</font> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<font color="blue">Cyano</font> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<font color="green">Enzymology</font> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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<font color="black">Media</font>
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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<div id="weekly">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace.
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Week 1 - June 1-4
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<br>
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-----------------------
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</font>
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<br><font color="red">
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E. Coli
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<br>
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Comment Here
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<br><font color="blue">
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Cyano
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<br>
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Comment Here
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Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
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<br>
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<ul>Rxn:
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  <li>1. glucose + ATP --Hx-> G-6-P + ADP</li>
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  <li>2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH</li>
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</ul>
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<br>
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Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
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Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations.
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Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.
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{|align="justify"
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</font>
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Nevada_logo.png|200px|right|frame]]
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Media
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Nevada_team.png|right|frame|Your team picture]]
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<div id="bottom" align="center">
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|align="center"|[[Team:Nevada | Team Example]]
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<a href="https://2011.igem.org/Team:Nevada/Notebook">Week 1</a>
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<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Nevada|Home]]
 
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!align="center"|[[Team:Nevada/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Nevada Official Team Profile]
 
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!align="center"|[[Team:Nevada/Project|Project]]
 
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!align="center"|[[Team:Nevada/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Nevada/Modeling|Modeling]]
 
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!align="center"|[[Team:Nevada/Notebook|Notebook]]
 
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!align="center"|[[Team:Nevada/Safety|Safety]]
 
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!align="center"|[[Team:Nevada/Attributions|Attributions]]
 
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|}
 
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<a href="https://2011.igem.org/Team:Nevada/Notebook/June/Week2">Week 2</a>
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==Notebook==
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</div>
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
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</body>
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</html>

Latest revision as of 20:32, 30 June 2011


Week 1 - June 1-4
-----------------------
E. Coli
Comment Here
Cyano
Comment Here
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
    Rxn:
  • 1. glucose + ATP --Hx-> G-6-P + ADP
  • 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH

Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Week 1                                                                                                                                                         Week 2

Retrieved from "http://2011.igem.org/Team:Nevada/Notebook"