Team:Yale/Notebook/Week10

From 2011.igem.org

(Difference between revisions)
(Created page with "== Notebook: Week 5 == ''Friday'' - Planned/mapped out next 4 weeks - Took dialyzed bag with purified eGFP-RiAFP and divided it into 12 eppendorfs, containing 1 mL each. Flash-...")
 
(6 intermediate revisions not shown)
Line 1: Line 1:
-
== Notebook: Week 5 ==
+
{{:Team:Yale/Templates/Yale_Header_Else}}
-
 
+
<html>
-
''Friday''
+
<div id="text">
-
- Planned/mapped out next 4 weeks
+
      <div id="container">
-
- Took dialyzed bag with purified eGFP-RiAFP and divided it into 12 eppendorfs, containing 1 mL each. Flash-froze tubes in liquid nitrogen, then quickly used a hot edge to poke holes into top of eppendorfs to allow sublimation. Tubes then placed in lyophilizer for 3 nights.
+
<div id="left-col">
-
 
+
<ul id="nb">
-
''Saturday''
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week1">Week 1</a></li>
-
- Began cloning all pSB1AK8 constructs into pSB1C3 for parts submission (G3, H1, RiA, RiG, T2, Z2?):
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week2">Week 2</a></li>
-
- Digested 6 constructs and linearized pSB1C3 with EcoRI, PstI
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week3">Week 3</a></li>
-
- Also, PCR amplified eGFP segment from original eGFP-RiAFP plasmid to use as a control for survival assays
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week4">Week 4</a></li>
-
- Digested PCR fragment with eGFP with XbaI, PstI, and digested BBa_I712074 (strong T7 promoter) with SpeI and PstI
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week5">Week 5</a></li>
-
- Gel extracted all digested pSB1AK8 constructs using kit, gel visualized with SYBRSafe (digestion seemed successful)
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week6">Week 6</a></li>
-
 
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week7">Week 7</a></li>
-
''Sunday''
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week8">Week 8</a></li>
-
- Ligated gel-extracted pSB1AK8 fragments with pSB1C3; ligated eGFP PCR fragment with BBa_I712074
+
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week9">Week 9</a></li>
-
- Transformed ligations into DH5alpha cells, plated them
+
<li><a id="page" href="https://2011.igem.org/Team:Yale/Notebook/Week10">Week 10</a></li>
 +
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week11">Week 11</a></li>
 +
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week12">Week 12</a></li>
 +
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week13">Week 13</a></li>
 +
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week14">Week 14</a></li>
 +
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week15">Week 15</a></li>
 +
</ul>
 +
</div>
 +
<div id="entry">
 +
<h1>Week 10: July 17-24, 2011</h1>
 +
<ul>
 +
<li>Monday:<ul>
 +
<li>Western runny</li>
 +
<li>Cobalt purification His column of RiAFP</li>
 +
<li>survival assay, miniprep/cell cultures</li>
 +
</ul>
 +
Tuesday:<ul>
 +
<li>Farren meeting prep (transcriptomes, iwAFP paper)</li>
 +
<li>more protein purification, poster work</li>
 +
<li>Cell-lysate protein recrystallization</li>
 +
</ul></li><li>
 +
Wednesday:<ul>
 +
<li>Poster presentation</li>
 +
<li>SDS on purified fractions (image)</li>
 +
</ul></li><li>
 +
Thursday:<ul>
 +
<li>Successful purification</li>
 +
<li>More purification</li>
 +
</ul></li><li>
 +
Friday:<ul>
 +
<li>Purification of TmAFP, ZeAFP samples</li>
 +
</ul></li><li>
 +
Saturday:<ul>
 +
<li>Bulk purification of RiAFP</li>
 +
</ul></li><li>
 +
Sunday:<ul>
 +
<li>Try different Co purification and Ni purification</li>
 +
</ul></li>
 +
</ul>
 +
</div>
 +
<div style="clear:both"></div>
 +
</div>

Latest revision as of 02:28, 28 September 2011

iGEM Yale

Week 10: July 17-24, 2011

  • Monday:
    • Western runny
    • Cobalt purification His column of RiAFP
    • survival assay, miniprep/cell cultures
    Tuesday:
    • Farren meeting prep (transcriptomes, iwAFP paper)
    • more protein purification, poster work
    • Cell-lysate protein recrystallization
  • Wednesday:
    • Poster presentation
    • SDS on purified fractions (image)
  • Thursday:
    • Successful purification
    • More purification
  • Friday:
    • Purification of TmAFP, ZeAFP samples
  • Saturday:
    • Bulk purification of RiAFP
  • Sunday:
    • Try different Co purification and Ni purification

Retrieved from "http://2011.igem.org/Team:Yale/Notebook/Week10"