Team:Imperial College London/Project/Auxin/Notebook

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<h2>29th of July</h2>
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Our first transformations were a success! We have managed to transform our competent cell colonies with pSB1C3 containing BBa_K398500 and J23100 promoter to produce cell line 6. We also transformed the cells with part BBa_K316001 to produce cell line 7 and part BBa_K316005 to produce cell line 8. With these cell lines we will be able to make more copies of each part in preparation for the arrival of our synthesized sequences. We are under a tight schedule so efficiency is key.<br><br>
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Also, the cell line with the superfolded GFP integrated in its genome has been plated successfully. We have confirmed that these are the correct cell lines by looking at them under UV light. Their green glow was brighter than we expected. We hope to obtain some beautiful pictures for the root uptake experiment and the chemotaxis experiment.
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Latest revision as of 14:24, 2 August 2011