Thursday, July 28

From 2011.igem.org

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Latest revision as of 00:06, 29 July 2011

LABLED 6 CENTERFUGE TUBES

T3 R3 P3

T4 R4 P4

Removed 6 mutagenesis tubes from freezer labled as Mutagenesis P,R and T products grown up and spun down

Lysis by Alkali

The contents of these tubes was vortex-ed and poured into its corresponding centerfuge tube above each tube was rinsed with about 1 ml nuclease free water and poured into the corresponding centrifuge tubes.

These six centerfuge tubes were subsequently centerfuged at 4 degrees C. at 12,000 rpm for 30seconds twice. each tube was then decanted and wicked without disturbing the supernatant.

6 mLs of Solution I was added to each tube and vortex-ed to re-suspend the pellet.--Mduley 19:02, 28 July 2011 (CDT)

Team:Baltimore/Notebook

Hours

Revision as of 00:02, 29 July 2011 (view source) Mduley (Talk \| contribs) ← Older edit		Latest revision as of 00:06, 29 July 2011 (view source) TravisCR (Talk \| contribs)
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	6 mLs of Solution I was added to each tube and vortex-ed to re-suspend the pellet.--[[User:Mduley\|Mduley]] 19:02, 28 July 2011 (CDT)		6 mLs of Solution I was added to each tube and vortex-ed to re-suspend the pellet.--[[User:Mduley\|Mduley]] 19:02, 28 July 2011 (CDT)
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