Team:Freiburg/Notebook/27 August
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+ | <div id="notebook-page-header"> | ||
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+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/26_August">Previous entry</a> | ||
+ | </div> | ||
+ | <div id="notebook-title"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 27 August </a> | ||
+ | </div> | ||
+ | <div id="notebook-next"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/28_August">Next entry</a> | ||
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- | === | + | ==<span style="color:green;">green light receptor</span>== |
- | + | ===transformation with quickchanged CcaR=== | |
+ | '''Investigators:Julia''' | ||
+ | competent e.coli was transformed with 10 µl PCR reaction.<br/> | ||
+ | The CcaR is in a pSB1C3 vector.<br/> | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
- | === | + | ===Miniprep=== |
- | '''Investigators: | + | '''Investigators: Sophie''' |
+ | Miniprep of transformed cells with the blue light receptor. | ||
+ | To confirm the ligation of the inserts the samples were digested and loaded onto a gel. | ||
+ | The bands were not clear to see. | ||
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== |
Latest revision as of 01:12, 22 September 2011