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| - | <style type="text/css";>
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| - | .firstHeading{background-image: url(https://static.igem.org/mediawiki/2011/0/0a/Banniere_centrale_nucleaire.jpg);background-repeat: no repeat;}
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| - | <div id="language";>
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| - | <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/PresentationFr">Version Francaise</a>
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| - | <!-- Description projet -->
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| - | <div class="cadre" ; style="background-color:green; margin-left : 250px" >
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| - | <h1 style="color: white;"> A project anchored at the heart of current concerns </h1>
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| - | <p class="contenu">
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| - | <span style="line-height : 2em">
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| - | The activity of modern nuclear power plants with pressurized water reactors generates
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| - | radioactive effluents that contain among other things radioactive cobalt. The tubing of
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| - | the cooling circuit is made of a steel alloy rich in stable cobalt (59Co). Undergoing neutron
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| - | bombardment coming from the reactor, this stable cobalt changes into its radioactive isotope,
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| - | cobalt 60 (60Co).
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| - | The capture of this metal is interesting on a sanitary point of view, because it represents
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| - | a danger under both its radioactive and stable forms (carcinogenic). It also represents an
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| - | advantage on an environmental point of view, in order to avoid contamination of waters, soil
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| - | and groundwater. Even with a short half life, cobalt 60 emits high intensity gamma rays, and
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| - | decays to nickel, which is stable but polluting.
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| - | Controlled immobilization of radioactive cobalt is both an important sanitary and environmental
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| - | issue, which we intend to solve with an innovative and economical response. A researcher
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| - | from the Lyon INSA-ENS team, Agnès Rodrigues, has recently constructed a E.coli strain able
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| - | to eliminate 85% of radioactive cobalt (60Co), initially present as traces in a simulated nuclear
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| - | effluent made up of a mix of heavy metals, in only twice one-hour incubation (Appl Microbio
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| - | Biotechnol 2009 81:571- 578).
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| - | The process that was developed by Agnès Rodrigues’ team ensures the decontamination
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| - | of cobalt up to 0,5 ppm (8 nM in 100 000L) with only 4kg of bacteria as against 50kg with
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| - | an unmodified bacterium or 8,000kg of an ion-exchange polymer. This kind of process with
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| - | modified bacteria will be a good value because the production of bacteria in a bioreactor is
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| - | rather economical. However, one issue remained unsolved at the end of this study, that is the
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| - | separation of cobalt-fixing bacteria.
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| - | The first objective of our project is, with the most recent genetic engineering techniques, to
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| - | induce the fixation of optimized bacteria for the cobalt capture and retention in response to the
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| - | presence of contaminants in the effluent to be treated.
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| - | A second objective aims to develop a system to construct custom-built “biofilm inducible”
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| - | strains. Our goal is to construct captors able to launch the formation of biofilm in response to
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| - | the presence of various radioactive or not pollutants, and to offer more efficient and cheaper
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| - | bioremediation processes.
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| - | To conclude, our objective is to deposit a part able to make any strains inducible to cobalt. In presence of this element, strains will become adherent and will form biofilm thanks to their curli.
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| - | </span>
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| - | </p>
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