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Copenhagen/25 July 2011
From 2011.igem.org
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* Digested psb1c3 | * Digested psb1c3 | ||
| - | * Transformed A2-1 | + | * Transformed A2-1 and A2-2 from the ligation thursday into psb1c3 |
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| + | * Transformed B1 into the expression vector | ||
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| + | * Made an overnight culture on the colonies we did the pcr on, because it again didn't look as we hoped, so we will do at plasmid prep to test if the ligation is there anyway | ||
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| + | [[Team:Copenhagen/Notebook|Back to Notebook]] | ||
Latest revision as of 14:49, 25 July 2011
Monday
This morning we have found out that the pcr machine we used thursday is broken, so that can be the explanation for why the results friday were bad.
Labwork
- Do the pcr again and test on a gel
- Digested psb1c3
- Transformed A2-1 and A2-2 from the ligation thursday into psb1c3
- Transformed B1 into the expression vector
- Made an overnight culture on the colonies we did the pcr on, because it again didn't look as we hoped, so we will do at plasmid prep to test if the ligation is there anyway
