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Team:Grinnell/Notebook/Gels/Promoters
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| - | + | Figure 1: Promoters P<sub>xyl</sub> (inducible) and P<sub>rsaA</sub> (constitutive). Lane 1: ladder; lane 2: P<sub>rsaA</sub>; Lane 3: P<sub>xyl</sub>. Bands appear, but are hazy and spread out due to the small size of DNA fragments. | |
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| - | PCR products of <i>Caulobacter</i> promoters P<sub>rsaA</sub> and Pxyl. Lane 1: ladder; lane 2: P<sub>rsaA</sub>; lane 3: P<sub>xyl</sub>. | + | Figure 2: PCR products of <i>Caulobacter</i> promoters P<sub>rsaA</sub> and Pxyl. Lane 1: ladder; lane 2: P<sub>rsaA</sub>; lane 3: P<sub>xyl</sub>. |
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| - | Gel confirming that clean up did not remove DNA fragments. Lane 1: ladder; lane 2: P<sub>rsaA</sub>; lane 3: P<sub>xyl</sub>. | + | Figure 3: Gel confirming that clean up did not remove DNA fragments. Lane 1: ladder; lane 2: P<sub>rsaA</sub>; lane 3: P<sub>xyl</sub>. |
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==2011.06.26-2011.07.02== | ==2011.06.26-2011.07.02== | ||
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| + | Test to ensure all of our restriction enzymes are still active. Lane 1: ladder; lane 2: cut with Eco RI; lane 3: cut with XbaI; lane 4: standard uncut DNA fragment for lanes 2 and 3; lane 5: cut with SpeI; lane 6: cut with PstI; lane 7: standard uncut DNA fragment for lanes 5 and 6. There is an apparent decrease in size from standard DNA fragments to the digested samples. In the digest lanes there is also a dim band fairly far down the gel corresponding to the small end piece that is the other product of digestion. | ||
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Latest revision as of 17:03, 5 August 2011
Gels for Promoters
2011.06.12-2011.06.18
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| Figure 1: Promoters Pxyl (inducible) and PrsaA (constitutive). Lane 1: ladder; lane 2: PrsaA; Lane 3: Pxyl. Bands appear, but are hazy and spread out due to the small size of DNA fragments. | Figure 2: PCR products of Caulobacter promoters PrsaA and Pxyl. Lane 1: ladder; lane 2: PrsaA; lane 3: Pxyl. | Figure 3: Gel confirming that clean up did not remove DNA fragments. Lane 1: ladder; lane 2: PrsaA; lane 3: Pxyl. |
2011.06.19-2011.06.25
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| Gel of Plasmid PCR of promoters. Presence of two small bands shows contamination of samples. Lane 1: ladder; lanes 2-5: plasmid PCR of transformation survivors. | Gel of BBa_K081005 digests from minipreps. Lane 1: ladder; lane 2: digest of miniprep from overnights that should carry the desired promoter insert. Digest shows one band: the linearized plasmid. | Lane 1: ladder; lanes 2-4: plasmid PCR product of various promoter insertions into pSB1C3. Smearing due to leftover circular plasmid. Insertions seem to have been successful. |
2011.06.26-2011.07.02
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| Test to ensure all of our restriction enzymes are still active. Lane 1: ladder; lane 2: cut with Eco RI; lane 3: cut with XbaI; lane 4: standard uncut DNA fragment for lanes 2 and 3; lane 5: cut with SpeI; lane 6: cut with PstI; lane 7: standard uncut DNA fragment for lanes 5 and 6. There is an apparent decrease in size from standard DNA fragments to the digested samples. In the digest lanes there is also a dim band fairly far down the gel corresponding to the small end piece that is the other product of digestion. |
