Team:Berkeley

From 2011.igem.org

(Difference between revisions)
 
(436 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<html xmlns="http://www.w3.org/1999/xhtml"
 +
      xmlns:og="http://ogp.me/ns#"
 +
      xmlns:fb="https://www.facebook.com/2008/fbml">
 +
  <head>
 +
    <title>Berkeley iGEM 2011 </title>
 +
    <meta property="og:title" content="Berkeley iGEM 2011"/>
 +
    <meta property="og:type" content="university"/>
 +
    <meta property="og:description"
 +
          content="Berkeley's 2011 iGEM team wiki."/>
 +
<meta name="description" content="Berkeley iGEM 2011" />
 +
<meta name="keywords" content="igem, berkleley, 2011" />
-
<html>
+
<link rel="stylesheet" href="https://2011.igem.org/Template:Team:Berkeley/nivocss?action=raw&ctype=text/css" type="text/css"/>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.3.2/jquery.min.js"></script>
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #b6b6b6; padding: 5px;">
+
 
-
This is a template page. READ THESE INSTRUCTIONS.
+
<script type="text/javascript" src="https://2011.igem.org/Template:Team:Berkeley/javascript/javanivoslider?action=raw&ctype=text/javascript"></script>
 +
  </head>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2011/4/48/NavigationBar_Berkeley.jpg" alt="header" border="0"  usemap="#igemmap" width="1000"></center></div>
 +
 
 +
<map name="igemmap">
 +
  <area  shape="circle" coords="480,40,40" alt="Mercury" href="https://2011.igem.org" onfocus="this.blur()" />
 +
</map>
 +
 
 +
 
 +
<div class="layout-1000">
 +
<div class="row">
 +
<div class="col12" >
 +
<ul class="topnav">
 +
    <li><a href="https://2011.igem.org/Team:Berkeley">Home  </a></li>
 +
    <li>
 +
        <a href="https://2011.igem.org/Team:Berkeley/Project">Project</a>
 +
    </li>
 +
    <li>
 +
        <a href="https://2011.igem.org/Team:Berkeley/Parts">Parts</a>
 +
    </li>
 +
    <li><a href="https://2011.igem.org/Team:Berkeley/Safety">Safety</a></li>
 +
    <li><a href="https://2011.igem.org/Team:Berkeley/Team">Team</a></li>
 +
    <li><a href="https://2011.igem.org/Team:Berkeley/Judging">Judging</a></li>
 +
    <li><a href="https://2011.igem.org/Team:Berkeley/Notebooks">Notebooks</a></li>
 +
</ul>
 +
<div> <div class ="row-end"> </div>
</div>
</div>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
</div>
</div>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
 
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
+
<div class="row">
 +
<div class="col12" id="spacer2"></div>
 +
<div class="row-end"> </div>
</div>
</div>
 +
 +
 +
<div class="row">
 +
<div class="col6" align = "justify" >
 +
<img src="https://static.igem.org/mediawiki/2011/1/12/ProjectSummaryHeader.jpg" width="480">
 +
<p>Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological sensing devices. ToxS causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved toxic to E. coli. We built a stress-regulated transcription system that drives relatively high expression of toxic proteins. This allowed us to further engineer ToxR chimeras. We fused an estrogen-dependent dimer with ToxR hoping to create an estrogen biosensor. We observed a range of constitutive phenotypes and plan more experiments to engineer a dose-dependent transcriptional response to estrogen. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be used to build new biosensors. </p>
 +
</div>
 +
<div class="col6">
 +
<object width="480" height="300"><param name="movie" value="http://www.youtube.com/v/wuAlhXGKABA?"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><embed src="http://www.youtube.com/v/wuAlhXGKABA?" type="application/x-shockwave-flash" width="480" height="300" allowscriptaccess="always" allowfullscreen="true"></embed></object> </div>
 +
<div class ="row-end"> </div>
</div>
</div>
-
</html>
 
-
HELLO WORLD
 
-
<!-- *** End of the alert box *** -->
 
-
@charset "utf-8";
+
<div class="row">
-
body {
+
<div class="col12" id="spacer"></div>
-
margin:0;
+
<div class="row-end"> </div>
-
padding:0;
+
</div>
-
width:100%;
+
-
color:#959595;
+
-
font:normal 12px/1.8em Arial, Helvetica, sans-serif;
+
-
}
+
-
html, .main {
+
-
padding:0;
+
-
margin:0;
+
-
background:#fbfbfb;
+
-
}
+
-
.clr {
+
-
clear:both;
+
-
padding:0;
+
-
margin:0;
+
-
width:100%;
+
-
font-size:0;
+
-
line-height:0;
+
-
}
+
-
.logo {
+
-
height:98px;
+
-
float:left;
+
-
}
+
-
h1 {
+
-
margin:0;
+
-
padding:17px 0;
+
-
color:#e1e9df;
+
-
font:bold 40px/1.2em Arial, Helvetica, sans-serif;
+
-
letter-spacing:-2px;
+
-
}
+
-
h1 a, h1 a:hover {
+
-
color:#e1e9df;
+
-
text-decoration:none;
+
-
}
+
-
h1 span {
+
-
color:#d76002;
+
-
}
+
-
h1 small {
+
-
display:block;
+
-
padding:0;
+
-
font:normal 13px/1.2em Arial, Helvetica, sans-serif;
+
-
letter-spacing:normal;
+
-
}
+
-
h2 {
+
-
font:bold 24px Arial, Helvetica, sans-serif;
+
-
padding:8px 0;
+
-
margin:8px 0;
+
-
color:#323a3f;
+
-
}
+
-
p {
+
-
margin:8px 0;
+
-
padding:0 0 8px 0;
+
-
font:normal 12px/1.8em Arial, Helvetica, sans-serif;
+
-
}
+
-
p.spec {
+
-
text-align:right;
+
-
}
+
-
a {
+
-
color:#d76002;
+
-
text-decoration:underline;
+
-
}
+
-
a.rm, a.com {
+
-
padding:0;
+
-
text-decoration:none;
+
-
color:#fff;
+
-
}
+
-
.header, .content, .menu_nav, .fbg, .footer, form, ol, ol li, ul, .content .mainbar, .content .sidebar {
+
-
margin:0;
+
-
padding:0;
+
-
}
+
-
.fbg_resize {
+
-
margin:0 auto;
+
-
padding:0 40px;
+
-
width:890px;
+
-
}
+
-
.header {
+
-
background:#1c1c1c;
+
-
height:98px;
+
-
}
+
-
.header_resize {
+
-
margin:0 auto;
+
-
padding:0;
+
-
width:970px;
+
-
}
+
-
.hbg {
+
-
width:970px;
+
-
padding:20px 0;
+
-
}
+
-
.menu_nav {
+
-
margin:0;
+
-
padding:25px 0 0 20px;
+
-
float:right;
+
-
width:400px;
+
-
}
+
-
.menu_nav ul {
+
-
list-style:none;
+
-
}
+
-
.menu_nav ul li {
+
-
margin:0;
+
-
padding:0 2px 0 0;
+
-
float:left;
+
-
background:url(images/menu_line.gif) no-repeat right center;
+
-
}
+
-
.menu_nav ul li a {
+
-
display:block;
+
-
margin:0;
+
-
padding:12px 13px;
+
-
color:#7b7a7a;
+
-
text-decoration:none;
+
-
font-size:13px;
+
-
}
+
-
.menu_nav ul li.active a, .menu_nav ul li a:hover {
+
-
font-weight:bold;
+
-
color:#e58a42;
+
-
}
+
-
.content {
+
-
margin-bottom:12px;
+
-
}
+
-
.content_resize {
+
-
margin:0 auto;
+
-
padding:16px 0 0;
+
-
width:970px;
+
-
}
+
-
.mainbar {
+
-
margin:0;
+
-
padding:0;
+
-
float:right;
+
-
width:670px;
+
-
}
+
-
.mainbar img {
+
-
padding:4px;
+
-
border:1px solid #b7b7b7;
+
-
background-color:#fff;
+
-
}
+
-
.mainbar img.fl {
+
-
margin:4px 16px 4px 0;
+
-
float:left;
+
-
}
+
-
.mainbar .article {
+
-
margin:0;
+
-
padding:16px 24px 24px 40px;
+
-
}
+
-
.mainbar .post-data a {
+
-
color:#595959;
+
-
}
+
-
.mainbar .post-data a.com {
+
-
float:right;
+
-
color:#e58a42;
+
-
}
+
-
.mainbar .spec a {
+
-
background:#e58a42;
+
-
padding:10px;
+
-
}
+
-
.sidebar {
+
-
padding:0;
+
-
float:left;
+
-
width:300px;
+
-
background:#f7f7f7;
+
-
}
+
-
.sidebar .gadget {
+
-
margin:0;
+
-
padding:20px 16px 20px 30px;
+
-
}
+
-
ul.sb_menu, ul.ex_menu {
+
-
margin:0;
+
-
padding:0;
+
-
list-style:none;
+
-
color:#959595;
+
-
}
+
-
ul.sb_menu li, ul.ex_menu li {
+
-
margin:0;
+
-
}
+
-
ul.sb_menu li {
+
-
padding:4px 0 4px 12px;
+
-
width:220px;
+
-
}
+
-
ul.ex_menu li {
+
-
padding:4px 0 8px 12px;
+
-
}
+
-
ul.sb_menu li a, ul.ex_menu li a {
+
-
color:#5f5f5f;
+
-
text-decoration:none;
+
-
margin-left:-12px;
+
-
padding-left:12px;
+
-
background:url(images/menu_link.jpg) no-repeat left center;
+
-
}
+
-
ul.sb_menu li a:hover, ul.ex_menu li a:hover, ul.sb_menu li.active a, ul.ex_menu li.active a {
+
-
color:#d76002;
+
-
font-weight:bold;
+
-
background:url(images/menu_hover.jpg) no-repeat left center;
+
-
}
+
-
ul.sb_menu li a:hover {
+
-
text-decoration:underline;
+
-
}
+
-
ul.ex_menu li a:hover {
+
-
text-decoration:none;
+
-
}
+
-
.content .mainbar .comment {
+
-
margin:0;
+
-
padding:16px 0 0 0;
+
-
}
+
-
.content .mainbar .comment img.userpic {
+
-
border:1px solid #dedede;
+
-
margin:10px 16px 0 0;
+
-
padding:0;
+
-
float:left;
+
-
}
+
-
.fbg {
+
-
border-top:4px solid #e4e4e4;
+
-
background-color:#ededed;
+
-
}
+
-
.fbg_resize {
+
-
padding:24px 40px;
+
-
}
+
-
.fbg img {
+
-
padding:4px;
+
-
border:1px solid #cfd2d4;
+
-
background-color:#fff;
+
-
}
+
-
.fbg .col {
+
-
margin:0;
+
-
float:left;
+
-
}
+
-
.fbg .c1 {
+
-
padding:0 16px 0 0;
+
-
width:266px;
+
-
}
+
-
.fbg .c2 {
+
-
padding:0 16px;
+
-
width:300px;
+
-
}
+
-
.fbg .c3 {
+
-
padding:0 0 0 16px;
+
-
width:260px;
+
-
}
+
-
.fbg .c1 img {
+
-
margin:8px;
+
-
}
+
-
.fbg .c3 img {
+
-
margin:8px 16px 4px 0;
+
-
float:left;
+
-
}
+
-
.footer {
+
-
color:#959595;
+
-
background-color:#242424;
+
-
}
+
-
.footer_resize {
+
-
margin:0 auto;
+
-
padding:24px 40px;
+
-
width:890px;
+
-
}
+
-
.footer p {
+
-
margin:0;
+
-
padding:4px 0;
+
-
line-height:normal;
+
-
color:#959595;
+
-
}
+
-
.footer a {
+
-
color:#959595;
+
-
padding:inherit;
+
-
text-decoration:underline;
+
-
}
+
-
.footer a:hover {
+
-
text-decoration:none;
+
-
}
+
-
.footer .lf {
+
-
float:left;
+
-
}
+
-
.footer .rf {
+
-
float:right;
+
-
}
+
-
ol {
+
-
list-style:none;
+
-
}
+
-
ol li {
+
-
display:block;
+
-
clear:both;
+
-
}
+
-
ol li label {
+
-
display:block;
+
-
margin:0;
+
-
padding:16px 0 0 0;
+
-
}
+
-
ol li input.text {
+
-
width:480px;
+
-
border:1px solid #c0c0c0;
+
-
margin:2px 0;
+
-
padding:5px 2px;
+
-
height:16px;
+
-
background:#fff;
+
-
}
+
-
ol li textarea {
+
-
width:480px;
+
-
border:1px solid #c0c0c0;
+
-
margin:2px 0;
+
-
padding:2px;
+
-
background:#fff;
+
-
}
+
-
ol li .send {
+
-
margin:16px 0 0 0;
+
-
}
+
-
#search span {
+
-
width:216px;
+
-
display:block;
+
-
background:#FFF;
+
-
height:29px;
+
-
margin:8px 0 0;
+
-
padding:0;
+
-
border:1px solid #e9eaea;
+
-
}
+
-
#search input#s {
+
-
float:left;
+
-
width:170px;
+
-
padding:7px 0 7px 10px;
+
-
margin:0;
+
-
border:0;
+
-
background:none;
+
-
color:#909090;
+
-
font:normal 12px/15px Arial, Helvetica, sans-serif;
+
-
}
+
-
#search .btn {
+
-
float:left;
+
-
padding:0;
+
-
margin:0;
+
-
border:0;
+
-
width:auto;
+
-
}
+
 +
<div class="row">
-
{|align="justify"
+
<div class="col3-2"style="background-color:#101a4d;"><a href="https://2011.igem.org/Team:Berkeley/Project#ToxR"> <img src="https://static.igem.org/mediawiki/igem.org/1/10/Subtitlepic1.jpg"
-
|You can write a background of your team hereGive us a background of your team, the members, etc. Or tell us more about something of your choosing.
+
onmouseover="this.src='https://static.igem.org/mediawiki/igem.org/e/ef/Subtitlepic1hover.jpg'"
-
|[[Image:Berkeley_logo.png|200px|right|frame]]
+
onmouseout="this.src='https://static.igem.org/mediawiki/igem.org/1/10/Subtitlepic1.jpg'" width="230"></a>
-
|-
+
  <p style="text-align:center; color:#CECECE;"> A protein with great potential as a general biosensor system.</p> </div>
-
|
+
 
-
''Tell us more about your project. Give us backgroundUse this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
+
<div class="col3-2"style="background-color:#185f73;"><a href="https://2011.igem.org/Team:Berkeley/Project#ToxRChimera"><img src="https://static.igem.org/mediawiki/2011/b/bb/Subtitlepic3header.jpg"
-
|[[Image:Berkeley_team.png|right|frame|Your team picture]]
+
onmouseover="this.src='https://static.igem.org/mediawiki/2011/2/26/Subtitlepic3headerhover.jpg'"
-
|-
+
onmouseout="this.src='https://static.igem.org/mediawiki/2011/b/bb/Subtitlepic3header.jpg'" width="230"> </a>
-
|
+
<p style="text-align:center; color:#CECECE;"> Chimeric proteins that drive translation off of the Pctx promoter.</p></div>
-
|align="center"|[[Team:Berkeley | Team Example]]
+
 
-
|}
+
<div class="col3-2" style="background-color:#303285"><a href="https://2011.igem.org/Team:Berkeley/Project#StressPromoters"><img src="https://static.igem.org/mediawiki/2011/2/2b/Subtitlepic2header.jpg"
 +
onmouseover="this.src='https://static.igem.org/mediawiki/2011/3/32/Subtitlepic2headerhover.jpg'"
 +
onmouseout="this.src='https://static.igem.org/mediawiki/2011/2/2b/Subtitlepic2header.jpg'" width="230"></a>
 +
<p style="text-align:center; color:#CECECE;"> Our method for expressing interesting (but toxic) proteins.</p> </div>
 +
 
 +
<div class="col3-2"style="background-color:#499394"><a href="https://2011.igem.org/Team:Berkeley/Project#EstrogenBiosensor"><img src="https://static.igem.org/mediawiki/2011/c/c8/Subtitlepic4.jpg"
 +
onmouseover="this.src='https://static.igem.org/mediawiki/2011/1/12/Subtitlepic4hover.jpg'"
 +
onmouseout="this.src='https://static.igem.org/mediawiki/2011/c/c8/Subtitlepic4.jpg'" width="230"></a>
 +
  <p style="text-align:center; color:#CECECE;"> Bacteria engineered to detect the presence of estrogen. <br></p></div>
 +
<div class ="row-end"> </div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col12" id="spacer"></div>
 +
<div class="row-end"> </div>
 +
</div>
 +
 
 +
<br>
 +
<div class="row">
 +
 
 +
<div class="col4" align = "justify">
 +
<img src="https://static.igem.org/mediawiki/2011/b/bd/Aboutus.jpg">
 +
<p>We are Team Berkeley, a cohesive unit of 7 undergraduates and 3 advisers. Earlier this year we planned a complex and risky project, given the short amount of time iGEM made available. We quickly learned each others strengths and weaknesses and developed standard systems of organizational management in order to synchronize our efforts for the many parallel tasks at hand. We created protocols, shared them with one another, and worked together on troubleshooting. Using google docs to keep up with the status of cloning projects, the results of assays, material logistics, or the final steps required to complete a project ensured that through the months of hard work, we fine-tuned our ability to work together. As a team, we have learned firsthand how the synthetic biology community relies on the goal-oriented cooperation of skilled individuals from very different backgrounds and with very different skill sets. Some of us have strong engineering backgrounds while others of us have strong biology backgrounds, but we all share a desire to build synthetic biological systems that solve human problems. We are proud of the project that we have created which we will present at the Jamboree together in October.</p>
 +
</div>
 +
 
 +
<div class = "col14">
 +
<p>
 +
</p>
 +
</div>
 +
 
 +
<div class="col8-4">
 +
 
 +
        <div class="slider-wrapper theme-default">
 +
            <div class="ribbon"></div>
 +
            <div id="slider" class="nivoSlider">
 +
                <img src="https://static.igem.org/mediawiki/2011/c/ce/Slideshow1.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/e/ea/Slideshow2.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/d/db/Slideshow3.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/d/db/Slideshow4.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/d/d0/Slideshow5.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/2/2c/Slideshow6.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/f/f5/Slideshow7.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/b/b5/Slideshow14.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/7/71/Slideshow15.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/1/1a/Slideshow14.JPG" />
 +
                <img src="https://static.igem.org/mediawiki/2011/b/b2/Slideshow10.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/2/28/Slideshow12.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/9/9b/Slideshow13.jpg" />
 +
                <img src="https://static.igem.org/mediawiki/2011/9/94/Slideshow11.jpg" />
 +
            </div>
 +
    </div>
 +
 
 +
    <script type="text/javascript">
 +
  $(window).load(function() {
 +
        $('#slider').nivoSlider();
 +
    });
 +
    </script>
 +
<br>
 +
 
 +
<div id="fb-root"></div>
 +
<script>(function(d, s, id) {
 +
  var js, fjs = d.getElementsByTagName(s)[0];
 +
  if (d.getElementById(id)) {return;}
 +
  js = d.createElement(s); js.id = id;
 +
  js.src = "//connect.facebook.net/en_US/all.js#xfbml=1";
 +
  fjs.parentNode.insertBefore(js, fjs);
 +
}(document, 'script', 'facebook-jssdk'));</script>
 +
 
 +
<div class="fb-like" data-href="https://2011.igem.org/Team:Berkeley" data-send="true" data-width="600" data-show-faces="false" data-font="lucida grande"></div>
 +
 
 +
</div>
 +
<div class="row-end"></div>
 +
</div>
 +
 
 +
<div class="row">
 +
<div class="col12" id="spacer"></div>
 +
<div class="row-end"> </div>
 +
</div>
 +
 
 +
 
 +
<div class = "row">
 +
<div class="col12-3" style="background-color:#499394">
 +
<p style="text-align:left; color:#CECECE; font-size:13px; font-weight:bold; padding:3px"> The UC Berkeley iGEM team would like to thank Autodesk and Agilent for their financial support and Synberc, for their administrative support. </p>
 +
 
 +
</div>
 +
<div class="row-end"> </div>
 +
</div>
 +
 
 +
 
 +
<div class="row">
 +
<div class="col12" id="spacer"></div>
 +
<div class="row-end"> </div>
 +
</div>
 +
 
 +
<div class = "row">
 +
<div class="col12-3" >
 +
<img src="https://static.igem.org/mediawiki/2011/f/f1/Agilentlogo.png" width="300" >
 +
<img src="https://static.igem.org/mediawiki/2011/b/bc/SynBERC_logo_poster.png" width="300" >
 +
<img src="https://static.igem.org/mediawiki/2011/e/e8/Autodesk_logo_blk_l.jpg"width="300">
 +
<div class="row-end"></div>
 +
</div>
 +
 
 +
</div>
 +
</html>
-
<!--- The Mission, Experiments --->
 
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
{{Team:Berkeley/header}}
-
!align="center"|[[Team:Berkeley|Home]]
+
{{Team:Berkeley/css}}
-
!align="center"|[[Team:Berkeley/Team|Team]]
+
{{Team:Berkeley/navigation}}
-
!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Berkeley Official Team Profile]
+
-
!align="center"|[[Team:Berkeley/Project|Project]]
+
-
!align="center"|[[Team:Berkeley/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:Berkeley/Modeling|Modeling]]
+
-
!align="center"|[[Team:Berkeley/Notebook|Notebook]]
+
-
!align="center"|[[Team:Berkeley/Safety|Safety]]
+
-
!align="center"|[[Team:Berkeley/Attributions|Attributions]]
+
-
|}
+

Latest revision as of 03:39, 29 September 2011

Berkeley iGEM 2011

header
Mercury

Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological sensing devices. ToxS causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved toxic to E. coli. We built a stress-regulated transcription system that drives relatively high expression of toxic proteins. This allowed us to further engineer ToxR chimeras. We fused an estrogen-dependent dimer with ToxR hoping to create an estrogen biosensor. We observed a range of constitutive phenotypes and plan more experiments to engineer a dose-dependent transcriptional response to estrogen. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be used to build new biosensors.

A protein with great potential as a general biosensor system.

Chimeric proteins that drive translation off of the Pctx promoter.

Our method for expressing interesting (but toxic) proteins.

Bacteria engineered to detect the presence of estrogen.


We are Team Berkeley, a cohesive unit of 7 undergraduates and 3 advisers. Earlier this year we planned a complex and risky project, given the short amount of time iGEM made available. We quickly learned each others strengths and weaknesses and developed standard systems of organizational management in order to synchronize our efforts for the many parallel tasks at hand. We created protocols, shared them with one another, and worked together on troubleshooting. Using google docs to keep up with the status of cloning projects, the results of assays, material logistics, or the final steps required to complete a project ensured that through the months of hard work, we fine-tuned our ability to work together. As a team, we have learned firsthand how the synthetic biology community relies on the goal-oriented cooperation of skilled individuals from very different backgrounds and with very different skill sets. Some of us have strong engineering backgrounds while others of us have strong biology backgrounds, but we all share a desire to build synthetic biological systems that solve human problems. We are proud of the project that we have created which we will present at the Jamboree together in October.


The UC Berkeley iGEM team would like to thank Autodesk and Agilent for their financial support and Synberc, for their administrative support.