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Uppsala-Sweden/12 July 2011
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| + | 2011-07-12 | ||
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| + | The day started by running a gel of ccaS mutant, amilCP-BB10, and amilGFP-PstI. At the same time we prepared the sample (ccaR, ccaS, RBS-ho1 and RBS-pcyA) for sequencing. Plating of terminator B1001, PLlacO, pSB3T5 and pSB4K5. PCR purification of ccaS, amilGFP, amilCP, clone amilCP and PcpcG2 (Purification protocol). Re-ligation and transform mutagenized ccaS and amilGFP. Transformation of amilCP, clone amilCP, TetR inverter and pcpcG2. Re-streak of cI inverter transformants from 2011-07-11. | ||
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| + | The transformation of inverters from yesterday didn’t go well. The lambda-cI-inverter had only a few colonies, while the tetR inverter had no colonies at all. So TetR inverter has to be redone. This time the transformation used 2 µl of DNA from the MIT distribution. | ||
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| + | Furthermore new plates containing all antibiotics and some containing teracyclin were made. | ||
Latest revision as of 20:26, 20 July 2011
Welcome to Uppsala-SwedeniEM '2011
Notebook
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2011-07-12
The day started by running a gel of ccaS mutant, amilCP-BB10, and amilGFP-PstI. At the same time we prepared the sample (ccaR, ccaS, RBS-ho1 and RBS-pcyA) for sequencing. Plating of terminator B1001, PLlacO, pSB3T5 and pSB4K5. PCR purification of ccaS, amilGFP, amilCP, clone amilCP and PcpcG2 (Purification protocol). Re-ligation and transform mutagenized ccaS and amilGFP. Transformation of amilCP, clone amilCP, TetR inverter and pcpcG2. Re-streak of cI inverter transformants from 2011-07-11.
The transformation of inverters from yesterday didn’t go well. The lambda-cI-inverter had only a few colonies, while the tetR inverter had no colonies at all. So TetR inverter has to be redone. This time the transformation used 2 µl of DNA from the MIT distribution.
Furthermore new plates containing all antibiotics and some containing teracyclin were made.
