From 2011.igem.org
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- | =Isolating Reflectin DNA=
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- | Obtaining DNA coding for reflectin proteins was the vital first step in our project. [http://en.wikipedia.org/wiki/Loligo Loligo] tissue was sourced from fishing bait suppliers and culinary wholesalers in order to attempt genomic DNA extraction. From our literature search we discovered that the reflectin gene from E. scolopes contains no introns, so genomic DNA should be suitable for expression in E. coli.
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- | ==Squid Dissection and Tissue Samples==
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- | Specimens of what we identified as [http://www.marlin.ac.uk/speciesinformation.php?speciesID=3718 ''Loligo vulgaris''] and [http://en.wikipedia.org/wiki/Opalescent_Inshore_Squid ''Loligo opalescens''] were dissected for examination.
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Latest revision as of 14:22, 20 July 2011