File:20110713 BD1+pMR10 DRDX.jpg

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(<html> Gel of colony PCR of transformants that should carry BBa_K081005, WT esp, and rsaA in pMR10, P<sub>xyl</sub>, WT esp, and rsaA in pSB1C3, or P<sub>rsaA</sub>, WT esp, and rsaA in pSB1C3. Lane 1: ladder; lanes 2-5: BBa_K081005, WT esp, and rsaA in p)
 
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Gel of colony PCR of transformants that should carry BBa_K081005, WT esp, and rsaA in pMR10, P<sub>xyl</sub>, WT esp, and rsaA in pSB1C3, or P<sub>rsaA</sub>, WT esp, and rsaA in pSB1C3. Lane 1: ladder; lanes 2-5: BBa_K081005, WT esp, and rsaA in pMR10; lanes 6,7: P<sub>xyl</sub>, WT esp, and rsaA in pSB1C3; lanes 8,9: P<sub>rsaA</sub>, WT esp, and rsaA in pSB1C3.  Transformant in lane 4 was successful.
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Gel of colony PCR of transformants that should carry BBa_K081005, WT <i>esp</i>, and <i>rsaA</i> in pMR10, P<sub>xyl</sub>, WT <i>esp</i>, and <i>rsaA</i> in pSB1C3, or P<sub>rsaA</sub>, WT <i>esp</i>, and <i>rsaA</i> in pSB1C3. Lane 1: ladder; lanes 2-5: BBa_K081005, WT <i>esp</i>, and <i>rsaA</i> in pMR10; lanes 6,7: P<sub>xyl</sub>, WT <i>esp</i>, and <i>rsaA</i> in pSB1C3; lanes 8,9: P<sub>rsaA</sub>, WT <i>esp</i>, and <i>rsaA</i> in pSB1C3.
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Latest revision as of 19:46, 22 July 2011

Gel of colony PCR of transformants that should carry BBa_K081005, WT esp, and rsaA in pMR10, Pxyl, WT esp, and rsaA in pSB1C3, or PrsaA, WT esp, and rsaA in pSB1C3. Lane 1: ladder; lanes 2-5: BBa_K081005, WT esp, and rsaA in pMR10; lanes 6,7: Pxyl, WT esp, and rsaA in pSB1C3; lanes 8,9: PrsaA, WT esp, and rsaA in pSB1C3.

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current20:20, 19 July 2011Thumbnail for version as of 20:20, 19 July 2011938×836 (15 KB)Aaring (Talk | contribs) (<html> Gel of colony PCR of transformants that should carry BBa_K081005, WT esp, and rsaA in pMR10, P<sub>xyl</sub>, WT esp, and rsaA in pSB1C3, or P<sub>rsaA</sub>, WT esp, and rsaA in pSB1C3. Lane 1: ladder; lanes 2-5: BBa_K081005, WT esp, and rsaA in p)

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