Team:UT Dallas

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      <h1><a href="index.html"><span>UTDallas</span> iGem 2011<small></small></a></h1>
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        <h3>Creating Futures</h3>
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          <h2><span><b>UT Dallas iGem 2011-</b> Welcome to Our Wiki!</span></h2>
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          <!--<p class="post-data"><a href="#" class="com fr"></a> <span class="date">March 16, 2018</span> &nbsp;|&nbsp; Posted by <a href="#">Owner</a> &nbsp;|&nbsp; Filed under <a href="#">templates</a>, <a href="#">internet</a></p>-->
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<h2>Immunobots: a step towards intelligent probiotics</h2>
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<br>
 +
<table width="720">
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<tr><td valign="top" >
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The human bowel hosts a rich diversity of symbiotic microflora that provides a powerful
 +
engineering platform for intelligent probiotics. These immunobots will ideally work in-sync
 +
and enhance natural self-repair mechanisms for a range of intestinal diseases associated with
 +
tissue damage.<br><br>Towards this end, we engineered a bacterial chemotaxis pathway that utilizes a
 +
chimeric receptor to successfully interface with the immune system (<b>immunobot</b>). In addition, we introduced
 +
an inducible secondary bacterial population that can trigger system-wide self-destruction,
 +
conferring an additional level of user control (<b>killbot</b>). Each module of our system was characterized
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using fluorescent reporters and the integrated parts were evaluated by controlled experiments
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involving wound signal gradients.<br><br>We envision a probiotic solution that can facilitate localized
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tissue repair for damage resulting from inflammatory bowel diseases, including ulcerative colitis
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and Crohn’s disease.</td>
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<img src="https://static.igem.org/mediawiki/2011/2/2b/IGEM2011.jpg" width="200" style="margin-left: 26px; border: 1px solid #8E8E8E">
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<div class="rounded"></div>
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          <p>This is where we will put the project decription. Above this paragraph, in the white box/picture that won't show up, we will put a team picture. Below this paragraph, there is a blue "Read More" link that will link to the project page. Maybe I can get the picture to link to the team page. In the section right below this we can put the safety proposal that's due July 15th along with the project description, and we can put it right below this just to make sure they see it- and then we can move it later.</p>
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          <p class="spec"> <a href="https://2011.igem.org/Team:UT_Dallas/Project2" class="rm fl"><font size="4" face="verdana">Read more</a></font></p>
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          <h2><span></span> Safety Proposal</h2>
 
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        <!-- <p class="post-data"><a href="#" class="com fr"></a> <span class="date">March 15, 2010</span> &nbsp;|&nbsp; Posted by <a href="#">Owner</a> &nbsp;|&nbsp; Filed under <a href="#">templates</a>, <a href="#">internet</a></p>-->
 
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        <!-- <img src="images/images_2.jpg" width="613" height="193" alt="" />-->
 
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          <p><b>1. Would any of your project ideas raise safety issues in terms of researcher, public, or environmental safety?</b></p>
 
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<blockquote>Materials that pose a safety risk (i.e. cell cultures, ethidium bromide, UV transilluminator, etc) are handled according to standard lab safety protocol and Materials Safety Data Sheets. Materials containing safety hazards are disposed in separate containers with “biohazard” designations. Appropriate protective gear, such as gloves, is worn at all times. Our bacterial chassis, E. coli DH5α, is disabled to where it is nonpathogenic and cannot survive outside of lab conditions. In addition, all exposed counters are disinfected using 70% ethanol after each use of the lab. All lab equipment is thoroughly cleaned and autoclaved after use and access to the lab is limited by cardkey.</blockquote>
 
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<p><b>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?</b></p>
 
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<blockquote>We worked with genes encoding known properties and utilized safety measures to ensure that biohazardous materials including antibiotic-resistant cells are contained within the lab and are appropriately disposed.  Therefore, we assess that none of our submitted parts raise safety issues.</blockquote>
 
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<p><b>3. Is there a local biosafety group, committee, or review board at your institution?</b></p>
 
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<blockquote>UT Dallas has an Institutional Biosafety Committee that manages all safety responsibilities under NIH “Guidelines for Research Involving Recombinant DNA Molecules”. Throughout the course of this work, we ensured that all lab activity respected safety measures. </blockquote>
 
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<p><b>4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?</b></p>
 
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<blockquote>The iGEM plasmid backbone could be programmed to include an "inactivation mechanism" whose induction can be used to regulate the activity of cells endowed with BioBrick parts. Disseminating pertinent safety information through the Registry is a practical alternative to engineering biosafety measures into parts, devices and systems.</blockquote>
 
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<p>...and then we can put our real introduction to our project right here</p>
 
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          <p class="spec"><a href="#" class="rm fl"><font size="4" face="verdana">Read more</a></font></p>
 
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            <li class="active"><a href="https://2011.igem.org/Team:UT_Dallas"><font size="3" face="verdana">Home</a></font></li>
 
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            <p><span class="q"><img src="https://static.igem.org/mediawiki/2011/8/85/Quote_1.gif" width="16" height="14" alt="" /></span> Twitter embed <span class="q"><img src="https://static.igem.org/mediawiki/2011/a/a1/Quote_2.gif" width="16" height="14" alt="" /></span></p>
 
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      <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/eb/Pic_1.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/8/87/Pic_2.jpg
 
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" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/e/e8/Pic_3.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/2a/Pic_4.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/1/17/Pic_5.jpg" width="58" height="58" alt="" /></a> <a href="#"><img src="https://static.igem.org/mediawiki/2011/7/76/Pic_6.jpg" width="58" height="58" alt="" /></a> </div>
 
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      <h2><span><font size="5" face="verdana">UT Dallas</span></font></h2>
 
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      <p>Want to learn more about UTD?<br />
 
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      A little portal to learn more about UTD if anyone wants to. Learn more about where we work and some fun facts about UTD. *Add picture of Nserl.</p><p><a href="#"><font size="3" face="verdana">Click here to learn more about UTD</a></font></p>
 
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      <h2><span><font size="5" face="verdana">Notebook</span></font></h2>
 
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      <p>This will just give a brief description of what we've been doing and then link to the Notebook page when anyone clicks the "Learn More" link. See what we've been doing over the past few months.
 
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</p><p> <a href="#"><font size="3" face="verdana">Learn more...</a></font></p>
 
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Latest revision as of 03:29, 29 September 2011

Retrieved from "http://2011.igem.org/Team:UT_Dallas"