Team:Peking S

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A ‘Chemical Wire’ Toolbox for Synthetic Microbial Consortia

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Project presentation
Cell-cell communication-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient. Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
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-  <p align="center"><font face="Castellar" class="ws20"><B>PROJECT DESCRIPTION</B></font></p>
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-Cell-cell communication-based multicellular networks provide an extended vista for synthetic biology. By compartmentalizing complex genetic circuits into separate engineered cells, the difficulty of the construction by layering elementary gates can be dramatically reduced, partly due to the insulation of crosstalk between modules, the suppression of noise by populationally averaging, and the reducing of metabolic burden in host cells. What’s more, cell-cell communication-based multicellular feature enables coordination and synchronization among cells in and between populations and facilitates the generation of reliable non-Boolean dynamics.
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-However, orthogonal ‘chemical wires’ that allow concurrent communication are far from sufficient. Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics. Criteria and assays for ‘chemical wire’ characterization and validation have been established, in which the orthogonality, the speed of signal relaying and the ability to coordinate cells are verified. A set of recently reported quorum sensing systems have been selected, together with previously investigated AHL system, as ‘chemical wire’ toolbox. With this toolbox, multicellular design principles for complex logic circuit that allow a reduction in the complexity and a guarantee in both robust function and operation speed of a system will be proposed, followed by constructing reusable E.coli cell types that each compartmentalizes specific elementary logic gate in a single cell, connected by ‘chemical wires’. By layering a very few number of such cellular logic gates, complex logic computing process will be easily implemented. Besides, a queen-worker-interaction based cell density oscillator will be constructed, in order to verify the feasibility of our ‘chemical wire’ toolkit for non-Boolean dynamic functions. Nonetheless, challenges remain for this non-Boolean process because communication signals are produced by growing cells, i.e. changes in cell densities may affect the strength of communication. To crack these hurdles, we will develop a microfluidic device that imposes constraints on cell densities and on the flow of chemical signals to guarantee well-coordinated population dynamics.
+<div id="text1" style="position:absolute; overflow:hidden; left:130px; top:365px; width:700px;z-index:25;">
+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>A </span><span style="font-size:16px"><font color="#ffffff" class="ws16">&#8216;Chemical Wire&#8217; Toolbox for Synthetic Microbial Consortia</B></font></span></div>
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+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Click below</span><span style="font-size:16px"><font color="#000000" class="ws16"> To access a brief introduction of our project<br><br></B></font></span></div>
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+<b>Cell-cell communication</b>-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient.
+Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
+<br>
+<a href=https://2011.igem.org/Team:Peking_S/project>
+learn more
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+<div id="image7" style="position:absolute; overflow:hidden; left:455px; top:1182px; width:230px; height:37px; z-index:45"><a href="http://www.beijinglab.com.cn/"><img src="https://static.igem.org/mediawiki/2011/e/ec/PekingR_beijinglihua.jpg" alt="" title="" border=0 width=230 height=37></div>
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