Team:Uppsala-Sweden

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<span class="title">''Uppsala '' <span class="bold">University</span>.</span>
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<span class="hidden">Welcome to Uppsala-SwedeniEM '2011</span>
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Abstract
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Show color with color. The aim is to control gene expression by using only light. Our light sensing system is unique. The output modules will, when right sensor is active, express a colorfull picture.[[Team:Uppsala-Sweden/Project |(More)]]
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=== About our project ===
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Team
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The team consists of 18 enthusiastic undergraduate students from the Masters Programme in Molecular Biotechnology Engineering and Bioinformatics. Together we will represent ....[[Team:Uppsala-Sweden/Team|(More)]]
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Assembly plan
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The assembly plan shows the approach of our project. It is divided in seven parts (red output, blue output, green/yellow output, chromophore, red sensor, blue sensor and green sensor). [[Team:Uppsala-Sweden/Assembly_plan|(More)]]
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                <img src="https://static.igem.org/mediawiki/2011/b/b1/B29_UU.jpg" title="''Pure PCR-products to be sequenced''"/>
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                <img src="https://static.igem.org/mediawiki/2011/0/03/B36_UU.JPG" title="" />
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                <img src="https://static.igem.org/mediawiki/2011/2/28/MG_6276.jpeg" title="Photo by Mikael Wallerstedt for the magazine Universen"/>
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                <img src="https://static.igem.org/mediawiki/2011/b/b3/MG_6291.jpeg" title="Photo by Mikael Wallerstedt for the magazine Universen"/>
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About our project
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<!--=== About our project ===
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The aim of our project is to regulate gene expression by three different wavelengths and further on build a bacterial system that will act like a bacterial photocopier. With this project we will participate in the International Genetically Engineered Machine competition (iGEM).<br><br>
The aim of our project is to regulate gene expression by three different wavelengths and further on build a bacterial system that will act like a bacterial photocopier. With this project we will participate in the International Genetically Engineered Machine competition (iGEM).<br><br>
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The laboratory part of this project is to further develop the light induced regulation of gene expression. We will start out by repeating the multichromatic experiments explained in the article Multichromatic Control of Gene Expression in Escherichia coli (Voigt et al. 2011) with minor modifications. The first step is to construct the DNA for at least three light-sensing systems, each with different activation wavelengths and different outputs. Then we test each light-sensing system individually in a bacteria platform. It means we test red, green and blue light sensing separately. Once this is done, we plan to express the three light-sensing systems in the same platform, at the same time and test their ability put together. The laboratory work includes tasks such as plasmid preparations, transformations, BioBrick cloning, mutagenesis and sequencing.<br><br>
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The experimental part of this project is to further develop the light induced regulation of gene expression, aka coliroids. We start out by employing the principles explained by Tabor, Levskaya and Voigt's research on multichromatic light sensing. The first step is make DNA constructs for at least three light-sensing systems, each with different activation wavelengths and different outputs. Then we test each light-sensing system individually in a bacteria platform. Once this is done, we plan to express the three light-sensing systems in the same platform, at the same time and test their abilities when put together. The laboratory methods include for instance BioBrick cloning, site-directed mutagenesis, Lambda Red recombineering, etc.<br><br>
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The ultimate goal is by these three different wavelengths make the bacterial cultures create a picture on an agar plate and thereby act like a photocopier.<br><br>
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The ultimate goal is making a "multichromatic coliroid system" capable of independent regulation of three sets of genes by light regulation. The proof of concept will be demonstrated by growing a colorful picture on an agar plate. <br><br>
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Besides this, we will also put a lot of efforts into other parts of the project including software tool development, marketing, human practice biosecurity, bio-ethics and website.<br><br>
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Besides this, we will also put a lot of efforts into other parts of the project including characterization of parts, marketing, human practice biosecurity, bio-ethics and website.<br><br>
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For more information about previous Uppsala iGEM teams, have a look at [https://2009.igem.org/Team:Uppsala-Sweden UU-IGEM-2009 ] and
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You can read more about our system design at our data page:
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[https://2010.igem.org/Team:Uppsala-Sweden UU-IGEM-2010 ]
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https://2011.igem.org/Team:Uppsala-Sweden/Project/Data
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=== Latest updates ===
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== Blue Sensor - Animated Example ==
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{{Template:Uppsala-SwedenTemplatefooter}}

Latest revision as of 22:14, 28 October 2011

Visit our blog:

USblog.png










http://igem2011uu.blogspot.com



Abstract

Show color with color. The aim is to control gene expression by using only light. Our light sensing system is unique. The output modules will, when right sensor is active, express a colorfull picture.(More)

Team

The team consists of 18 enthusiastic undergraduate students from the Masters Programme in Molecular Biotechnology Engineering and Bioinformatics. Together we will represent ....(More)

Assembly plan

The assembly plan shows the approach of our project. It is divided in seven parts (red output, blue output, green/yellow output, chromophore, red sensor, blue sensor and green sensor). (More)




About our project

The aim of our project is to regulate gene expression by three different wavelengths and further on build a bacterial system that will act like a bacterial photocopier. With this project we will participate in the International Genetically Engineered Machine competition (iGEM).

The experimental part of this project is to further develop the light induced regulation of gene expression, aka coliroids. We start out by employing the principles explained by Tabor, Levskaya and Voigt's research on multichromatic light sensing. The first step is make DNA constructs for at least three light-sensing systems, each with different activation wavelengths and different outputs. Then we test each light-sensing system individually in a bacteria platform. Once this is done, we plan to express the three light-sensing systems in the same platform, at the same time and test their abilities when put together. The laboratory methods include for instance BioBrick cloning, site-directed mutagenesis, Lambda Red recombineering, etc.

The ultimate goal is making a "multichromatic coliroid system" capable of independent regulation of three sets of genes by light regulation. The proof of concept will be demonstrated by growing a colorful picture on an agar plate.

Besides this, we will also put a lot of efforts into other parts of the project including characterization of parts, marketing, human practice biosecurity, bio-ethics and website.

You can read more about our system design at our data page:

https://2011.igem.org/Team:Uppsala-Sweden/Project/Data


Blue Sensor - Animated Example






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