Team:DTU-Denmark-2

From 2011.igem.org

(Difference between revisions)
 
(190 intermediate revisions not shown)
Line 1: Line 1:
 +
{{:Team:DTU-Denmark-2/Header}}
<html>
<html>
-
<img src="https://static.igem.org/mediawiki/2011/4/45/Header_temp.jpg"  align="center"> </img>
+
 
<head>
<head>
-
<title>Welcome to the DTU iGEM wiki!</title>
+
<style type="text/css">
-
<style>
+
-
#globalWrapper {
+
-
background-image: "" !important;
+
-
background-repeat: repeat;
+
-
}
 
-
.ideasList{
+
 
-
margin-bottom:30px
+
div.flash
 +
{
 +
display: block;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
left:0px;
 +
top:0px;
 +
z-index:-200;
}
}
-
 
+
ul.dropdown * {
-
ul#sub {
+
        z-index: 200;
-
margin:0px;
+
-
padding:0px;
+
-
font-size:11px;
+
-
list-style-image:none;
+
-
list-style-type:none;
+
-
 
+
}
}
-
#sub li { margin-bottom:20px;}
+
  div.image {
 +
    padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    text-align:center;
 +
  }
 +
div.image2 {
 +
   
 +
    display: block;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
  }
-
#sub li a {
+
  div.slogan {
-
color:#000000FF;
+
  padding-right: 20px;
-
text-decoration:underline;
+
    padding-left: 20px;
-
}
+
    padding-bottom:20px;
 +
    padding-top:20px;
 +
    background-color:#FBFF74;
 +
    border-style: bold;
 +
    border-width: 1px;
 +
    border-color:#F2F2F2;
 +
    -webkit-box-shadow: 5px 5px 2px #888888; /* Safari */
 +
    box-shadow: 5px 5px 2px #888888;
 +
    font-style:italic;
 +
    font-weight:bold;
 +
    font-size:120%;
 +
    font-family:"arial";
-
#sub li a:hover {
 
-
text-decoration:none;
 
}
}
-
 
+
div.igemlogo img {
-
.links {
+
    margin: 20px 20px 20px 20px;
-
width:967px;
+
        }
-
background-color:white;
+
div.red {
-
margin-bottom:0px;
+
    padding-right: 30px;  
 +
    padding-left: 30px;
 +
    padding-bottom:30px;
 +
    padding-top:30px;
 +
    text-align:center;
 +
    background-color:#E7F9FF
}
}
 +
div.grey {
-
.links tr {
+
    padding-right: 5px;
-
background-color:#000000;
+
    padding-left: 5px;
-
height:30px;
+
    padding-bottom:5px;
 +
    padding-top:5px;
 +
    width: 260px;
 +
    height: 1150px;
 +
    background-color:#F9F9F9
 +
  }
 +
 +
div.green {
 +
    padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width: 189px;
 +
    height: 220px;
 +
    background-color:#D8F6CE;
 +
    float:left;
 +
    margin:5px;
 +
    line-height:120%;
}
}
-
 
+
div.blue {
-
.links td {
+
    padding-right: 10px;
-
padding: 0;
+
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width: 189px;
 +
    height: 220px;
 +
    line-height:120%;
 +
      background-color:#FFDEB6;
 +
float:left;
 +
margin:5px;
}
}
 +
div.yellow {
 +
    padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width: 189px;
 +
    height: 220px;
 +
    float:left;
 +
    margin:5px;
 +
line-height:120%;
 +
    background-color:#F6CED8;
 +
}
 +
div.purple {
 +
  padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width:  189px;
 +
    height:  220px;
 +
    float:left;
 +
    margin:5px;
 +
    line-height:120%;
 +
    background-color:#A9E2F3;
 +
}
 +
div.lightgreen {
 +
  padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width:  189px;
 +
    height:  220px;
 +
    line-height:120%;
 +
float:left;
 +
    margin:5px;
 +
   
 +
background-color:#FAFEBA;
 +
}
 +
div.pink {
 +
    padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width:  189px;
 +
    height:  220px;
 +
    line-height:120%;
 +
float:left;
 +
    margin:5px;
 +
    background-color:#CEF6E3;
 +
}
 +
<!--
 +
A{text-decoration:none}
 +
A:link {color:  #0000FF;text-decoration: none }
 +
A:active {color:  #0000FF;text-decoration: none }
 +
A:visited {color:  #0000FF ;text-decoration: none }
 +
A:hover {text-decoration: none}
 +
-->
 +
</style>
 +
</head>
-
.links a{
+
<body>
-
display: block;
+
-
width: 130px;
+
-
height: 100%;
+
-
margin: 0;
+
-
text-align: center;
+
-
text-decoration: none;
+
-
font-size: 15px;
+
-
color: #fff;
+
-
}
+
-
.links a:active {
 
-
}
 
-
.links a:hover {
+
<table> <!-- Table for content area -->
-
background-color: #999999;
+
-
}
+
 +
<!-- Main content area -->
 +
<table cellpadding=10px>
-
#header {
+
<td width="660px" height="100%" valign="top" >
-
width: 100%;
+
-
text-align: left;
+
-
}
+
-
h4 {
+
<div class="flash">
-
font-size: 13pt;
+
<object type="application/x-shockwave-flash" width="600" height="400" data="https://static.igem.org/mediawiki/2011/2/2b/Flash-anim-4_hurtig.swf">
-
font-weight: normal;
+
<param name="movie" value="https://static.igem.org/mediawiki/2011/2/2b/Flash-anim-4_hurtig.swf")>
-
}
+
<param name="wmode" value="transparent">
 +
</object>
-
dt {
+
</div>
-
font-size: 12pt;
+
-
}
+
-
#projectnav h4 {
+
<div class="red">
-
padding: .4ex;
+
-
}
+
-
#projectnav dt {
+
<p align="justify">
-
font-weight: normal;
+
<font color="#000000" face="arial" size="5" >
-
margin: 1.5ex 0 0 0;
+
<b>Making Molecular Biology Easier</b>
-
}
+
</font></p>
 +
<font color="#000000" face="arial" size="2" >
 +
<p align="justify">
 +
<br>
 +
<p align="justify">
 +
<b>We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and <i>Aspergilli </i> ready to plug 'n' play. You can find more information about how this standard works <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly">here</a>.<b>
 +
</p>
 +
</font>
 +
</div>
 +
<br>
-
#projectnav h4, #projectnav dt, #projectnav dd {
+
<div class="green">
-
text-align: left;
+
<font color="#000000" face="arial" size="3" >
-
text-indent: 0;
+
<b>A Reporter System</b><br><br>
-
margin-left: 0;
+
</font>
-
}
+
<div class="image2">
-
#hbnav dd {
 
-
padding: .8ex;
 
-
}
 
-
#denav dd{
+
<img src="https://static.igem.org/mediawiki/2011/8/84/IGEM_001.jpg" width="187px" height="50px"> </img><br>
-
padding: 1ex;
+
-
margin-top: 0;
+
-
}
+
-
#projectnav a {
+
</div>
-
color: #006699;
+
-
}
+
-
#pagecontent p {
 
-
text-indent: 1.25em;
 
-
}
 
-
p.start:first-line {
+
<font color="#000000" face="arial" size="1" >
-
font-weight: bold;
+
<p align="justify">
-
letter-spacing: 1.2;
+
As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi" ><i>Aspergillus nidulans</i></a> and the <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/mammalian"> mammalian cell line U-2 OS </a> with great success.</p>
-
}
+
</font>
 +
</div>
 +
<div class="blue">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>Flexibility</b><br><br>
 +
</font>
 +
<div class="image2">
-
</style>
 
-
<! -- END of style sheet -->
 
-
</head>
+
<img src="https://static.igem.org/mediawiki/2011/a/aa/Plasmid_puzzle_colour.jpg" width="187px" height="50px"> </img><br>
-
<body>
+
 +
</div>
-
<!-- Table displaying top row of links -->
 
-
<table class="links" >
 
-
  <tr>
 
-
    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2011.igem.org/Team:DTU-Denmark-2" >Home</a></font> </td>
 
-
    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2011.igem.org/Team:DTU-Denmark-2/Team" >The Team</a> </font></td>
 
-
    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2011.igem.org/Team:DTU-Denmark-2/Project" >The Project</a> </font></td>
 
-
      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2011.igem.org/Team:DTU-Denmark-2/Safety">Safety</a></font> </td>
 
-
      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2011.igem.org/Team:DTU-Denmark-2/Notebook">Notebook</a></font> </td>
 
-
      <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://www.facebook.com/pages/DTU-iGEM-Team-2011/217344154973456">FACEBOOK</a></font> </td>
 
-
</font></td>
 
-
  </tr>
 
-
</table>
 
-
<table> <!-- Table for content area -->
 
-
<!-- Main content area -->
+
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
Standardization entails rigidity. Therefore we have written a <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization" >guide</a> that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.</p>
 +
</font>
 +
</div>
 +
 
 +
<div class="yellow">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>Applications</b><br><br>
 +
</font>
 +
<div class="image2">
 +
<img src="https://static.igem.org/mediawiki/2011/8/8f/Application.jpg" width="187px" height="50px"> </img><br>
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/introduction#Application%20area">Here</a> you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.</p>
 +
</font>
 +
</div>
-
<table cellpadding=10px>
 
-
<tr>
 
-
  <td width="163px" height="100%" valign="top">
 
-
  <font color="#990000" face="arial" size="3">
 
-
  <br>
 
-
    Meet the team<br><br>
 
-
  <font color="DDDDDD" face="arial" size="2">
 
-
<ul>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Team/Supervisors" CLASS=leftbar>Supervisors</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Team/Advisors" CLASS=leftbar>Advisors</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Team/Students" CLASS=leftbar>Students</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Team/Environment" CLASS=leftbar>Environment</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Team/Gallery" CLASS=leftbar>Gallery</a></li>
 
-
    </ul>
 
-
  </font>
 
-
<br>
 
-
<font color="#990000" face="arial" size="3">
 
-
<br>   
 
-
Plug 'n' PLay
 
-
  </font>
 
-
  <font color="DDDDDD" face="arial" size="2">
 
-
<ul>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/Protocol" CLASS=leftbar>Protocol</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/BioBricks#USER" CLASS=leftbar>Submitted parts</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Results#USER" CLASS=leftbar>Results</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/Future" CLASS=leftbar>Future</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark/Project/References" CLASS=leftbar>References</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark/Aspergillus" CLASS=leftbar>References</a></li>
 
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Mammalian%20cells" CLASS=leftbar>Mammalian cells</a></li>
 
-
</ul>
 
-
 
 
-
  </font>
 
<br>
<br>
-
<font color="#990000" face="arial" size="3">
+
<br>
-
<br>  
+
<div class="purple">
-
Notebook
+
<font color="#000000" face="arial" size="3" >
 +
<b>Data Page</b><br><br>
 +
</font>
 +
 
 +
<div class="image2">
 +
 
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/c/c3/AspergillusDTU_billed.jpg" width="187px" height="50px"> </img><br>
-
  </font>
+
</div>
-
  <font color="DDDDDD" face="arial" size="2">
+
-
<ul>
+
-
    <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Notebook" CLASS=leftbar>Notebook</a></li>
+
-
  </font>
+
<font color="#000000" face="arial" size="1" >
-
  </td>
+
-
  <td width="556px" height="100%" valign="top">
+
-
  <font color="#990000" face="arial" size="5">
+
-
<br> 
+
-
<b>The 2011 Technical University of Denmark iGEM team Plug n’ Play</b><br><br>
+
-
  </font>
+
<p align="justify">
<p align="justify">
-
The emerging and fast growing field of molecular and synthetic biology calls for simpler, faster and more efficient cloning techniques. Conventional cloning techniques include restriction digestion and ligation, which can be both time consuming and complex – especially when working with mammalian cells and fungi.
+
You can find an overview of our project on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/data_page">Data Page</a>. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.</p>
-
So in order to optimize the cloning process to become faster and more efficient, it would be convenient to avoid the use of restriction enzymes.
+
</font>
 +
</div>
-
In early 1990s, the uracil excision-based cloning was invented as a ligation-independent cloning technique that could substitute the conventional cloning with restriction enzymes and ligase. This technique was further developed, and a few years later, New England Biolabs (NEB) introduced the USERTM  Friendly Cloning Kit, which was to ensure cloning without the use of restriction enzymes. However, the kit was not compatible with proofreading polymerases, since they stalled when encountering a uracil base in the DNA template, as it is a promutanegic event (Nour-Eldin et al., 2010; New England Biolabs, 2004).
+
<div class="lightgreen">
-
However, newer proofreading polymerases have been developed that can read through the uracils and thus be compatible with the concept of USER. This has opened the door to a more simple and flexible approach to molecular cloning techniques.
+
<font color="#000000" face="arial" size="3" >
-
The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs can anneal each other to form a stable hybridization product. The overhangs on the PCR product are custom made and independent of restriction sites, which is very convenient when working with fungi and mammalian cells.
+
<b>Achievements</b><br><br>
 +
</font>
 +
<div class="image2">
-
We will develop a standardized cloning system, called “Plug’n’Play”, where certain categories of biological parts can be gathered. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of PCR-products will be distributed in microtiter plates directly ready for cloning. Furthermore, the “Plug’n’Play” kit will contain a back-up where all parts are contained on a plasmid to ensure amplification of a mutation free template if needed. 
 
-
The simple and easy use of the system will be demonstrated by developing a reporter targeting system for the fungus Aspergillus niger. Furthermore, will we demonstrate its application in mammalian cells."</p>
 
 +
<img src="https://static.igem.org/mediawiki/2011/f/f7/Achievements.jpg" width="187px" height="50px"> </img><br>
-
  <td width="182px" height="100%" valign="top" class="greybox">
+
</div>
-
  <font face="arial" size="2">
+
 
 +
 
 +
 
 +
 
 +
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
Designing a novel assembly standard and creating a reporter system for <i>Aspergillus nidulans</i> and mammalian cells are our main achievements. You can read more about our accomplishments on <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievements"> this page</a>.</p>
 +
</font>
 +
</div>
 +
 
 +
<div class="pink">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>The Team</b><br><br>
 +
</font>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/thumb/f/fd/DSC_0349.jpg/800px-DSC_0349.jpg" width="187px" height="50px"> </img><br>
 +
 
 +
 
 +
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/Team">Team Page</a>.</p>
 +
</font>
 +
</div>
 +
 
 +
  <td width="182px" height="100%" valign="top" >
 +
<div class="grey">
 +
<br>
 +
 
 +
<div class="image">
 +
 
 +
<font color="#990000" face="arial" size="3" >
 +
 
 +
<a href="https://igem.org/Team_Wikis?year=2011"><b>iGEM 2011 Wiki Main Page</b></a>
 +
 
 +
<a href="https://2011.igem.org/Main_Page" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/3/3f/Igem.png
 +
" height="120px" align="Center">  </a>
 +
</font>
 +
</div>
-
<!-- INSERT GREY BOX TEXT HERE! (formatting: <b>bold</> <i>italic> <h4>header</h4>) -->
 
-
<p>
 
<br>
<br>
-
<font size="3"><strong>Quick Update</strong></font><br>
 
<br>
<br>
-
   </font>
+
<div id="fb-root"></div>
 +
<script>(function(d, s, id) {
 +
   var js, fjs = d.getElementsByTagName(s)[0];
 +
  if (d.getElementById(id)) {return;}
 +
  js = d.createElement(s); js.id = id;
 +
  js.src = "//connect.facebook.net/en_US/all.js#xfbml=1";
 +
  fjs.parentNode.insertBefore(js, fjs);
 +
}(document, 'script', 'facebook-jssdk'));</script>
 +
 
 +
<div class="fb-like-box" data-href="https://www.facebook.com/pages/DTU-iGEM-Team-2011/217344154973456" data-width="250" data-show-faces="true" data-stream="true" data-header="true"></div>
 +
<br>
 +
<br>
 +
<br>
 +
<div class="slogan">
 +
<span id="slogan"></span>
 +
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js" charset="utf-8"></script>
 +
<script type="text/javascript" charset="utf-8">
 +
$(function(){
 +
  var slogan = new Array();
 +
 
 +
  slogan[0] = "It’s fast, it’s furious, it’s Plug’n’Play with DNA";
 +
  slogan[1] = "Pack your ligases and restriction enzymes away Plug’n’play with DNA is here to stay";
 +
  slogan[2] = "Take action and improve your reaction";
 +
  slogan[3] = "Making research easy and without sorrow, our Plug’n’play kit you can borrow";
 +
  slogan[4] = "Making research easy and without tears, with our Plug’n’play kit you can save years";
 +
  slogan[5] = "Dry your tears and save some years Plug’n’play Is here to stay";
 +
  slogan[6] = "Use Plug’n’play and reasearch will be a joy every day";
 +
  slogan[7] = "Plug’n’play with DNA, an easy way to save your day";
 +
  slogan[8] = "Plug’n’play with DNA, it’s like Speedy Gonzales in microtiter plates";
 +
  slogan[9] = "Plug’n’play with DNA will change the world today";
 +
  slogan[10] = "Plug’n’play can hold your favorite DNA";
 +
  slogan[11] = "Mix it, heat it, use it, it's that easy";
 +
  slogan[12] = "Avoid restriction, use the plug 'n' play edition";
 +
  slogan[13] = "Make a smart decision, use the plug 'n' play edition";
 +
  slogan[14] = "Plug’n’play with DNA is complete, it is easy, cool and smart, indeed";
 +
 
 +
  var rand = Math.floor(Math.random()*slogan.length);
 +
  $('#slogan').text("\"" + slogan[rand] + "\"");
 +
});
 +
</script>
 +
</div>
 +
 
 +
 
 +
</div>
 +
<br>
 +
<br>
 +
 
  </td>
  </td>
</table>
</table>
-
 
+
<br>
 +
<br>
<table>
<table>
 +
<tr height="25px">
<tr height="25px">
-
<td width="967px" valign="center" align="center">
+
<td width="976px" valign="center" align="center">
 +
  <font color="#000000" face="arial" size="5">
 +
<b> Sponsored by</b><br><br>
 +
</font>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/a/a2/Novozymes.jpeg" with="150px"height="150px"> </img>&nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/5/5c/Bn_logo_rgb.jpg" with="260px"height="260px"> </img> <br><br>
 +
<img src="https://static.igem.org/mediawiki/2009/d/de/Ottomfond.jpg" with="230px"height="65px"> </img>&nbsp;
 +
<img src="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif"height="40px"> </img> <br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/0/0c/Alk_Logo.jpg" with="80px"height="80px"> </img> &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/7/74/Novo_nordisk_logo.jpg" with="150px"height="150px"> </img> &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/e/e0/DNA_technology_logo.png" with="100px"height="100px"> </img>
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2011/2/21/IDTLogo2010.png"with="40px"height="40px"> </img> &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/9/90/Pharmadanmark.png"with="50px"height="50px"> </img> &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/0/01/Cmb.jpg" with="70px"height="70px"> </img>  &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/2/22/Dtu-uk-a2-200.png" with="50px"height="50px" > </img> &nbsp;
-
<font face="arial" size="1">
+
<br> 
 +
<br> 
 +
<font face="arial" size="1"align="center">
  Comments or questions to the team? Please <a href="mailto:DTUpowerpuff@googlegroups.com" CLASS=email>Email us</a>
  Comments or questions to the team? Please <a href="mailto:DTUpowerpuff@googlegroups.com" CLASS=email>Email us</a>
</font>
</font>

Latest revision as of 23:10, 21 September 2011


Making Molecular Biology Easier


We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and Aspergilli ready to plug 'n' play. You can find more information about how this standard works here.


A Reporter System

As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism Aspergillus nidulans and the mammalian cell line U-2 OS with great success.
Flexibility

Standardization entails rigidity. Therefore we have written a guide that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.
Applications

Here you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.


Data Page

You can find an overview of our project on our Data Page. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.
Achievements

Designing a novel assembly standard and creating a reporter system for Aspergillus nidulans and mammalian cells are our main achievements. You can read more about our accomplishments on this page.
The Team

We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our Team Page.

iGEM 2011 Wiki Main Page









Sponsored by

  

 

   

       

Comments or questions to the team? Please

Retrieved from "http://2011.igem.org/Team:DTU-Denmark-2"