Team:Paris Liliane Bettencourt/Notebook/2011/07/12/
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(Created page with "Kevin '''Plasmid cultures from David Lane (LacO/tetO array)''' -10mL LB + 10μL Antibiotics overnight -pDAG480 -> CmR - 1 -pDAG479 -> CmR - 2 -p...") |
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- | + | {{:Team:Paris_Bettencourt/tpl_test}} | |
- | + | ==Kevin== | |
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- | -pDAG480 -> CmR - 1 | + | '''Plasmid cultures from David Lane (LacO/tetO array)''' <br> |
- | -pDAG479 -> CmR - 2 | + | -10mL LB + 10μL Antibiotics overnight<br><br> |
- | -pFX234 -> Amp - 3 | + | |
- | -pDAG464 -> CmR , KanR - 4 | + | -pDAG480 -> CmR - 1 - tetO array<br> |
- | -pSG20 -> Amp - 5 | + | -pDAG479 -> CmR - 2 - tetO array<br> |
+ | -pFX234 -> Amp - 3 - yfp:tetR<br> | ||
+ | -pDAG464 -> CmR , KanR - 4 - lacO array<br> | ||
+ | -pSG20 -> Amp - 5 - gfp:lacI<br> | ||
+ | |||
+ | ==Mathias & Laura== | ||
+ | |||
+ | After sequencing, we could see the BioBrick the parts registry sent us under the name K143055 was not pHyperSpank + SpoVg but pVeg + SpoVg we have to all redo. |
Latest revision as of 15:53, 21 September 2011
Kevin
Plasmid cultures from David Lane (LacO/tetO array)
-10mL LB + 10μL Antibiotics overnight
-pDAG480 -> CmR - 1 - tetO array
-pDAG479 -> CmR - 2 - tetO array
-pFX234 -> Amp - 3 - yfp:tetR
-pDAG464 -> CmR , KanR - 4 - lacO array
-pSG20 -> Amp - 5 - gfp:lacI
Mathias & Laura
After sequencing, we could see the BioBrick the parts registry sent us under the name K143055 was not pHyperSpank + SpoVg but pVeg + SpoVg we have to all redo.