Team:DTU-Denmark-2

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<b>Making Molecular Biology Easier</b>
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<b>We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and <i>Aspergilli </i> ready to plug 'n' play. You can find more information about how this standard works <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly">here</a>.<b>
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<b>A Reporter System</b><br><br>
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<div class="image2">
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<li><a href="https://2011.igem.org/Team:DTU-Denmark-2" class="dir">home</a>
 
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<li><a href="https://2010.igem.org/Team:DTU-Denmark-2/Team" class="dir">Meet the team</a>
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<img src="https://static.igem.org/mediawiki/2011/8/84/IGEM_001.jpg" width="187px" height="50px"> </img><br>
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  <li><a href="https://igem.org/Team.cgi?id=322">official</a></li>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Team/Supervisors">supervisors</a></li>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Team/Advisors">advisors</a></li>
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  <li><a href="https://2010.igem.org/Team:DTU-Denmark-2/Team/Students">students</a></li>
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  <li><a href="https://2010.igem.org/Team:DTU-Denmark-2/Team/Environment">environment</a></li>
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<li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Project" class="dir">Plug n’ Play</a>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/Protocol">the protocol</a></li>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/BioBricks#USER">submitted parts</a></li>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Results#USER">results</a></li>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/Future">the future</a></li>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark/Project/References">references</a></li>
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<li><a href="https://2011.igem.org/Team:DTU-Denmark/Aspergillus" class="dir">Aspergillus nidulans</a>
 
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  <ul>
 
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Aspergillus/Aspergillus niger">the project</a></li>
 
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<li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Mammalian cells" class="dir">Mammalian</a>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/HEK293/HEK">HEK2</a></li>
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As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi" ><i>Aspergillus nidulans</i></a> and the <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/mammalian"> mammalian cell line U-2 OS </a> with great success.</p>
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<li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Safety" class="dir">Safety issues</a>
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Safety/Safety proposal">communication of safety</a></li>
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<b>Flexibility</b><br><br>
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<li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Notebook" class="dir">lab notes&nbsp;&nbsp;&nbsp;</a>
 
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Notebook/Collaboration">collaboration</a></li>
 
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Notebook/Attribution">attribution</a></li>
 
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Notebook/Aspergillus">Aspergillus nidulans</a></li>
 
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  <li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Notebook/Mammalian cells">HEK293</a></li>
 
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<li><a href="https://2011.igem.org/Team:DTU-Denmark-2/Acknowledgements" class="dir">acknowledgements</a>
 
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<img src="https://static.igem.org/mediawiki/2011/a/aa/Plasmid_puzzle_colour.jpg" width="187px" height="50px"> </img><br>
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Standardization entails rigidity. Therefore we have written a <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization" >guide</a> that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.</p>
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<b>Applications</b><br><br>
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/introduction#Application%20area">Here</a> you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.</p>
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<b>Data Page</b><br><br>
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<img src="https://static.igem.org/mediawiki/2011/c/c3/AspergillusDTU_billed.jpg" width="187px" height="50px"> </img><br>
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You can find an overview of our project on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/data_page">Data Page</a>. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.</p>
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<b>Achievements</b><br><br>
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<img src="https://static.igem.org/mediawiki/2011/f/f7/Achievements.jpg" width="187px" height="50px"> </img><br>
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Designing a novel assembly standard and creating a reporter system for <i>Aspergillus nidulans</i> and mammalian cells are our main achievements. You can read more about our accomplishments on <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievements"> this page</a>.</p>
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<b>The Team</b><br><br>
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<img src="https://static.igem.org/mediawiki/2011/thumb/f/fd/DSC_0349.jpg/800px-DSC_0349.jpg" width="187px" height="50px"> </img><br>
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We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/Team">Team Page</a>.</p>
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<a href="https://igem.org/Team_Wikis?year=2011"><b>iGEM 2011 Wiki Main Page</b></a>
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<a href="https://2011.igem.org/Main_Page" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/3/3f/Igem.png
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<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js" charset="utf-8"></script>
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<b>The 2010 <a class="headlink" href="http://www.bio.dtu.dk">The Technical University of Denmark</a> iGEM <a class="headlink" href="https://2011.igem.org/Team:DTU-Denmark-2/Team">team</a> Plug n’ Play
+
  slogan[0] = "It’s fast, it’s furious, it’s Plug’n’Play with DNA";
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  slogan[1] = "Pack your ligases and restriction enzymes away Plug’n’play with DNA is here to stay";
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  slogan[2] = "Take action and improve your reaction";
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  slogan[3] = "Making research easy and without sorrow, our Plug’n’play kit you can borrow";
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  slogan[4] = "Making research easy and without tears, with our Plug’n’play kit you can save years";
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  slogan[5] = "Dry your tears and save some years Plug’n’play Is here to stay";
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  slogan[6] = "Use Plug’n’play and reasearch will be a joy every day";
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  slogan[7] = "Plug’n’play with DNA, an easy way to save your day";
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  slogan[8] = "Plug’n’play with DNA, it’s like Speedy Gonzales in microtiter plates";
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  slogan[9] = "Plug’n’play with DNA will change the world today";
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  slogan[10] = "Plug’n’play can hold your favorite DNA";
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  slogan[11] = "Mix it, heat it, use it, it's that easy";
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  slogan[12] = "Avoid restriction, use the plug 'n' play edition";
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  slogan[13] = "Make a smart decision, use the plug 'n' play edition";
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  slogan[14] = "Plug’n’play with DNA is complete, it is easy, cool and smart, indeed";
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<li>A structured framework for non-chemical <a class="headlink" href="https://2011.igem.org/Team:DTU-Denmark-2/Bacterial/Core_repressilator">communication</a> between <i>E. coli</i>, codenamed <a class="headlink" href="https://2011.igem.org/Team:DTU-Denmark-2/Bacterial">FORTH</a>, pairing light-producing and light-sensing BioBricks such that future projects can be developed for a variety of novel applications.
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<b> Sponsored by</b><br><br>
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</font>
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<img src="https://static.igem.org/mediawiki/2011/a/a2/Novozymes.jpeg" with="150px"height="150px"> </img>&nbsp;
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<img src="https://static.igem.org/mediawiki/2011/5/5c/Bn_logo_rgb.jpg" with="260px"height="260px"> </img> <br><br>
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<img src="https://static.igem.org/mediawiki/2009/d/de/Ottomfond.jpg" with="230px"height="65px"> </img>&nbsp;
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<img src="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif"height="40px"> </img> <br><br>
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<img src="https://static.igem.org/mediawiki/2011/0/0c/Alk_Logo.jpg" with="80px"height="80px"> </img> &nbsp;
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<img src="https://static.igem.org/mediawiki/2011/7/74/Novo_nordisk_logo.jpg" with="150px"height="150px"> </img> &nbsp;
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<img src="https://static.igem.org/mediawiki/2011/e/e0/DNA_technology_logo.png" with="100px"height="100px"> </img>  
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<p>" The use of restriction digestion and ligation although developed over 20 years ago, remains to be the standard cloning technique. The use of restriction enzymes can be both a time consuming and cumbersome process. To optimize the cloning process to become faster and more efficient, it is desirable to avoid the use of restriction enzymes.
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  Back in 2003 the USER friendly cloning was developed by New England Biolabs to ensure cloning without the use of restriction enzymes. Further development of the method in recent years has meant that construction of the vectors used for USER cloning can now be made quickly and efficiently without the use of restriction enzymes.  
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Comments or questions to the team? Please <a href="mailto:DTUpowerpuff@googlegroups.com" CLASS=email>Email us</a>
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We are developing a standardized system, where certain categories of biological parts can be gathered. The idea is that the parts are in microtiter plates where they can be directly mixed with other desired parts and an expression vector and then can be assembled within a few hours. Hereby purification of digested vectors and PCR-products and ligation are avoided. To show how easy it is, we will demonstrate the use of this system by developing a reporter targeting system for the fungus Aspergillus niger and furthermore demonstrate its application in mammalian cells." - <a
 
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Latest revision as of 23:10, 21 September 2011


Making Molecular Biology Easier


We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and Aspergilli ready to plug 'n' play. You can find more information about how this standard works here.


A Reporter System

As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism Aspergillus nidulans and the mammalian cell line U-2 OS with great success.
Flexibility

Standardization entails rigidity. Therefore we have written a guide that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.
Applications

Here you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.


Data Page

You can find an overview of our project on our Data Page. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.
Achievements

Designing a novel assembly standard and creating a reporter system for Aspergillus nidulans and mammalian cells are our main achievements. You can read more about our accomplishments on this page.
The Team

We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our Team Page.

iGEM 2011 Wiki Main Page









Sponsored by

  

 

   

       

Comments or questions to the team? Please

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