Team:DTU-Denmark-2

From 2011.igem.org

(Difference between revisions)
 
(202 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
{{:Team:DTU-Denmark-2/Header}}
-
 
+
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
 
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
<head>
-
This is a template page. READ THESE INSTRUCTIONS.
+
<style type="text/css">
 +
 
 +
 
 +
 
 +
div.flash
 +
{
 +
display: block;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
left:0px;
 +
top:0px;
 +
z-index:-200;
 +
}
 +
ul.dropdown * {
 +
        z-index: 200;
 +
}
 +
 
 +
  div.image {
 +
    padding-right: 10px;  
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    text-align:center;
 +
  }
 +
div.image2 {
 +
   
 +
    display: block;
 +
    margin-left: auto;
 +
    margin-right: auto;
 +
  }
 +
 
 +
  div.slogan {
 +
  padding-right: 20px;
 +
    padding-left: 20px;
 +
    padding-bottom:20px;
 +
    padding-top:20px;
 +
    background-color:#FBFF74;
 +
    border-style: bold;
 +
    border-width: 1px;
 +
    border-color:#F2F2F2;
 +
    -webkit-box-shadow: 5px 5px 2px #888888; /* Safari */
 +
    box-shadow: 5px 5px 2px #888888;
 +
    font-style:italic;
 +
    font-weight:bold;
 +
    font-size:120%;
 +
    font-family:"arial";
 +
 
 +
}
 +
div.igemlogo img {
 +
    margin: 20px 20px 20px 20px;
 +
        }
 +
div.red {
 +
    padding-right: 30px;
 +
    padding-left: 30px;
 +
    padding-bottom:30px;
 +
    padding-top:30px;
 +
    text-align:center;
 +
    background-color:#E7F9FF
 +
}
 +
div.grey {
 +
 
 +
    padding-right: 5px;  
 +
    padding-left: 5px;
 +
    padding-bottom:5px;
 +
    padding-top:5px;
 +
    width: 260px;
 +
    height: 1150px;
 +
    background-color:#F9F9F9
 +
  }
 +
 +
div.green {
 +
    padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width: 189px;
 +
    height: 220px;
 +
    background-color:#D8F6CE;
 +
    float:left;
 +
    margin:5px;
 +
    line-height:120%;
 +
}
 +
div.blue {
 +
    padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width: 189px;
 +
    height: 220px;
 +
    line-height:120%;
 +
      background-color:#FFDEB6;
 +
float:left;
 +
margin:5px;
 +
}
 +
div.yellow {
 +
    padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width: 189px;
 +
    height: 220px;
 +
    float:left;
 +
    margin:5px;
 +
line-height:120%;
 +
    background-color:#F6CED8;
 +
}
 +
div.purple {
 +
  padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width:  189px;
 +
    height:  220px;
 +
    float:left;
 +
    margin:5px;
 +
    line-height:120%;
 +
    background-color:#A9E2F3;
 +
}
 +
div.lightgreen {
 +
  padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width:  189px;
 +
    height:  220px;
 +
    line-height:120%;
 +
float:left;
 +
    margin:5px;
 +
   
 +
background-color:#FAFEBA;
 +
}
 +
div.pink {
 +
    padding-right: 10px;
 +
    padding-left: 10px;
 +
    padding-bottom:10px;
 +
    padding-top:10px;
 +
    width:  189px;
 +
    height:  220px;
 +
    line-height:120%;
 +
float:left;
 +
    margin:5px;
 +
    background-color:#CEF6E3;
 +
}
 +
<!--
 +
A{text-decoration:none}
 +
A:link {color:  #0000FF;text-decoration: none }
 +
A:active {color:  #0000FF;text-decoration: none }
 +
A:visited {color:  #0000FF ;text-decoration: none }
 +
A:hover {text-decoration: none}
 +
-->
 +
 
 +
</style>
 +
</head>
 +
 
 +
<body>
 +
 
 +
 
 +
<table> <!-- Table for content area -->
 +
 
 +
 
 +
<!-- Main content area -->
 +
<table cellpadding=10px>
 +
 
 +
<td width="660px" height="100%" valign="top" >
 +
 
 +
<div class="flash">
 +
<object type="application/x-shockwave-flash" width="600" height="400" data="https://static.igem.org/mediawiki/2011/2/2b/Flash-anim-4_hurtig.swf">
 +
<param name="movie" value="https://static.igem.org/mediawiki/2011/2/2b/Flash-anim-4_hurtig.swf")>
 +
<param name="wmode" value="transparent">
 +
</object>
 +
 
</div>
</div>
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
 
-
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
+
<div class="red">
 +
 
 +
<p align="justify">
 +
<font color="#000000" face="arial" size="5" >
 +
<b>Making Molecular Biology Easier</b>
 +
</font></p>
 +
<font color="#000000" face="arial" size="2" >
 +
<p align="justify">
 +
<br>
 +
<p align="justify">
 +
<b>We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and <i>Aspergilli </i> ready to plug 'n' play. You can find more information about how this standard works <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly">here</a>.<b>
 +
</p>
 +
</font>
</div>
</div>
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
<br>
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
+
 
 +
<div class="green">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>A Reporter System</b><br><br>
 +
</font>
 +
<div class="image2">
 +
 
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/8/84/IGEM_001.jpg" width="187px" height="50px"> </img><br>
 +
 
</div>
</div>
 +
 +
 +
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi" ><i>Aspergillus nidulans</i></a> and the <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/mammalian"> mammalian cell line U-2 OS </a> with great success.</p>
 +
</font>
</div>
</div>
-
</html>
 
-
<!-- *** End of the alert box *** -->
+
<div class="blue">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>Flexibility</b><br><br>
 +
</font>
 +
<div class="image2">
-
[[File:Logoigempeginav1.png‎]]
 
-
{|align="justify"
+
<img src="https://static.igem.org/mediawiki/2011/a/aa/Plasmid_puzzle_colour.jpg" width="187px" height="50px"> </img><br>
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
-
|[[Image:DTU-Denmark-2_logo.png|200px|right|frame]]
+
-
|-
+
-
|
+
-
''Tell us more about your project. Give us background. Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
-
|[[Image:DTU-Denmark-2_team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:DTU-Denmark-2 | Team Example]]
+
-
|}
+
-
<!--- The Mission, Experiments --->
+
</div>
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
 
-
!align="center"|[[Team:DTU-Denmark-2|Home]]
+
 
-
!align="center"|[[Team:DTU-Denmark-2/Team|Team]]
+
 
-
!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=DTU-Denmark-2 Official Team Profile]
+
 
-
!align="center"|[[Team:DTU-Denmark-2/Project|Project]]
+
<font color="#000000" face="arial" size="1" >
-
!align="center"|[[Team:DTU-Denmark-2/Parts|Parts Submitted to the Registry]]
+
<p align="justify">
-
!align="center"|[[Team:DTU-Denmark-2/Modeling|Modeling]]
+
Standardization entails rigidity. Therefore we have written a <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization" >guide</a> that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.</p>
-
!align="center"|[[Team:DTU-Denmark-2/Notebook|Notebook]]
+
</font>
-
!align="center"|[[Team:DTU-Denmark-2/Safety|Safety]]
+
</div>
-
!align="center"|[[Team:DTU-Denmark-2/Attributions|Attributions]]
+
 
-
|}
+
<div class="yellow">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>Applications</b><br><br>
 +
</font>
 +
<div class="image2">
 +
<img src="https://static.igem.org/mediawiki/2011/8/8f/Application.jpg" width="187px" height="50px"> </img><br>
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/introduction#Application%20area">Here</a> you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.</p>
 +
</font>
 +
</div>
 +
 
 +
 
 +
<br>
 +
<br>
 +
<div class="purple">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>Data Page</b><br><br>
 +
</font>
 +
 
 +
<div class="image2">
 +
 
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/c/c3/AspergillusDTU_billed.jpg" width="187px" height="50px"> </img><br>
 +
 
 +
</div>
 +
 
 +
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
You can find an overview of our project on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/data_page">Data Page</a>. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.</p>
 +
</font>
 +
</div>
 +
 
 +
<div class="lightgreen">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>Achievements</b><br><br>
 +
</font>
 +
<div class="image2">
 +
 
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/f/f7/Achievements.jpg" width="187px" height="50px"> </img><br>
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
Designing a novel assembly standard and creating a reporter system for <i>Aspergillus nidulans</i> and mammalian cells are our main achievements. You can read more about our accomplishments on <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievements"> this page</a>.</p>
 +
</font>
 +
</div>
 +
 
 +
<div class="pink">
 +
<font color="#000000" face="arial" size="3" >
 +
<b>The Team</b><br><br>
 +
</font>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/thumb/f/fd/DSC_0349.jpg/800px-DSC_0349.jpg" width="187px" height="50px"> </img><br>
 +
 
 +
 
 +
<font color="#000000" face="arial" size="1" >
 +
<p align="justify">
 +
We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/Team">Team Page</a>.</p>
 +
</font>
 +
</div>
 +
 
 +
<td width="182px" height="100%" valign="top" >
 +
<div class="grey">
 +
<br>
 +
 
 +
<div class="image">
 +
 
 +
<font color="#990000" face="arial" size="3" >
 +
 
 +
<a href="https://igem.org/Team_Wikis?year=2011"><b>iGEM 2011 Wiki Main Page</b></a>
 +
 
 +
<a href="https://2011.igem.org/Main_Page" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/3/3f/Igem.png
 +
" height="120px" align="Center">  </a>
 +
</font>
 +
</div>
 +
 
 +
 
 +
<br>
 +
<br>
 +
 
 +
<div id="fb-root"></div>
 +
<script>(function(d, s, id) {
 +
  var js, fjs = d.getElementsByTagName(s)[0];
 +
  if (d.getElementById(id)) {return;}
 +
  js = d.createElement(s); js.id = id;
 +
  js.src = "//connect.facebook.net/en_US/all.js#xfbml=1";
 +
  fjs.parentNode.insertBefore(js, fjs);
 +
}(document, 'script', 'facebook-jssdk'));</script>
 +
 
 +
<div class="fb-like-box" data-href="https://www.facebook.com/pages/DTU-iGEM-Team-2011/217344154973456" data-width="250" data-show-faces="true" data-stream="true" data-header="true"></div>
 +
<br>
 +
<br>
 +
<br>
 +
<div class="slogan">
 +
<span id="slogan"></span>
 +
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js" charset="utf-8"></script>
 +
<script type="text/javascript" charset="utf-8">
 +
$(function(){
 +
  var slogan = new Array();
 +
 
 +
  slogan[0] = "It’s fast, it’s furious, it’s Plug’n’Play with DNA";
 +
  slogan[1] = "Pack your ligases and restriction enzymes away Plug’n’play with DNA is here to stay";
 +
  slogan[2] = "Take action and improve your reaction";
 +
  slogan[3] = "Making research easy and without sorrow, our Plug’n’play kit you can borrow";
 +
  slogan[4] = "Making research easy and without tears, with our Plug’n’play kit you can save years";
 +
  slogan[5] = "Dry your tears and save some years Plug’n’play Is here to stay";
 +
  slogan[6] = "Use Plug’n’play and reasearch will be a joy every day";
 +
  slogan[7] = "Plug’n’play with DNA, an easy way to save your day";
 +
  slogan[8] = "Plug’n’play with DNA, it’s like Speedy Gonzales in microtiter plates";
 +
  slogan[9] = "Plug’n’play with DNA will change the world today";
 +
  slogan[10] = "Plug’n’play can hold your favorite DNA";
 +
  slogan[11] = "Mix it, heat it, use it, it's that easy";
 +
  slogan[12] = "Avoid restriction, use the plug 'n' play edition";
 +
  slogan[13] = "Make a smart decision, use the plug 'n' play edition";
 +
  slogan[14] = "Plug’n’play with DNA is complete, it is easy, cool and smart, indeed";
 +
 
 +
  var rand = Math.floor(Math.random()*slogan.length);
 +
  $('#slogan').text("\"" + slogan[rand] + "\"");
 +
});
 +
</script>
 +
</div>
 +
 
 +
 
 +
</div>
 +
<br>
 +
<br>
 +
 
 +
</td>
 +
</table>
 +
<br>
 +
<br>
 +
<table>
 +
 
 +
 
 +
<tr height="25px">
 +
<td width="976px" valign="center" align="center">
 +
  <font color="#000000" face="arial" size="5">
 +
<b> Sponsored by</b><br><br>
 +
</font>
 +
 
 +
<img src="https://static.igem.org/mediawiki/2011/a/a2/Novozymes.jpeg" with="150px"height="150px"> </img>&nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/5/5c/Bn_logo_rgb.jpg" with="260px"height="260px"> </img> <br><br>
 +
<img src="https://static.igem.org/mediawiki/2009/d/de/Ottomfond.jpg" with="230px"height="65px"> </img>&nbsp;
 +
<img src="https://static.igem.org/mediawiki/2010/4/47/Lundbeckfonden.gif"height="40px"> </img> <br><br>
 +
<img src="https://static.igem.org/mediawiki/2011/0/0c/Alk_Logo.jpg" with="80px"height="80px"> </img> &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/7/74/Novo_nordisk_logo.jpg" with="150px"height="150px"> </img> &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/e/e0/DNA_technology_logo.png" with="100px"height="100px"> </img>
 +
<br>
 +
<br>
 +
<img src="https://static.igem.org/mediawiki/2011/2/21/IDTLogo2010.png"with="40px"height="40px"> </img> &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/9/90/Pharmadanmark.png"with="50px"height="50px"> </img> &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/0/01/Cmb.jpg" with="70px"height="70px"> </img>  &nbsp;
 +
<img src="https://static.igem.org/mediawiki/2011/2/22/Dtu-uk-a2-200.png" with="50px"height="50px" > </img> &nbsp;
 +
 
 +
<br> 
 +
<br> 
 +
<font face="arial" size="1"align="center">
 +
Comments or questions to the team? Please <a href="mailto:DTUpowerpuff@googlegroups.com" CLASS=email>Email us</a>
 +
</font>
 +
 
 +
 
 +
</td>
 +
</tr>
 +
 
 +
</table>
 +
 
 +
</body>
 +
 
 +
</html>

Latest revision as of 23:10, 21 September 2011


Making Molecular Biology Easier


We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and Aspergilli ready to plug 'n' play. You can find more information about how this standard works here.


A Reporter System

As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism Aspergillus nidulans and the mammalian cell line U-2 OS with great success.
Flexibility

Standardization entails rigidity. Therefore we have written a guide that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.
Applications

Here you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.


Data Page

You can find an overview of our project on our Data Page. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.
Achievements

Designing a novel assembly standard and creating a reporter system for Aspergillus nidulans and mammalian cells are our main achievements. You can read more about our accomplishments on this page.
The Team

We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our Team Page.

iGEM 2011 Wiki Main Page









Sponsored by

  

 

   

       

Comments or questions to the team? Please

Retrieved from "http://2011.igem.org/Team:DTU-Denmark-2"