Copenhagen/14 July 2011
From 2011.igem.org
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==Thursday== | ==Thursday== | ||
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+ | Hurra - the two first edition A2 had the mutations. We'll have our second BioBrick very soon. | ||
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+ | And the human cyps have grown marvellous - not like those lazy plant cyps. We are exhousted form making overnight cultures. | ||
+ | ===Lab Work=== | ||
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+ | ====Plant CYPs==== | ||
+ | '''BioBrick A1''' | ||
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+ | * We took the A1's out and put it in and overnight culture | ||
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+ | '''BioBrick A2''' | ||
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+ | * We extended our cyp A2 with prefix and suffix by a [[Team:Copenhagen/Protocol#PCR Reaction|PCR approach]]. | ||
+ | |||
+ | * The DNA obtained was purified by using a [[Team:Copenhagen/Protocol#PCR_purification|PCR purification kit]]. | ||
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+ | '''BioBrick B1''' | ||
+ | |||
+ | * The transformed bacteria had a cold night and needed more time to grow. So they spend the day in the 37 degress room. | ||
+ | |||
+ | ====Human CYPs==== | ||
+ | |||
+ | * Made overnight cultures. | ||
+ | |||
+ | |||
+ | |||
+ | Back to [[Team:Copenhagen/Notebook|Notebook]] |
Latest revision as of 11:34, 14 July 2011
Contents |
Thursday
Hurra - the two first edition A2 had the mutations. We'll have our second BioBrick very soon.
And the human cyps have grown marvellous - not like those lazy plant cyps. We are exhousted form making overnight cultures.
Lab Work
Plant CYPs
BioBrick A1
- We took the A1's out and put it in and overnight culture
BioBrick A2
- We extended our cyp A2 with prefix and suffix by a PCR approach.
- The DNA obtained was purified by using a PCR purification kit.
BioBrick B1
- The transformed bacteria had a cold night and needed more time to grow. So they spend the day in the 37 degress room.
Human CYPs
- Made overnight cultures.
Back to Notebook