Team:NTNU Trondheim

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=Introduction=
=Introduction=
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The Norwegian University of Science and Technology (NTNU) is the first Norwegian university to enter the iGEM competition. We are happy to be able to compete in this cool event, and are really excited to learn more about synthetic biology. Our project idea revolves around using the alarmone ppGpp to detect stress in ''E. coli'', and basically make it turn red with anger (as seen in cartoons).
 
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==Project description==
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iGEM is the premiere international competition in Synthetic Biology for university students, this year with more than 160 teams from all over the world.  For the first time, a Norwegian team is participating! Our team, consisting of students from the Norwegian University of Science and Technology (NTNU) are really excited to be able to compete, and we are very much looking forward to honing our skills in synthetic biology. Our project idea is based on using the alarmone [http://en.wikipedia.org/wiki/Guanosine_pentaphosphate ppGpp] to detect stress in ''E. coli'', and basically make it turn red with anger!
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'''The idea'''
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The stringent response in bacteria is caused by amino-acid starvation, fatty acid limitation, iron limitation, heat shock and other stress conditions. As a response under these conditions, ''in vitro'' studies have suggested that the alarmone guanosine tetraphosphate (ppGpp) increase to modulate transcription to promote survival. The increase in ppGpp levels causes a redirection of transcription so that genes important for survival are favoured at the expense of those required for growth and proliferation [1].
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So, could we use ppGpp as signal molecule to find out when cells are stressed?
 
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'''The solution'''
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Our system will be based on a promoter that is important for regulating growth and proliferation. At the moment we are trying to use the ''rrnB-p1'' promoter, which has been shown in earlier studies to be highly regulated by the ppGpp molecule. Hopefully the promoter will be down regulated enough by increased levels of ppGpp to turn the repressor ''lacI'' it controls completely off. The ''lacI'' represses a second promoter ''lacP'' that induces the production of a red fluorescent protein (mCherry) and turns the cells red. For a detailed overview of our system, see the figure below.
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[[File:System.jpg||Optimal conditions: Under optimal conditions the rrnB P1 promoter promotes transcription of green fluorescent protein (GFP) and lacI. LacI represses the lacI/pL promoter and leads to no further transcription. Green germs.
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Stressful conditions: Under stressful conditions the ppGpp concentration of the cells will increase and lead to repression of the rrnB P1 promoter. The lacI/pL promoter will no longer be repressed and start the transcription of mCherry. Red germs.
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Optimal conditions: Under optimal conditions the ''rrnB P1'' promoter promotes transcription of green fluorescent protein (GFP) and lacI. LacI represses the ''lacI/pL'' promoter and leads to no further transcription. Green germs.
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Stressful conditions: Under stressful conditions the ppGpp concentration of the cells will increase and lead to repression of the ''rrnB P1'' promoter. The ''lacI/pL'' promoter will no longer be repressed and start the transcription of ''mCherry''. Red germs.
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Reference:
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[[http://www.sciencedirect.com/science/article/pii/S0966842X05000788 1]] Magnusson, L. U., A. Farewell, et al. (2005). "ppGpp: a global regulator in Escherichia coli." Trends Microbiol 13(5): 236-242
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Latest revision as of 07:34, 21 September 2011



Introduction

iGEM is the premiere international competition in Synthetic Biology for university students, this year with more than 160 teams from all over the world. For the first time, a Norwegian team is participating! Our team, consisting of students from the Norwegian University of Science and Technology (NTNU) are really excited to be able to compete, and we are very much looking forward to honing our skills in synthetic biology. Our project idea is based on using the alarmone [http://en.wikipedia.org/wiki/Guanosine_pentaphosphate ppGpp] to detect stress in E. coli, and basically make it turn red with anger!


Our Data page
NTNU2011 forside s.png
Judging Criteria
Team registration Check.png
Complete Judging form Check.png
Team Wiki Check.png
Present a poster and a talk at the iGEM Jamboree Check.png
Submitted one well-characterized standard BioBrick ([http://partsregistry.org/Part:BBa_K639003 registry]) Check.png
Demonstrate one new BioBrick of your own design and construction works as expected ([http://partsregistry.org/Part:BBa_K639003 registry]) Check.png
Characterize the operation of one new BioBrick and enter this information in the [http://partsregistry.org/Part:BBa_K639003 registry] Check.png
Improve an existing BioBrick and enter this information in the [http://partsregistry.org/Part:BBa_K639002 registry] Check.png


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