Team:Freiburg/Notebook/11 July

From 2011.igem.org

(Difference between revisions)
(→Commons)
 
(21 intermediate revisions not shown)
Line 1: Line 1:
-
=Commons=
+
{{:Team:Freiburg/Templates/header}}
 +
<html>
 +
<div id="notebook-page-header">
 +
<div id="notebook-back" width="100px" >
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/8_July">Previous entry</a>
 +
</div>
 +
<div id="notebook-title">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 11 July </a>
 +
</div>
 +
<div id="notebook-next">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/12_July">Next entry</a>
 +
</div>
 +
</div>
 +
</html>
 +
 
 +
 
==Miniprep of Promotor-Ribosome-binding-site-constructs==
==Miniprep of Promotor-Ribosome-binding-site-constructs==
 +
 +
'''Investigators: Sophie'''
 +
As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain
As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain
The DNA-concentrations measured were (in ng/µL):
The DNA-concentrations measured were (in ng/µL):
Line 21: Line 39:
PR6a: 45,7
PR6a: 45,7
PR6b: 38,9
PR6b: 38,9
 +
 +
==<span style="color:red;">reg light receptor</span>==
 +
===Test-digest===
 +
 +
'''Investigators: Julia'''
 +
 +
14 samples:
 +
*M30-M32, M 36-M38
 +
*S24-S29
 +
 +
Enzymes used:
 +
*Enzyme 1: EcoRI
 +
*Enzyme 2: PstI
 +
 +
 +
[[File:Gimp_11_07_2011.jpg|none|caption]]
 +
Image of S24-S26: pcyA and S27-S29: cph8
 +
 +
<span style="color:blue;">Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.</span>
 +
 +
==<span style="color:grey;">Precipitator</span>==
 +
===Transformation===
 +
'''Investigators: Rüdiger'''
 +
To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells.
 +
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 00:54, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

Miniprep of Promotor-Ribosome-binding-site-constructs

Investigators: Sophie

As our PR-DNA run out Sophie inoculated LB with bacteria from our Glycerol-stocks and made two minipreps (a and b) per PR-strain The DNA-concentrations measured were (in ng/µL):

PR1a: 89,7 PR1b: 98,6

PR2a: 78,2 PR2b: 52,4

PR3a: 25,7 PR3b: 37,8

PR4a: 45,7 PR4b: 15,7

PR5a: 35,1 PR5b: 55,9

PR6a: 45,7 PR6b: 38,9

reg light receptor

Test-digest

Investigators: Julia

14 samples:

  • M30-M32, M 36-M38
  • S24-S29

Enzymes used:

  • Enzyme 1: EcoRI
  • Enzyme 2: PstI


caption

Image of S24-S26: pcyA and S27-S29: cph8

Comments: No bands for M30-M32 and M36-M38. It should be repeated with a fresh gel.

Precipitator

Transformation

Investigators: Rüdiger To see the expression of the plastic-binding domain (pbd), the GFP-pbd plasmid was transformed in cells.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/11_July"