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Revision as of 15:03, 8 July 2011 (view source) LauradaSilva (Talk | contribs) ← Older edit		Latest revision as of 15:42, 21 September 2011 (view source) LauradaSilva (Talk | contribs)
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-	<p style="display:block;"><font color="red">Laura</font>	+	==Mathias & Laura==
-	</p><p style="display:block;"><u>Digestion</u>	+
-	</p>	+
-	<ul><li>BBa_K143053 cut by SpeI and PstI	+
-	</li><li>BBa_K143055 cut by EcoR1 and XbaI	+
-	</li></ul>	+
-	<p style="display:block;"><u>Ligation</u>	+	===Miniprep===
-	</p>	+
-	<ul><li>BBa_ E1010  (RFP) - BBa_B0015 (vector) 10.9ng/µL	+	-pVeg + SpoVg (K143053)
-	</li><li>BBa_K143033 (LacI) 5.3ng/µL - BBa_B0015 (vector) 10.9ng/µL	+
-	</li><li>BBa_K143021 (RBS SpoVG)  - BBa_K606001 (vector)	+	-LacI + double Terminator
-	</li></ul>	+
		+	===Digestion and gel extraction===
		+
		+	-pVeg + SpoVg by EcoR1 and Spe1
		+
		+	-LacI + Term (new) by EcoR1 and Xba1
		+
		+	Transformation following the ligation pVeg + SpoVg and LacI + Term creating the new BioBrick K606010 in pSB1C3

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Latest revision as of 15:42, 21 September 2011

Mathias & Laura

Miniprep

-pVeg + SpoVg (K143053)

-LacI + double Terminator

Digestion and gel extraction

-pVeg + SpoVg by EcoR1 and Spe1 

-LacI + Term (new) by EcoR1 and Xba1

Transformation following the ligation pVeg + SpoVg and LacI + Term creating the new BioBrick K606010 in pSB1C3