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Team:Freiburg/Notebook/12 June
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| - | == | + | {{:Team:Freiburg/Templates/header}} |
| + | <html> | ||
| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/11_June">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 12 June </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/13_June">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </html> | ||
| - | == | + | ==Picking clones== |
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| + | '''Investigators: Theo, Sophie''' | ||
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| + | <span style="color:blue;">Comments: On plates PR4, PR6, PR8 and PR 9 cells did not grow. More competent cells were plated out again. Succesful growing on plates PR1, PR2, PR3, PR5 and PR7</span> | ||
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| + | Colonies were picked from plates PR1, PR2, PR3 PR5 and PR7. LB/agar (with 0,5microl Chlorampenicol/ml LB) was inoculated with these cells. | ||
| + | Incubation: Overnight at 37C 140RPM (Start: 18.30, End: next day 12:15) | ||
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| + | ==<span style="color:red;">red light receptor</span>== | ||
| + | ===Amplification of the red-light sensor=== | ||
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| + | '''Investigators: Theo, Sophie''' | ||
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| + | amplified the parts for the red light sensor I15008 and I15009 in LB with 1ul/ml Kanamycin , Overnight at 37C 140RPM<br/> | ||
| + | (Start: 18.30, End: 12:15) | ||
| + | for the main receptor ( BBa_I15010) we have no DNA. | ||
| + | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 01:19, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
