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| + | We had a briefing today chaired by James. Key action points of the day were to organize our CIDs, organize who was in charge of each team aspect and discuss the problems that we thought could be solved by Synthetic Biology. Once these problems had been discussed, we each chose one project that another team member came up with to research. We also were introduced to Professor Freemont and Professor Kitney who gave us an insightful talk about what awaits us. A trip to the Royal Society of Science exhibition ended up turning into a lunch in China town (the exhibition actually starts tomorrow) and we talked to Nicola Morgan who is interested in investigating the use of bacteria in making patterns on clothing.<br> |
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| - | <div>- Evidence suggests that several components found in most sunscreens are harmful to us and can be carcinogenic, also most sunscreens only protect against UV B (315-280 nm) and not UV A (<span class="Apple-style-span" style="border-collapse: collapse; font-family: sans-serif; -webkit-border-horizontal-spacing: 2px; -webkit-border-vertical-spacing: 2px; color: rgb(0, 0, 0); ">315-400 nm)</span>.</div>
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| - | <div>- These sunscreens use metal oxides (Zinc oxide) to absorb UV radiation, but the effects of absorbing these metals into your skin are not fully understood and are thought to lead to production of reactive oxygen species and could lead to melanomas rather than preventing them. </div>
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| - | <div>- Scytonemin is a pigment found in cyanobacteria which protects them from UV radiation, absorbing <span class="Apple-style-span" style="font-family: sans-serif; color: rgb(0, 0, 0); ">325-425 nm. Its synthesis requires three enzymes, SycA-C</span></div>
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| - | <div><span class="Apple-style-span" style="font-family: sans-serif; color: rgb(0, 0, 0); ">(http://www.int-res.com/articles/meps/158/m158p283.pdf)</span></div>
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| - | http://upload.wikimedia.org/wikipedia/commons/thumb/3/30/Scytonemin_biosynthesis.png/800px-Scytonemin_biosynthesis.png<br>
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| - | <div><span class="Apple-style-span" style="font-family: sans-serif; color: rgb(0, 0, 0); ">- M</span><span class="Apple-style-span" style="font-family: sans-serif; color: rgb(0, 0, 0); ">ycosporine-like amino acids (MAAs) are produced by organisms adapted to environments with high levels of sunlight (eg. cyanobacteria and algae), protecting them from UV radiation. There are 20 types and they also serve as anti-oxidants by stabilising free radicals (anti-ageing?). </span><span class="Apple-style-span" style="font-family: arial, verdana, helvetica, sans-serif; font-size: 12px; line-height: 18px; color: rgb(0, 0, 0); "> In a bioinformatics study the genes YP_324358 (predicted DHQ synthase) and YP_324357 (O-methyltransferase) were identified in <i style="box-sizing: border-box; ">A. variabilis</i> PCC 7937 cyanobacteria. (http://www.sciencedirect.com/science/article/pii/S0888754309002353)</span></div>
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| - | <b>Frank Machin<br>
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| - | </b>- First, I began to look into the possible production of the alkaloid isorhy, as was brought to my attention by Nina and Si<br>
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| - | - It is rumoured to be a potential treatment for Parkinson's and it would make a good project if this were to be produced by bacteria<br>
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| - | - On further research it turns out that the evidence for this drug as a treatment is weak and there is no information available about the gene or genes that encode it, so the idea was dropped<br>
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| - | - After being inspired by a student from the Royal College of Arts who presented us with her work on a project to create a living dress, I began to research the notion of a melanin tattoo, so that alpha-melanin stimulating hormone is applied to the skin and held in place until the skin darkens in the shape of the template. The alpha-MSH could be produced by bacteria.<br>
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| - | - the alpha-MSH gene is produced as one gene that also contains beta-MSH and gamma-MSH that are made available through post-transcriptional processing, so only the alpha-MSH region is required as it is the best characterised and has been expressed before<br>
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| - | - Once the alpha-MSH is expressed, it can be collected, soaked into silk (for example) that is cut into a pattern and will allow the hormone to diffuse into the skin, producing a (probably temporary) tattoo.<br>
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| - | - However, it seems that alpha-MSH is a rather powerful aphrodisiac and so a different hormone will have to be chosen, in addition, it seems that the hormone is unlikely to penetrate the skin as there are many different layers as well as proteases secreted by the skin
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| - | - alpha-MSH, or homologs are already used as tanning solutions
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| - | - Perhaps an alternative would be to use a mutant GFP that is able to glow brightly under normal light? This would have to be applied under the skin using a tattoo needle and could make for an interesting piece of body-art
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We had a briefing today chaired by James. Key action points of the day were to organize our CIDs, organize who was in charge of each team aspect and discuss the problems that we thought could be solved by Synthetic Biology. Once these problems had been discussed, we each chose one project that another team member came up with to research. We also were introduced to Professor Freemont and Professor Kitney who gave us an insightful talk about what awaits us. A trip to the Royal Society of Science exhibition ended up turning into a lunch in China town (the exhibition actually starts tomorrow) and we talked to Nicola Morgan who is interested in investigating the use of bacteria in making patterns on clothing.
(Text by Chris)
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