"
Team:Lyon-INSA-ENS/Realisation/Week18
From 2011.igem.org
(Created page with "{{Lyon-INSA-ENS/header}} {{Lyon-INSA-ENS/blocStyle}} {{INSA-Lyon/styletestaurelie}} {{Lyon-INSA-ENS/menuhorizontal}} {{Lyon-INSA-ENS/menuNotebookVertical|Week 18 = actif}} <htm...") |
|||
| (3 intermediate revisions not shown) | |||
| Line 38: | Line 38: | ||
<br/> <br/> | <br/> <br/> | ||
| + | <h6 style="text-align :left"> Monday </h6> <HR> | ||
| + | <br/> | ||
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | |||
| + | Extraction, EP Digestion and Gel electrophoresis of the NM522/p85 clones. They are all good. | ||
| + | |||
| + | |||
| + | <p/> | ||
| + | <br/><br/> | ||
| + | |||
| + | |||
| + | |||
| + | <h6 style="text-align :left"> Tuesday </h6> <HR> | ||
| + | <br/> | ||
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | |||
| + | Transformations : MC4100/pIG2+p150, MC4100/pIG6+p150. | ||
| + | Some problems with the transformations were noticed (only few clones), caused probably by the fact that the TSS wasn't fresh, so a new solution has been made and used. | ||
| + | |||
| + | <p/> | ||
| + | |||
| + | <br/><br/> | ||
| + | |||
| + | |||
| + | |||
| + | <h6 style="text-align :left"> Wednesday </h6> <HR> | ||
| + | <br/> | ||
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | Transformations results : no clones for MC4100/pIG2+p150, MC4100/pIG6+p150.<br/> | ||
| + | Transformations: MC4100/p85, PHL1414+piG3. | ||
| + | <p/> | ||
| + | <br/><br/> | ||
| + | |||
| + | |||
| + | <h6 style="text-align :left"> Friday </h6> <HR> | ||
| + | <br/> | ||
| + | <p style=" line-height : 1.5em"> | ||
| + | <FONT COLOR="red"> <b>Strain construction</b> </FONT> <br/><br/> | ||
| + | A 5ml liquid cultured for 4 clones of PHL1414/pIG3 and 4 clones of MC4100/p85 is started.<br/> | ||
| + | Extraction and digestion by E and P. | ||
| + | The four clones of MC4100/p85 are good. | ||
| + | <p/> | ||
| + | <br/><br/> | ||
| Line 43: | Line 89: | ||
<br/> <br/> | <br/> <br/> | ||
<br/> <br/> | <br/> <br/> | ||
| + | |||
| + | <p> | ||
| + | <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Week17"/><font color="grey"><b>Previous Week</b></font></a> | ||
| + | <a style = "float : right"; href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Week19"/><font color="grey"><b>Next Week</b></font></a> | ||
| + | <br/> | ||
| + | </p> | ||
| + | |||
<br/> <br/> | <br/> <br/> | ||
</div> | </div> | ||
Latest revision as of 18:20, 27 October 2011
Week 18
From Monday the 17th of October to Sunday the 23rd of October 2011
Monday
Strain construction
Extraction, EP Digestion and Gel electrophoresis of the NM522/p85 clones. They are all good.
Tuesday
Strain construction
Transformations : MC4100/pIG2+p150, MC4100/pIG6+p150.
Some problems with the transformations were noticed (only few clones), caused probably by the fact that the TSS wasn't fresh, so a new solution has been made and used.
Wednesday
Strain construction
Transformations results : no clones for MC4100/pIG2+p150, MC4100/pIG6+p150.
Transformations: MC4100/p85, PHL1414+piG3.
Friday
Strain construction
A 5ml liquid cultured for 4 clones of PHL1414/pIG3 and 4 clones of MC4100/p85 is started.
Extraction and digestion by E and P.
The four clones of MC4100/p85 are good.
