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Team:Paris Liliane Bettencourt/Notebook/2011/10/15/
From 2011.igem.org
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| + | ==Kevin== | ||
| + | ===Results of transformation=== | ||
| + | pVeg + SpoVG - YFP:tetR is OK (fluorescence at illuminatool).<br> | ||
| + | YFP:tetR - Double Term isn't good (after 12 PCR Colony, no one are ok). | ||
| + | |||
| + | === Creating pVeg + SpoVG - YFP:tetR - Double Term (the full construct) === | ||
| + | Digestion to transformation today. | ||
| + | |||
==Characterization of different strains in order to check if Subtilis or not== | ==Characterization of different strains in order to check if Subtilis or not== | ||
===Characterization of ComS=== | ===Characterization of ComS=== | ||
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{| border="1" class="wikitable" style="text-align: center;" | {| border="1" class="wikitable" style="text-align: center;" | ||
|- | |- | ||
| - | |[[File:ComS_exp_trans1_16_10.jpg|350px|thumb|center|ComS in | + | |[[File:ComS_exp_trans1_16_10.jpg|350px|thumb|center|ComS in exponential phase (trans image)]] |
| - | |[[File:comS_fluo_1_16_10. | + | |[[File:comS_fluo_1_16_10.jpg|350px|thumb|center|ComS in stationnary phase (gfp image)]] |
| - | |[[File:comS_fluo_2_16_10. | + | |[[File:comS_fluo_2_16_10.jpg|350px|thumb|center|ComS in stationnary phase (gfp image)]] |
| - | |[[File:comS_trans_1_16_10. | + | |[[File:comS_trans_1_16_10.jpg|350px|thumb|center|ComS in stationnary phase (trans image)]] |
| - | |[[File:comS_trans_1_16_10. | + | |
| - | |[[File:comS_trans_2_16_10. | + | |
| + | |||
| + | |} | ||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:comS_trans_1_16_10.jpg|350px|thumb|center|ComS in stationnary phase (trans image)]] | ||
| + | |[[File:comS_trans_2_16_10.jpg|350px|thumb|center|ComS in stationnary phase (trans image)]] | ||
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{| border="1" class="wikitable" style="text-align: center;" | {| border="1" class="wikitable" style="text-align: center;" | ||
|- | |- | ||
| - | |[[File:kinA_em_exp_trans_1_16_10. | + | |[[File:kinA_em_exp_trans_1_16_10.jpg|350px|thumb|center|KinA EM in exponential phase (trans image)]] |
| - | |[[File:kinA_em_exp_trans_2_16_10. | + | |[[File:kinA_em_exp_trans_2_16_10.jpg|350px|thumb|center|KinA EM in exponential phase (trans image)]] |
| - | |[[File:kinA_em_exp_trans_3_16_10. | + | |[[File:kinA_em_exp_trans_3_16_10.jpg|350px|thumb|center|KinA EM in exponential phase (trans image)]] |
| - | |[[File:kinA_em_trans_1_16_10. | + | |[[File:kinA_em_trans_1_16_10.jpg|350px|thumb|center|KinA EM in stationnary phase (trans image)]] |
| Line 30: | Line 44: | ||
{| border="1" class="wikitable" style="text-align: center;" | {| border="1" class="wikitable" style="text-align: center;" | ||
|- | |- | ||
| - | |[[File:kinA_rec_exp_trans_1_16_10. | + | |[[File:kinA_rec_exp_trans_1_16_10.jpg|350px|thumb|center|KinA REC in exponential phase (trans image)]] |
| - | |[[File:kinA_rec_exp_trans_2_16_10. | + | |[[File:kinA_rec_exp_trans_2_16_10.jpg|350px|thumb|center|KinA REC in exponential phase (trans image)]] |
| - | |[[File:kinA_rec_trans_1_16_10. | + | |[[File:kinA_rec_trans_1_16_10.jpg|350px|thumb|center|KinA REC in stationnary phase (trans image)]] |
| Line 38: | Line 52: | ||
===Characterization of RFP=== | ===Characterization of RFP=== | ||
| + | |||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:rfp_expo_fluo_1_16_10.jpg|350px|thumb|center|RFP in exponential phase (rfp image)]] | ||
| + | |[[File:rfp_expo_fluo_2_16_10.jpg|350px|thumb|center|RFP in exponential phase (rfp image)]] | ||
| + | |[[File:rfp_expo_fluo_3_16_10.jpg|350px|thumb|center|RFP in exponential phase (rfp image)]] | ||
| + | |||
| + | |||
| + | |} | ||
| + | |||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:rfp_expo_trans_1_16_10.jpg|350px|thumb|center|RFP in exponential phase (trans image)]] | ||
| + | |[[File:rfp_expo_trans_2_16_10.jpg|350px|thumb|center|RFP in exponential phase (trans image)]] | ||
| + | |[[File:rfp_expo_trans_3_16_10.jpg|350px|thumb|center|RFP in exponential phase (trans image)]] | ||
| + | |||
| + | |||
| + | |} | ||
| + | |||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:rfp_fluo_1_16_10.jpg|350px|thumb|center|RFP in stationnary phase (rfp image)]] | ||
| + | |[[File:rfp_fluo_2_16_10.jpg|350px|thumb|center|RFP in stationnary phase (rfp image)]] | ||
| + | |||
| + | |||
| + | |||
| + | |} | ||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:rfp_trans_1_16_10.jpg|350px|thumb|center|RFP in stationnary phase (trans image)]] | ||
| + | |[[File:rfp_trans_2_16_10.jpg|350px|thumb|center|RFP in stationnary phase (trans image)]] | ||
| + | |||
| + | |||
| + | |||
| + | |} | ||
| + | |||
| + | |||
| + | ===Characterization of T7 autoloop=== | ||
| + | |||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:t7_autoloop_exp_fluo_1_16_10.jpg|350px|thumb|center|KinA REC in exp phase (trans image)]] | ||
| + | |[[File:t7_autoloop_exp_fluo_2_16_10.jpg|350px|thumb|center|KinA REC in exp phase (trans image)]] | ||
| + | |[[File:t7_autoloop_exp_trans_1_16_10.jpg|350px|thumb|center|KinA REC in stationnary phase (trans image)]] | ||
| + | |||
| + | |||
| + | |} | ||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:t7_autoloop_exp_trans_2_16_10.jpg|350px|thumb|center|KinA REC in exp phase (trans image)]] | ||
| + | |[[File:t7_autoloop_fluo_1_16_10.jpg|350px|thumb|center|KinA REC in exp phase (trans image)]] | ||
| + | |[[File:t7_autoloop_fluo_2_16_10.jpg|350px|thumb|center|KinA REC in stationnary phase (trans image)]] | ||
| + | |||
| + | |||
| + | |} | ||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:t7_autoloop_trans_1_16_10.jpg|350px|thumb|center|KinA REC in exp phase (trans image)]] | ||
| + | |[[File:t7_autoloop_trans_2_16_10.jpg|350px|thumb|center|KinA REC in exp phase (trans image)]] | ||
| + | |||
| + | |||
| + | |} | ||
| + | |||
| + | ===Characterization of TetO=== | ||
| + | |||
| + | {| border="1" class="wikitable" style="text-align: center;" | ||
| + | |- | ||
| + | |[[File:teto_exp_trans_1_16_10.jpg|350px|thumb|center|KinA REC in exp phase (trans image)]] | ||
| + | |[[File:teto_exp_trans_2_16_10.jpg|350px|thumb|center|KinA REC in exp phase (trans image)]] | ||
| + | |[[File:teto_exp_trans_3_16_10.jpg|350px|thumb|center|KinA REC in stationnary phase (trans image)]] | ||
| + | |||
| + | |||
| + | |} | ||
Latest revision as of 20:45, 21 October 2011
Contents |
Kevin
Results of transformation
pVeg + SpoVG - YFP:tetR is OK (fluorescence at illuminatool).
YFP:tetR - Double Term isn't good (after 12 PCR Colony, no one are ok).
Creating pVeg + SpoVG - YFP:tetR - Double Term (the full construct)
Digestion to transformation today.
Characterization of different strains in order to check if Subtilis or not
Characterization of ComS
File:ComS exp trans1 16 10.jpg ComS in exponential phase (trans image) | File:ComS fluo 1 16 10.jpg ComS in stationnary phase (gfp image) | File:ComS fluo 2 16 10.jpg ComS in stationnary phase (gfp image) | File:ComS trans 1 16 10.jpg ComS in stationnary phase (trans image)
|
File:ComS trans 1 16 10.jpg ComS in stationnary phase (trans image) | File:ComS trans 2 16 10.jpg ComS in stationnary phase (trans image)
|
Characterization of KinA Emittor
File:KinA em exp trans 1 16 10.jpg KinA EM in exponential phase (trans image) | File:KinA em exp trans 2 16 10.jpg KinA EM in exponential phase (trans image) | File:KinA em exp trans 3 16 10.jpg KinA EM in exponential phase (trans image) | File:KinA em trans 1 16 10.jpg KinA EM in stationnary phase (trans image)
|
Characterization of KinA Receiver
File:KinA rec exp trans 1 16 10.jpg KinA REC in exponential phase (trans image) | File:KinA rec exp trans 2 16 10.jpg KinA REC in exponential phase (trans image) | File:KinA rec trans 1 16 10.jpg KinA REC in stationnary phase (trans image)
|
Characterization of RFP
File:Rfp expo fluo 1 16 10.jpg RFP in exponential phase (rfp image) | File:Rfp expo fluo 2 16 10.jpg RFP in exponential phase (rfp image) | File:Rfp expo fluo 3 16 10.jpg RFP in exponential phase (rfp image)
|
File:Rfp expo trans 1 16 10.jpg RFP in exponential phase (trans image) | File:Rfp expo trans 2 16 10.jpg RFP in exponential phase (trans image) | File:Rfp expo trans 3 16 10.jpg RFP in exponential phase (trans image)
|
File:Rfp fluo 1 16 10.jpg RFP in stationnary phase (rfp image) | File:Rfp fluo 2 16 10.jpg RFP in stationnary phase (rfp image)
|
File:Rfp trans 1 16 10.jpg RFP in stationnary phase (trans image) | File:Rfp trans 2 16 10.jpg RFP in stationnary phase (trans image)
|
Characterization of T7 autoloop
File:T7 autoloop exp fluo 1 16 10.jpg KinA REC in exp phase (trans image) | File:T7 autoloop exp fluo 2 16 10.jpg KinA REC in exp phase (trans image) | File:T7 autoloop exp trans 1 16 10.jpg KinA REC in stationnary phase (trans image)
|
File:T7 autoloop exp trans 2 16 10.jpg KinA REC in exp phase (trans image) | File:T7 autoloop fluo 1 16 10.jpg KinA REC in exp phase (trans image) | File:T7 autoloop fluo 2 16 10.jpg KinA REC in stationnary phase (trans image)
|
File:T7 autoloop trans 1 16 10.jpg KinA REC in exp phase (trans image) | File:T7 autoloop trans 2 16 10.jpg KinA REC in exp phase (trans image)
|
Characterization of TetO
File:Teto exp trans 1 16 10.jpg KinA REC in exp phase (trans image) | File:Teto exp trans 2 16 10.jpg KinA REC in exp phase (trans image) | File:Teto exp trans 3 16 10.jpg KinA REC in stationnary phase (trans image)
|
