Team:UC Davis

From 2011.igem.org

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Welcome to iGEM 2011 at UC Davis!<br><br>
Welcome to iGEM 2011 at UC Davis!<br><br>
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The four of us have been hard at work on this year's project. Things are coming along well -- we've fine tuned a powerful (and simple) mutant library generation process, created, screened and characterized a set of LacI promoter mutants, and created several TetR and λ cI promoter mutants for further characterization. We even mutated GFP!<br><br>
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The four of us have been hard at work on this year's project. We've fine tuned a powerful (and simple) part family generation process, and used it to expand the functionality of the LacI promoter, R0010. We also mutated GFP!<br><br>
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We also developed <a href="https://2011.igem.org/Team:UC_Davis/KO3D">KO3D</a>, a 3D javascript plotting library, for displaying characterization data for our mutants.<br><br>
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In addition, we developed <a href="https://2011.igem.org/Team:UC_Davis/KO3D">KO3D</a>, an interactive, cross-platform javascript plotting library for displaying scientific data on the web in 3D. <br><br>
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We had a great time at the regional competition in Indianapolis and look forward to seeing all of the other teams at the World Championship at MIT!
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We are excited for the regional competition in Indianapolis and look forward to seeing all of the other American teams there!
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<h1 id=Project>Our Project</h1>  
<h1 id=Project>Our Project</h1>  
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<p>This is the third time a team at UC Davis has competed in the iGEM Jamboree. This year, we are focusing on foundational advances for <a href="http://biobricks.org/" target="_blank">BioBrick</a> circuit synthesis. There is a need for more parts which have well-characterized variants which range in activity. Our project aims to streamline the process of mutant library generation from BioBrick parts, from promoters to protein-coding genes.<br><br>
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<p>This is the third time a team at UC Davis has competed in the iGEM Jamboree. This year, we are focusing on foundational advances for <a href="http://biobricks.org/" target="_blank">BioBrick</a> circuit synthesis. Part families, groups of parts with a unified function and differeing characteristics, are some of the most useful parts of the registry. Our project aims to streamline the process of expanding basic parts into part families, from promoters to protein-coding genes.
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<img src="http://farm7.static.flickr.com/6194/6084162068_09cbe08d11_b.jpg" width="250" align="left"></a>We set out to create useful mutant libraries of commonly used parts, starting with the repressible LacI promoter. You can read more about our project<a href="https://2011.igem.org/Team:UC_Davis/Project"> here.</a><br><br>
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<img src="http://farm7.static.flickr.com/6194/6084162068_09cbe08d11_b.jpg" width="250" align="left"></a>We set out to create useful mutant libraries of commonly used parts, starting with the repressible LacI, TetR and Lambda c1 promoters. You can read more about our project <a href="https://2011.igem.org/Team:UC_Davis/Project"> here.</a><br><br>
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We think that thorough characterization should be a priority for all parts submitted to the <a href="http://partsregistry.org" target="_blank">registry</a>. You can view information about our project<a href="https://2011.igem.org/Team:UC_Davis/Project"> here</a>. You can also review our characterization process and <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">characterization data for LacI</a> on our <a href="https://2011.igem.org/Team:UC_Davis/Data">data</a> page.<br><br>
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We think that thorough characterization should be a priority for all parts submitted to the <a href="http://partsregistry.org" target="_blank">registry</a>. You can view information about our project<a href="https://2011.igem.org/Team:UC_Davis/Project">here</a>. You can also review our characterization process and <a href="https://2011.igem.org/Team:UC_Davis/Data_LacI">characterization data for LacI</a> on our <a href="https://2011.igem.org/Team:UC_Davis/Data">data</a> page.<br><br>
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<h2>Want to Make Your Own Mutant Library?</h2>
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<h2>Make Your Own Part Family</h2>
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We developed a cheap and easy way to make part families which any team can do! Click <a href=""> here</a> to learn more about our process.  
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Our <a href="https://2011.igem.org/Team:UC_Davis/PartFamilies">part family expansion process</a> turns basic parts into part families. Check out how easy it is to improve your favorite BioBricks! You can also listen to Nick and Aaron discuss the process on our <a href="http://www.soundcloud.com/igem-at-uc-davis/part-families-rock">Soundcloud</a> page.
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<h2>Attributions</h2>
<h2>Attributions</h2>
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Credit for the various facets of our project, from <a href="https://2011.igem.org/Team:UC_Davis/Attributions#Wet">wetlab work</a> to <a href="https://2011.igem.org/Team:UC_Davis/Attributions#Wiki">graphic design</a> can be found on our <a href="https://2011.igem.org/Team:UC_Davis/Attributions">attributions</a> page, along with a list of the <a href="https://2011.igem.org/Team:UC_Davis/Attributions#opensource">awesome, free, open-source software</a> that we used to make this project happen.
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Credit for the various facets of our project, from wetlab work to graphic design can be found on our <a href="https://2011.igem.org/Team:UC_Davis/Attributions">attributions</a> page, along with a list of the awesome, free, open-source software that we used to make this project happen.
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<h2>Safety</h2>
 
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For more information on how we worked safely in our lab, please visit our <a href="https://2011.igem.org/Team:UC_Davis/Safety" style="color:#33FF33">Safety page.</a>
 
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Latest revision as of 23:54, 28 October 2011

Our Sponsors

Start a Family

Got a favorite BioBrick? Check our our process for expanding basic parts into part families.

Criteria

View our judging criteria for iGEM 2011 here.

Welcome

Welcome to iGEM 2011 at UC Davis!

The four of us have been hard at work on this year's project. We've fine tuned a powerful (and simple) part family generation process, and used it to expand the functionality of the LacI promoter, R0010. We also mutated GFP!


In addition, we developed KO3D, an interactive, cross-platform javascript plotting library for displaying scientific data on the web in 3D.

We had a great time at the regional competition in Indianapolis and look forward to seeing all of the other teams at the World Championship at MIT!

Our Project

This is the third time a team at UC Davis has competed in the iGEM Jamboree. This year, we are focusing on foundational advances for BioBrick circuit synthesis. Part families, groups of parts with a unified function and differeing characteristics, are some of the most useful parts of the registry. Our project aims to streamline the process of expanding basic parts into part families, from promoters to protein-coding genes. We set out to create useful mutant libraries of commonly used parts, starting with the repressible LacI promoter. You can read more about our project here.

We think that thorough characterization should be a priority for all parts submitted to the registry. You can view information about our project here. You can also review our characterization process and characterization data for LacI on our data page.

Make Your Own Part Family

Our part family expansion process turns basic parts into part families. Check out how easy it is to improve your favorite BioBricks! You can also listen to Nick and Aaron discuss the process on our Soundcloud page.

Team

The team page has information on the four undergrads that make up this year's team, their advisors, as well as contact information for getting in touch with us.


Safety

Our safety page discusses the potential impact of our project on environmental, public, and researcher safety. It also contains our answers to the iGEM 2011 safety questionnaire.

Notebook

The notebook page contains daily updates about our progress in lab, and also has a gallery where you can view pictures that we've taken in our lab.




Attributions

Credit for the various facets of our project, from wetlab work to graphic design can be found on our attributions page, along with a list of the awesome, free, open-source software that we used to make this project happen.