Team:ZJU-China/Safety.html

From 2011.igem.org

(Difference between revisions)
(Created page with "<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta...")
(Redirected page to Team:ZJU-China/Safety)
 
(One intermediate revision not shown)
Line 1: Line 1:
-
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
+
#REDIRECT [[Team:ZJU-China/Safety]]
-
<html xmlns="http://www.w3.org/1999/xhtml">
+
-
<head>
+
-
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8" />
+
-
<title>Human Practice</title>
+
-
<link href='http://fonts.googleapis.com/css?family=Molengo'
+
-
rel='stylesheet' type='text/css'>
+
-
<link href='http://fonts.googleapis.com/css?family=Hammersmith+One'
+
-
rel='stylesheet' type='text/css'>
+
-
<link href='http://fonts.googleapis.com/css?family=Alice'
+
-
rel='stylesheet' type='text/css'>
+
-
<link href='http://fonts.googleapis.com/css?family=Ovo' rel='stylesheet'
+
-
type='text/css'>
+
-
<link href='http://fonts.googleapis.com/css?family=Federo'
+
-
rel='stylesheet' type='text/css'>
+
-
 
+
-
<link href="http://zjuchina.tk/modeling.css" rel="stylesheet" type="text/css">
+
-
 
+
-
<link href="http://zjuchina.tk/global.css" rel="stylesheet" type="text/css">
+
-
 
+
-
<link href="http://zjuchina.tk/template2.css" rel="stylesheet" type="text/css"><!-- include the Tools -->
+
-
<script src="http://cdn.jquerytools.org/1.2.6/full/jquery.tools.min.js"></script>
+
-
 
+
-
<style type="text/css">
+
-
</style>
+
-
</head>
+
-
 
+
-
<body>
+
-
<div class="global">
+
-
<div id=top2></div>
+
-
<div class="header">
+
-
<div class="bgimg"></div>
+
-
<div class="bgpart1"></div>
+
-
<div class="bgpart2"></div>
+
-
 
+
-
<div class="banner">
+
-
<div class="logo"><img
+
-
src="https://static.igem.org/mediawiki/2011/c/c9/Logo.png" width="200"
+
-
height="200" /></div>
+
-
<div class="title"><img
+
-
src="http://ung.igem.org/wiki/images/b/b3/Title.png" width="603"
+
-
height="220" /></div>
+
-
<div class="igemlogo"><a target="_blank"
+
-
href="https://2011.igem.org"><img
+
-
src="https://static.igem.org/mediawiki/2011/e/e2/Igemlogo.png" width="180"
+
-
height="150" /></a></div>
+
-
</div>
+
-
<!--banner-->
+
-
<div class="nav">
+
-
<ul id="topnav">
+
-
<li><a id="P_Home" href="https://2011.igem.org/Team:ZJU-China/index.html">Home</a></li>
+
-
 
+
-
<li><a id="P_Project"
+
-
href="https://2011.igem.org/Team:ZJU-China/Project.html">Project</a> <!--Subnav Starts Here-->
+
-
 
+
-
 
+
-
<!--Subnav Ends Here--></li>
+
-
<li><a id="P_Team" href="https://2011.igem.org/Team:ZJU-China/Team.html">Team</a>
+
-
 
+
-
</li>
+
-
<li><a id="P_Notebook"
+
-
href="https://2011.igem.org/Team:ZJU-China/Notebook.html">Notebook</a></li>
+
-
<li><a id="P_HumanPractice"
+
-
href="https://2011.igem.org/Team:ZJU-China/Humanpractice.html">HumanPractice</a>
+
-
</li>
+
-
<li id="search">
+
-
<div id="searchbar">
+
-
 
+
-
<div>
+
-
<form action="http://www.google.com.hk/cse" id="cse-search-box"
+
-
target="_blank">
+
-
<div><input type="hidden" name="cx"
+
-
value="005438704919531788579:zbfi5n-q5du" /> <input type="hidden"
+
-
name="cof" value="FORID:10" /> <input type="hidden" name="ie"
+
-
value="UTF-8" /> <input type="text" name="q" size="15" /> <input
+
-
type="submit" name="sa" value="Search" /></div>
+
-
</form>
+
-
<script type="text/javascript"
+
-
src="http://www.google.com/cse/brand?form=cse-search-box&lang=en"></script></div>
+
-
</div>
+
-
</li>
+
-
</ul>
+
-
</div>
+
-
<!--navbar--> <script type="text/javascript">
+
-
  document.getElementById('P_Team').onclick=function(){
+
-
  document.getElementById('P_Te').style.display='block'
+
-
 
+
-
    document.getElementById('P_Pr').style.display='none'
+
-
document.getElementById('P_No').style.display='none'
+
-
document.getElementById('P_Hu').style.display='none'
+
-
<!-- document.getElementById('P_Pr').style.display='none' -->
+
-
  };
+
-
    document.getElementById('P_Project').onclick=function(){
+
-
  document.getElementById('P_Pr').style.display='block'
+
-
    document.getElementById('P_Te').style.display='none'
+
-
document.getElementById('P_No').style.display='none'
+
-
document.getElementById('P_Hu').style.display='none'
+
-
<!-- document.getElementById('P_Pr').style.display='none' -->
+
-
  };
+
-
    document.getElementById('P_Notebook').onclick=function(){
+
-
  document.getElementById('P_No').style.display='block'
+
-
    document.getElementById('P_Pr').style.display='none'
+
-
document.getElementById('P_Te').style.display='none'
+
-
document.getElementById('P_Hu').style.display='none'
+
-
<!-- document.getElementById('P_Pr').style.display='none' -->
+
-
  };
+
-
    document.getElementById('P_HumanPractice').onclick=function(){
+
-
  document.getElementById('P_Hu').style.display='block'
+
-
    document.getElementById('P_Pr').style.display='none'
+
-
document.getElementById('P_No').style.display='none'
+
-
document.getElementById('P_Te').style.display='none'
+
-
<!-- document.getElementById('P_Pr').style.display='none' -->
+
-
  };
+
-
 
+
-
</script></div>
+
-
<!--header-->
+
-
<div id="contentwrapper">
+
-
 
+
-
<div class="main">
+
-
<div class="bgpart3"></div>
+
-
 
+
-
<div class="leftulcontainer" id="purple">
+
-
<div id="leftul" class="leftul">
+
-
<div id="accordion">
+
-
 
+
-
<h4>Overview</h4>
+
-
 
+
-
<div class="pane" style="height: auto"><a
+
-
href="https://2011.igem.org/Team:ZJU-China/Humanpractice.html">Overview</a></div>
+
-
<h4 class="current">Safety</h4>
+
-
 
+
-
<div class="pane" style="display: block; height: auto"><a
+
-
href="https://2011.igem.org/Team:ZJU-China/Safety.html">Safety<br />
+
-
</a></div>
+
-
<h4>Originality</h4>
+
-
<div class="pane"><a href="https://2011.igem.org/Team:ZJU-China/App.html">iPhone
+
-
App<br />
+
-
</a> <a href="https://2011.igem.org/Team:ZJU-China/Novel.html">Novel<br />
+
-
</a></div>
+
-
<h4 style="font-size: 20px;">Funding Guide</h4>
+
-
 
+
-
<div class="pane"height:"auto"><a
+
-
href="https://2011.igem.org/Team:ZJU-China/Fundingreport.html">Funding Report</a>
+
-
<a href="https://2011.igem.org/Team:ZJU-China/Assessment.html">Assessment<br />
+
-
</a> <a href="https://2011.igem.org/Team:ZJU-China/Manual.html">Manual<br />
+
-
</a></div>
+
-
<h4><a href="https://2011.igem.org/Team:ZJU-China/SBC.html">SBC</a></h4>
+
-
 
+
-
<div class="pane" style="height: auto"><a
+
-
href="https://2011.igem.org/Team:ZJU-China/SBC.html">SBC<br />
+
-
</a></div>
+
-
 
+
-
 
+
-
 
+
-
<script>
+
-
$(function() {
+
-
+
-
$("#accordion").tabs("#accordion div.pane", {tabs: 'h4', effect: 'slide', initialIndex: null});
+
-
});
+
-
</script> <!-- Navigation scroll follow --> <script type="text/javascript">
+
-
        $(window).scroll(function () {
+
-
            var scrollPos = $(window).scrollTop();
+
-
            if (scrollPos > 330) {
+
-
                $(".leftul").addClass("stickToTop");
+
-
            } else {
+
-
                $(".leftul").removeClass("stickToTop");
+
-
            }
+
-
   
+
-
        });
+
-
    </script></div>
+
-
<!--accordion--></div>
+
-
</div>
+
-
<!--leftulcontainer-->
+
-
 
+
-
<div class="rightcontainer">
+
-
<div id="styleA" class="frame">
+
-
<div id="purple">
+
-
<h1>Safety</h1>
+
-
<!--importantcontainer-->
+
-
 
+
-
<div id="framecontent">
+
-
 
+
-
<div id="page1">
+
-
<div class="block" id="safety1">
+
-
<h1>Researcher safety</h1>
+
-
<hr />
+
-
<p>Our researchers mainly face regular bio-safety challenges in a
+
-
molecular microbiology lab, such as risks staining DNA agarose gel with
+
-
EB, extracting RNA from organisms with TRIZOL, and manipulating with
+
-
DEPC treated water, etc. Any researchers conducting such experiment<img
+
-
src="https://static.igem.org/mediawiki/2011/4/42/ZJUsafety2.jpg" width="300"
+
-
style="float: right;" /> are instructed about the right method both by
+
-
printed protocols and BIG BROTHER in the lab (XD). After the training,
+
-
we must pass an on-line test about the lab safety, standard experiment
+
-
procedure and lab waste treatment. During any experiments, necessary
+
-
individual protections such as gloves and white robes are always
+
-
required. Also, the public safety of the lab is guaranteed by the strict
+
-
registering protocols on the operation of any apparatus. In order to
+
-
make sure all the requirements above were followed well by the
+
-
researchers and to get prepared for any emergency, our lab (also is the
+
-
open bio-lab of the college) set up 24hr*7 camera system in the room.</p>
+
-
</div>
+
-
<div class="block" id="safety1">
+
-
<h1>Public safety</h1>
+
-
<hr />
+
-
<p>First we picked DH5α and JM109 as the two strains to manipulate
+
-
in our research. These are two non-pathogenic E. coli strain and do not
+
-
pose problems to the public health. The bio-parts we utilized are all
+
-
security ensured, coding for florescence protein, cellulose degradation
+
-
enzymes and metal ion binding proteins. Although bio-film formation is
+
-
closely related to pathogenicity, our experiment enhances bio-film
+
-
formation only by changing the external environment factors instead of
+
-
modifying the inherited nature of the bacteria. This is an important
+
-
measure to assure that the bacteria do not acquire previously
+
-
non-existing pathogenic capacity.</p>
+
-
</div>
+
-
<div class="block" id="safety1">
+
-
<h1>Environment safety</h1>
+
-
<hr />
+
-
<p>The experiments conducted in our lab are with intensive concern
+
-
of environmental impact. No media containing engineered germs go into
+
-
the sewer before sterilization. Besides, all the utensil contacted with
+
-
the engineered bacteria must first undergo sterilization then be washed.
+
-
During our research we have used antibiotic resistant plasmids,
+
-
including chloramphenicol, kanamycin, ampicillin. Though risks of the
+
-
practices exist, we believe that it's possible for us to use them safely
+
-
with the mature protocols and techniques. For the toxic and hazardous
+
-
chemicals, they are collected and treated properly. The acid and basic
+
-
chemicals are neutralized and pour into the swer.</p>
+
-
</div>
+
-
<div class="block" id="safety1">
+
-
<h1>Biobrick safety</h1>
+
-
<hr />
+
-
<p><img src="https://static.igem.org/mediawiki/2011/4/46/Zjusafety1.jpg"
+
-
width="220" style="float: left;">Our biobricks includes two new
+
-
promoters responded to oxygen concentration, hence the regulation of the
+
-
devices would not involve potentially bio-hazard chemicals. Also as
+
-
partially described above, our parts express proteins aiming at binding
+
-
of metal ions, degradation of cellulose, and the localization by
+
-
fluorescent proteins. All of these products are not bio-hazard, and the
+
-
expression is under fine control.</p>
+
-
</div>
+
-
<div class="block" id="safety1">
+
-
<h1>Biosafety provisions</h1>
+
-
<hr />
+
-
<p>Our experiment is designed and carried out following the
+
-
standards of national biosafety office, especially the <a
+
-
href="http://www.biosafety.gov.cn/gjzcfg/flfg/200401/t20040115_88044.htm"
+
-
target="_blank">《Safety Administration Regulation on Genetic
+
-
Engineering》</a> (Only the chinese version is available online),as well as <a
+
-
href="http://www.zjubiolab.zju.edu.cn/page/news.php?action=introlist&news_catalogId=33"
+
-
target="_blank">the laboratory regulations</a> of our university, the
+
-
college of life sciences, and the biology lab center.</p>
+
-
</div>
+
-
</div>
+
-
<script type="text/javascript">
+
-
document.getElementById('bb').onclick= function(){
+
-
document.getElementById('page2').style.display='none'
+
-
document.getElementById('page1').style.display='block'}
+
-
+
-
document.getElementById('bf').onclick= function(){
+
-
+
-
document.getElementById('page1').style.display='none'
+
-
document.getElementById('pgae2').style.display='block'}
+
-
</script>
+
-
<p style="clear: both"></p>
+
-
</div>
+
-
<!--framecontent-->
+
-
 
+
-
<div style="display: none" class="frame" id="rightnav">
+
-
<div class="bgcolors" id="round">
+
-
<p><a name="jumptop" id="bb">&nbsp; Biobrick Group&nbsp;</a>|<a
+
-
id="bf">&nbsp;Biofilm Group &nbsp;</a></p>
+
-
</div>
+
-
<!--bgcolors--></div>
+
-
<!--rightnav--></div>
+
-
<!--blue-->
+
-
<div class="links"><a href="#top2">Top</a></div>
+
-
</div>
+
-
<!--frame--></div>
+
-
<!--rightcontainer-->
+
-
<p style="clear: both"></p>
+
-
 
+
-
</div>
+
-
<!--main-->
+
-
<div class="footer" id="footer"><a href="https://2011.igem.org/">
+
-
iGem 2011 Home Page</a> <a href="http://ung.igem.org/Special:Upload/">
+
-
Upload Files</a> <a
+
-
href="https://2011.igem.org/wiki/index.php?title=Template:Zjucss_main&action=edit">Edit
+
-
CSS</a> <a href="http://ung.igem.org/Team_Wikis?year=2011"> Team Wikis</a> <a
+
-
href="mailto:zjuigem@gmail.com?"><strong>Contact Us</strong></a></div>
+
-
</div>
+
-
<!--contentwrapper--></div>
+
-
</body>
+
-
</html>
+

Latest revision as of 17:28, 19 October 2011

  1. REDIRECT Team:ZJU-China/Safety
Retrieved from "http://2011.igem.org/Team:ZJU-China/Safety.html"