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Team:IIT Madras/Notebook
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<div style="position:relative; top:50px;"><br/><br/><br/> | <div style="position:relative; top:50px;"><br/><br/><br/> | ||
| - | <H1>Notebook</H1> | + | <H1><u>Notebook</u></H1> |
<h2>Week 1</h2> | <h2>Week 1</h2> | ||
<li>Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin | <li>Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin | ||
| - | <li>Literature survey on expression of Proteorhodopsin in E.coli< | + | <li>Literature survey on expression of Proteorhodopsin in E.coli<br><br> |
<h2>Week 2</h2> | <h2>Week 2</h2> | ||
<li>Minimal media characterization of DH5-alpha | <li>Minimal media characterization of DH5-alpha | ||
<li>Modelling survival of PR transformed E.coli cells v/s wild type E.coli | <li>Modelling survival of PR transformed E.coli cells v/s wild type E.coli | ||
| - | <li>Literature survey on increase in recombinant protein expression in PR transformed cells< | + | <li>Literature survey on increase in recombinant protein expression in PR transformed cells<br><br> |
<h2>Week 3</h2> | <h2>Week 3</h2> | ||
<li>Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea. | <li>Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea. | ||
<li>Designing the incubator for experimental studies with Proteorhodopsin | <li>Designing the incubator for experimental studies with Proteorhodopsin | ||
| - | <li>Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide | + | <li>Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide<br><br> |
<h2>Week 4</h2> | <h2>Week 4</h2> | ||
<li>Standardizing the protocol for digestion of pKJ900 | <li>Standardizing the protocol for digestion of pKJ900 | ||
| - | <li>Designing RFP generator for promoter studies | + | <li>Designing RFP generator for promoter studies<br><br> |
<h2>Week5</h2> | <h2>Week5</h2> | ||
<li>Generating the PcstA_RFP Generator | <li>Generating the PcstA_RFP Generator | ||
| - | <li>Synthesizing the T7_RFP Generator | + | <li>Synthesizing the T7_RFP Generator<br><br> |
<h2>Week6</h2> | <h2>Week6</h2> | ||
<li>Characterizing PcstA_RFP Generator | <li>Characterizing PcstA_RFP Generator | ||
<li>Designing the promoters for Proteorhodopsin gene | <li>Designing the promoters for Proteorhodopsin gene | ||
| - | <li>Codon optimization of Proteorhodopsin gene | + | <li>Codon optimization of Proteorhodopsin gene<br><br> |
<h2>Week7</h2> | <h2>Week7</h2> | ||
<li>Designing the OmpR Promoter_RFP Generator | <li>Designing the OmpR Promoter_RFP Generator | ||
<li>Designing the PcstA_Luciferase Generator | <li>Designing the PcstA_Luciferase Generator | ||
| - | <li>Digestion of Promoters | + | <li>Digestion of Promoters<br><br> |
<h2>Week8</h2> | <h2>Week8</h2> | ||
<li>Transformation of Promoters | <li>Transformation of Promoters | ||
<li>Standardizing the protocols for transformation of proteorhodopsin | <li>Standardizing the protocols for transformation of proteorhodopsin | ||
| - | <li>Designing the Proteorhodopsin Generator | + | <li>Designing the Proteorhodopsin Generator<br><br> |
<h2>Week9</h2> | <h2>Week9</h2> | ||
<li>Preparation for Presentation at IITM Research Expo | <li>Preparation for Presentation at IITM Research Expo | ||
<li>Lecture preparation on synthetic biology | <li>Lecture preparation on synthetic biology | ||
| - | <li>Characterizing growth behaviour of Proteorhodopsin transformed cells on various minimal media under light/dark conditions. | + | <li>Characterizing growth behaviour of Proteorhodopsin transformed cells on various minimal media under light/dark conditions.<br><br> |
<h2>Week10</h2> | <h2>Week10</h2> | ||
<li>Expression studies of the recombinant protein in proteorhodopsin transformed E.coli | <li>Expression studies of the recombinant protein in proteorhodopsin transformed E.coli | ||
| - | <li>Presentation at IITM Research Expo | + | <li>Presentation at IITM Research Expo<br><br> |
<h2>Week11</h2> | <h2>Week11</h2> | ||
| - | <li>Wiki designing | + | <li>Wiki designing <br><br><br> |
Latest revision as of 21:56, 28 October 2011
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