Team:Kyoto/Material

From 2011.igem.org

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<table class="materials">
<table class="materials">
<tr>
<tr>
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<th>Name</th><th>Genotype and/or Relevant Features</th><th>Source or Reference
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<th>Name</th><th>Genotype and/or Relevant Features</th><!--<th>Source or Reference
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</th></tr>
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</th>--></tr>
<tr>
<tr>
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<td>D.melanogaster(fly)</td><td>Oregon-R-C</td><td>DGRC[http://kyotofly.kit.jp/cgi-bin/stocks/search_res_det.cgi?DB_NUM=1&DG_NUM=105669]
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<td>D.melanogaster(fly)</td><td>Oregon-RS</td><!--<td>DGRC[http://kyotofly.kit.jp/cgi-bin/stocks/search_res_det.cgi?DB_NUM=1&DG_NUM=105669]
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</td></tr>
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</td>--></tr>
</table>
</table>
<br/>
<br/>
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==Strains(E.Coli)==
==Strains(E.Coli)==
{| class="materials"
{| class="materials"
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|}
|}
<br/>
<br/>
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We got BW25113 from NIG, National Institute of Genetics.
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<br/>
 +
 +
==Strains(Streptomyces)==
 +
{| class="materials"
 +
!Name||Genotype and/or Relevant Features||Source or Reference
 +
|-
 +
| Streptomyces avermitilis
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| MA-4680,ATCC 31267,BCRC 12382
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|JCM Catalog[http://www.jcm.riken.jp/cgi-bin/jcm/jcm_keyword?AN=Streptomyces&BN=avermitilis&CN=&DN=]
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|-
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| Streptomyces albogriseolus
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| ATCC 23875, BCRC 12230
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|JCM Catalog[http://www.jcm.riken.jp/cgi-bin/jcm/jcm_keyword?AN=Streptomyces&BN=albogriseolus&CN=&DN=]
 +
|}
 +
<br/>
 +
We got these strains from JCM, Japan Collection of Microorganisms.
 +
<br/>
 +
==Primers for PCR amplification==
==Primers for PCR amplification==
{| class="materials"
{| class="materials"
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!Date||Name||Sequence
!Date||Name||Sequence
|-
|-
-
|07/21||S Forward (Same as SRRz Forward)||gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
+
|09/13||sigma54-f||TTGATCCGGTCGGTGTG
|-
|-
-
|07/21||S Reverse||CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
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|09/13||sigma54-r||AGTGCGGAAGTCGTGATTG
 +
|-
 +
|09/13||GAPDH-f||GGTGCGAAGAAAGTGGTTATG
 +
|-
 +
|09/13||GAPDH-r||TGGTGCAGGAAGCGTTG
 +
|-
 +
|09/13||GUS-f||GGCAACAAGCCGAAAGAAC
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|-
 +
|09/13||GUS-r||GGCAATACTCCACATCACCAC
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|-
 +
|09/13||TBP-f||CAATTCATCTCGACCATCACAC
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|-
 +
|09/13||TBP-r||AAATCCACCACCTCTTTGACC
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|-
 +
|09/13||PGK-f||TCAACAAAGGCGAGAAGAAAG
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|-
 +
|09/13||actin-f||ACGGTGTGGTTTACTCCTCTTC
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|-
 +
|09/13||PGK-r||GAACCGATTTCGTGCTTGATAC
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|-
 +
|09/23||GUS-f||ACAGCCAAAAGCCAGACAG
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|-
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|09/23||GUS-r||TGACGACCAAAGCCAGTAAAG
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|-
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|09/23||TBP-f||ACGGCTCATGGGATGTATG
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|-
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|09/23||TBP-r||ATCCCCAGTTTGATGGTTTG
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|-
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|09/23||glnL1-f||GAGATGGCTCCGATGGATAAC
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|-
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|09/23||glnL1-r||ACTAAATCACGGGCAGCAAC
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|-
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|09/23||glnL2-f||TGCTGCCCGTGATTTAGTG
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|-
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|09/23||glnL2-r||ATTCGAGTAGTGATGGGTCAGG
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|-
 +
|09/23||glnL3-f||GATACGCTGTTTTACCCGATG
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|-
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|09/23||glnL3-r||GATAGGCAGGTAAACCGAGAAC
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|-
 +
|09/23||glnG1-f||GGCGGTACATTATTCCTCGAC
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|-
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|09/23||glnG1-r||TCCGCACATCCACTTTCAC
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|-
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|09/23||glnG2-f||TCAGCGCCTATCAACAAGG
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|-
 +
|09/23||glnG2-r||TGCTGCTGTTCCTGGTAATG
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|-
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|09/23||glnG3-f||CAGATTAAACAGCGCCATCC
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|-
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|09/23||glnG3-r||CAAACGCCCCTTGTTGATAG
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|-
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|09/23||sigma54promoter-rpoN-f||actaGAGGTTCTGaacatgaagc
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|-
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|09/23||sigma54promoter-rpoN-r||attactctccagcgcctgc
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|-
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|09/23||rpoN-glnLG-f||tactagaGGAGACTGCTTTatggc
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|-
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|09/23||rpoN-glnLG-r||cgccaggttgtcatcgattaac
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|-
 +
|09/28||bolA-f||CGTTTTCTGAATCGTCATCG
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|-
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|09/28||bolA-f||CGTTTTCTGAATCGTCATCG
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|-
 +
|09/28||bolA-r||ACAGGGAGGAGAGGCAAAG
 +
|-
 +
|09/28||csiE1-f||GCTCCACCATCTGTCCTTTC
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|-
 +
|09/28||csiE1-r||GGCAATATCATCATCCACACC
 +
|-
 +
|09/28||csi2-r||ATTGTCTGTCCCGCTGATG
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|-
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|09/28||dps-f||GGGACCACTCAAGTTATCAACAG
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|-
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|09/28||dps-r||GGTCAGCCAGTTCTTTCAGG
|-
|-
|}
|}
----
----

Latest revision as of 00:29, 6 October 2011

Contents

Materials

Strain(Fruits Fly)

NameGenotype and/or Relevant Features
D.melanogaster(fly)Oregon-RS


Strains(E.Coli)

NameGenotype and/or Relevant FeaturesSource or Reference
JM109 endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15] Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
BW25113 ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514 E.coliwiki[http://ecoliwiki.net/colipedia/index.php/BW25113]


We got BW25113 from NIG, National Institute of Genetics.

Strains(Streptomyces)

NameGenotype and/or Relevant FeaturesSource or Reference
Streptomyces avermitilis MA-4680,ATCC 31267,BCRC 12382 JCM Catalog[http://www.jcm.riken.jp/cgi-bin/jcm/jcm_keyword?AN=Streptomyces&BN=avermitilis&CN=&DN=]
Streptomyces albogriseolus ATCC 23875, BCRC 12230 JCM Catalog[http://www.jcm.riken.jp/cgi-bin/jcm/jcm_keyword?AN=Streptomyces&BN=albogriseolus&CN=&DN=]


We got these strains from JCM, Japan Collection of Microorganisms.

Primers for PCR amplification

DateNameSequence
07/21S Forward (Same as SRRz Forward)gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
07/21S ReverseCTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
07/21SRRz ReverseCTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
07/28ΔTMD1 Forwardcgcggcagatataatggcggt
08/11ΔTMD1 Reversegtcatgtttttctggcatcttcatgtct
08/11glnL Forwardtctagaggagactgctttatggcaac
08/11glnL Reverseactagtaggaactatcgtcatcgactac
08/11glnG Forwardtctagaggtgacgtttatgcaacga
08/11glnG Reverseactagtacacacaagctgtgaatcactc
08/19, 08/24Sam7 Forwardggttcattcgtgaccttctcgacttc
08/19, 08/24Sam7 Reverseaggcgataatggcgcacatc
08/20SRRz Forward RetrygGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
08/20SRRz Reverse RetryCTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
08/23, 08/24Deletion of Upstream Forwardgagcaaatccccttattgggggtaagacatg
08/23, 08/24Deletion of Upstream Reversectctagaagcggccgcgaattcc
08/23c-glnL Forwardgctctagaggagactgctttatggcaac
08/23c-glnL Reverseggactagtaggaactatcgtcatcgactac
08/23c-glnG Forwardgctctagaggtgacgtttatgcaacga
08/23c-glnG Reverseggactagtacacacaagctgtgaatcactc
08/26gln-promoter1cggaattcgcggccgcttctagagattgca
08/26gln-promoter2rgaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
08/26gln-promoter3gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
08/26gln-promoter4rggactagtaaaagcgaaatctgtgccaacttttaaattgcccctaaaaggcgttatcatgcgcacc
08/28cc-glnL Forwardggaattcgcggccgcttctagaggagactgctttatggcaac
08/28cc-glnG Forwardggaattcgcggccgcttctagaggtgacgtttatgcaacga
08/28rpoN Forwardggaattcgcggccgcttctagaggttctgaacatgaagcaa
08/28rpoN Reverseggactagtatccttatcggttgggtca
08/31rpoN-Pstl-m2ggactagtagtagacgctgaccccgtag
09/06rpoN-Pstl-m1gcaagatgccaaatggttgatcaagagtc
09/12ChiA1 Forwardggaattcgcggccgcttctagagattggtctggaccagtttgta
09/12ChiA1 Reverseggactagtacactgccagttccacctacc
09/12serine proteae Forwardggaattcgcggccgcttctagagcctttgtggaggaacgaaac
09/12serine proteae Reverseggactagtagtagacgctgaccccgtag
09/13, 09/22Add J23105 ForwardCAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
09/13, 09/22Add J23105 ReverseAGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
10/10Deletion of GFP Forward (Primer1)ccaggcatcaaataaaacgaaaggctc
10/10Deletion of GFP-DT Forward (Primer2)tactagtagcggccgctgc
10/10Deletion of GFP, GFP-DT Reverse (Primer3)ctctagtattattgatttctaccatcttctactcc
- VF2 (<partinfo>G00100</partinfo>)tgccacctgacgtctaagaa
- VF (<partinfo>G00101</partinfo>)attaccgcctttgagtgagc


Primers for Real time PCR

DateNameSequence
09/13sigma54-fTTGATCCGGTCGGTGTG
09/13sigma54-rAGTGCGGAAGTCGTGATTG
09/13GAPDH-fGGTGCGAAGAAAGTGGTTATG
09/13GAPDH-rTGGTGCAGGAAGCGTTG
09/13GUS-fGGCAACAAGCCGAAAGAAC
09/13GUS-rGGCAATACTCCACATCACCAC
09/13TBP-fCAATTCATCTCGACCATCACAC
09/13TBP-rAAATCCACCACCTCTTTGACC
09/13PGK-fTCAACAAAGGCGAGAAGAAAG
09/13actin-fACGGTGTGGTTTACTCCTCTTC
09/13PGK-rGAACCGATTTCGTGCTTGATAC
09/23GUS-fACAGCCAAAAGCCAGACAG
09/23GUS-rTGACGACCAAAGCCAGTAAAG
09/23TBP-fACGGCTCATGGGATGTATG
09/23TBP-rATCCCCAGTTTGATGGTTTG
09/23glnL1-fGAGATGGCTCCGATGGATAAC
09/23glnL1-rACTAAATCACGGGCAGCAAC
09/23glnL2-fTGCTGCCCGTGATTTAGTG
09/23glnL2-rATTCGAGTAGTGATGGGTCAGG
09/23glnL3-fGATACGCTGTTTTACCCGATG
09/23glnL3-rGATAGGCAGGTAAACCGAGAAC
09/23glnG1-fGGCGGTACATTATTCCTCGAC
09/23glnG1-rTCCGCACATCCACTTTCAC
09/23glnG2-fTCAGCGCCTATCAACAAGG
09/23glnG2-rTGCTGCTGTTCCTGGTAATG
09/23glnG3-fCAGATTAAACAGCGCCATCC
09/23glnG3-rCAAACGCCCCTTGTTGATAG
09/23sigma54promoter-rpoN-factaGAGGTTCTGaacatgaagc
09/23sigma54promoter-rpoN-rattactctccagcgcctgc
09/23rpoN-glnLG-ftactagaGGAGACTGCTTTatggc
09/23rpoN-glnLG-rcgccaggttgtcatcgattaac
09/28bolA-fCGTTTTCTGAATCGTCATCG
09/28bolA-fCGTTTTCTGAATCGTCATCG
09/28bolA-rACAGGGAGGAGAGGCAAAG
09/28csiE1-fGCTCCACCATCTGTCCTTTC
09/28csiE1-rGGCAATATCATCATCCACACC
09/28csi2-rATTGTCTGTCCCGCTGATG
09/28dps-fGGGACCACTCAAGTTATCAACAG
09/28dps-rGGTCAGCCAGTTCTTTCAGG
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