Team:HKUST-Hong Kong/notebook.html
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<br>Notebook</font></p> | <br>Notebook</font></p> | ||
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<p><strong>Week 1 (4th-10th June)</strong><br> | <p><strong>Week 1 (4th-10th June)</strong><br> | ||
- | Strain construction | + | Strain construction</p></font> |
<ul> | <ul> | ||
<li>Genomic DNA of <em>E. coli DH10b</em> extracted</li> | <li>Genomic DNA of <em>E. coli DH10b</em> extracted</li> | ||
- | <li>Waiting for materials to arrive</li> | + | <li>Waiting for materials (bacterial strains, primers) to arrive</li> |
- | </ul> | + | </ul><br> |
- | <p><strong>Week </strong><strong>2</strong><strong> ( | + | <p><strong>Week </strong><strong>2</strong><strong> (13th-17th June)</strong><strong> </strong><br> |
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
- | <li>Genomic DNA of <em>E. coli </em> DH10b extracted Genomic DNA of BL21 extracted</li> | + | <li>Genomic DNA of <em>E. coli </em> DH10b extracted. Genomic DNA of BL21 extracted</li> |
- | <li> | + | <li>Cloned out split superfolder GFP construct </li> |
- | <li>Finished design of PCR primers</li> | + | <li>Finished design of PCR primers for nadE gene and λ RED</li> |
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
<li>Wild type MIC test optimization (kanamycin gradient 0-25 µg/ml, serial dilutions)</li> | <li>Wild type MIC test optimization (kanamycin gradient 0-25 µg/ml, serial dilutions)</li> | ||
- | <li>Constructed standard curve for OD600 verses RR1 CFU concentration </li> | + | <li>Constructed standard curve for OD600 verses RR1 (wild type) CFU concentration </li> |
</ul> | </ul> | ||
- | + | <br> | |
<p><strong>Week </strong><strong>3</strong><strong> (20th-24th June)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>3</strong><strong> (20th-24th June)</strong><strong> </strong><br> | ||
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
<li><i>nadE</i>: PCR out <i>nadE</i> gene from the genome of BL21 </li> | <li><i>nadE</i>: PCR out <i>nadE</i> gene from the genome of BL21 </li> | ||
- | <li>Split superfolder GFP system: The test of the intact superfolder GFP | + | <li>Split superfolder GFP system: The test of the intact (sfGFP 1-10 still ligated together with sfGFP11) superfolder GFP was successful. Confirmation tests and further experiments would be conducted soon.</li> |
- | <li>2010 Slovenia’s method - CFP/YFP: BioBricks | + | <li>2010 Slovenia’s method - CFP/YFP: BioBricks would be transformed into <em>E. coli</em> DH10b. Tests would be conducted soon.</li> |
- | <li>λ RED and oriR101&repA101-ts: pKD46 has arrived and | + | <li>λ RED and oriR101&repA101-ts: pKD46 has arrived and successfully extracted. RFP reporter system was ready. Primers of RepA101ts-OriR101 are ready. </li> |
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
- | <li>Performed 2nd and 3rd MIC | + | <li>Performed 2nd and 3rd MIC tests for wild type (kanamycin gradient 5-20 µg/ml, 2µg/ml intervals) </li> |
- | <li> | + | <li>Minipreps of BBa_I763007 and BBa_E1010 were successful </li> |
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>4</strong><strong> (2</strong><strong>7</strong><strong>th</strong><strong> June–1st</strong><strong> Ju</strong><strong>ly</strong><strong>)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>4</strong><strong> (2</strong><strong>7</strong><strong>th</strong><strong> June–1st</strong><strong> Ju</strong><strong>ly</strong><strong>)</strong><strong> </strong><br> | ||
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
- | <li>λ RED : protocol design finished | + | <li>λ RED : protocol design finished; pKD46 arrived and digestion tests indicated that the plasmid was correct; PCR RFP with homologous sequence was successful</li> |
<li>2010 Slovenia’s method - CFP/YFP: protocol design finished, successfully finished combined CFP and YFP</li> | <li>2010 Slovenia’s method - CFP/YFP: protocol design finished, successfully finished combined CFP and YFP</li> | ||
- | <li>Split superfolder GFP system: protocol design finished | + | <li>Split superfolder GFP system: protocol design finished; primer arrived</li> |
- | <li>Pir gene and ori-γ: protocol under construction | + | <li>Pir gene and ori-γ: protocol under construction; primer for ori-γ arrived; BW25141 gDNA extraction successful</li> |
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
- | <li>Ligation of pSB2K3 (from BBa_E1010) with RFP | + | <li>Ligation of pSB2K3 (from BBa_E1010) with RFP reporter device (BBa_I763007) </li> |
<li>Transformation of the RFP/KanR plasmid to <em>E. coli </em>DH10b</li> | <li>Transformation of the RFP/KanR plasmid to <em>E. coli </em>DH10b</li> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>5</strong><strong> (</strong><strong>8th-12th </strong><strong>Ju</strong><strong>ly</strong><strong>)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>5</strong><strong> (</strong><strong>8th-12th </strong><strong>Ju</strong><strong>ly</strong><strong>)</strong><strong> </strong><br> | ||
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
- | <li>ori-γ: Primers arrived, PCR was successful. Result: one of four samples | + | <li>ori-γ: Primers arrived, PCR was successful. Result: one of four samples was positive, but of low concentration</li> |
- | <li>pir gene: gDNA of BW25141 extracted</li> | + | <li>pir gene: gDNA of BW25141 successfully extracted</li> |
<li>Split superfolder GFP system: GFP1-10 PCRed, GFP11 PCRed</li> | <li>Split superfolder GFP system: GFP1-10 PCRed, GFP11 PCRed</li> | ||
<li>2010 Slovenia’s method - CFP/YFP: Verified combined fluorescence protein</li> | <li>2010 Slovenia’s method - CFP/YFP: Verified combined fluorescence protein</li> | ||
- | <li>oriR101&repA101-ts: PCR was successful</li> | + | <li>oriR101 & repA101-ts: Clone- out by PCR was successful</li> |
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
- | <li>Successful construction of a RFP-labeled kanamycin-resistant strain | + | <li>Successful construction of a RFP-labeled kanamycin-resistant strain</li> |
- | <li> | + | <li>Information from literature search on mechanisms to raise the MIC for the proposed T4MO mutant slightly help it survive in kanamycin long enough to fulfill its function</li> |
<li>MIC testing for RR-1 </li> | <li>MIC testing for RR-1 </li> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>6</strong><strong> (</strong><strong>15th-19th </strong><strong>Ju</strong><strong>ly</strong><strong>)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>6</strong><strong> (</strong><strong>15th-19th </strong><strong>Ju</strong><strong>ly</strong><strong>)</strong><strong> </strong><br> | ||
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
- | <li>λ RED: RFP with homologous sequence | + | <li>λ RED: PCR of RFP with homologous sequence successful</li> |
- | <li>2010 Slovenia’s method - CFP/YFP: digestion and ligation of CFP, YFP with pBluescript promoter finished</li> | + | <li>2010 Slovenia’s method - CFP/YFP: digestion and ligation of CFP, YFP with pBluescript KS+ promoter finished</li> |
- | <li>Split superfolder GFP system : PCR of spilt superfolder GFP successful</li> | + | <li>Split superfolder GFP system: PCR of spilt superfolder GFP successful</li> |
- | <li>2010 Slovenia’s method - CFP/YFP :Ligation with promoter is successful, but | + | <li>2010 Slovenia’s method - CFP/YFP :Ligation with promoter is successful, but the green fluorescence could not be observed. Considering to redo construction</li> |
- | <li>pToolkit construction: PCR of ori-γ successful | + | <li>pToolkit construction: PCR of ori-γ successful; ligation with pKD46 backbone was done, confirmation still awaiting the results of colony PCR to check the existence of ori-γ; the sequencing PCR of the pir gene was done, now waiting to check the results</li> |
- | <li><i>nadE</i> gene: | + | <li><i>nadE</i> gene: ligation <i>nadE</i> gene with double terminator not successful. Would repeat experiments next week</li> |
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
<li>MIC test for wild type RR1 (kanamycin gradient 5-13 µg/ml, 1µg/ml intervals) </li> | <li>MIC test for wild type RR1 (kanamycin gradient 5-13 µg/ml, 1µg/ml intervals) </li> | ||
Line 153: | Line 141: | ||
<ul> | <ul> | ||
<li>Bcr(~1.2kbp): overexpression increases kanamycin MIC ~2-4fold </li> | <li>Bcr(~1.2kbp): overexpression increases kanamycin MIC ~2-4fold </li> | ||
- | <li>NorM (~1.3kbp): | + | <li>NorM (~1.3kbp): overexpression reduces radical oxidative species (e.g. H<sub>2</sub>O<sub>2</sub>) inside the cell</li> |
</ul> | </ul> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>7 </strong><strong>(</strong><strong>22nd-26th </strong><strong>Ju</strong><strong>ly</strong><strong>)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>7 </strong><strong>(</strong><strong>22nd-26th </strong><strong>Ju</strong><strong>ly</strong><strong>)</strong><strong> </strong><br> | ||
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
- | <li>pir gene: Sequencing product did not meet sequencing requirement – sequencing rejected</li> | + | <li>pir gene: Sequencing product did not meet sequencing requirement (a lot of 'N's) – sequencing rejected. To do: practice how to perform sequencing clean-up properly</li> |
- | <li>Split superfolder GFP system: | + | <li>Split superfolder GFP system: Construct to be ligated had very low recovery from gel purification; another trial would be done asap </li> |
- | <li>2010 Slovenia’s method | + | <li>2010 Slovenia’s method – CFP/YFP: combination of n-terminal and c-terminal CDS into same plasmid, driven by plac promoter of pBluescriptKS+ completed. Result: very weak fluorescence observed</li> |
- | <li><em>nadE</em> gene: successful | + | <li><em>nadE</em> gene: successful ligation of nadE gene with terminator; correctness of construct confirmed by restriction digestion tests. Component was putatively finished as biobrick</li> |
<li>oriR101&repA101-ts: basic protocol for site-directed-mutagenesis + fusion PCR tested to be successful. Repeating fusion PCR</li> | <li>oriR101&repA101-ts: basic protocol for site-directed-mutagenesis + fusion PCR tested to be successful. Repeating fusion PCR</li> | ||
<li>λ RED: Previous experiment of gene swapping failed. Trouble-shooting in progress</li> | <li>λ RED: Previous experiment of gene swapping failed. Trouble-shooting in progress</li> | ||
- | <li>pToolkit construction: results from colony PCR of ori-γ from transformed bacteria: successful completion of pToolkit</li> | + | <li>pToolkit construction: results from colony PCR of ori-γ from transformed bacteria: successful completion of pToolkit. Further confirmation by restriction digestion to be done</li> |
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
- | <li>Mixed culture MIC tests for RFP/KanR and RR1( | + | <li>Mixed culture MIC tests for RFP/KanR and RR1(99:1)</li> |
<li>Multidrug Efflux Pump – Settled on Bcr as the candidate gene </li> | <li>Multidrug Efflux Pump – Settled on Bcr as the candidate gene </li> | ||
<ul> | <ul> | ||
Line 177: | Line 165: | ||
<li>Unknown promoter </li> | <li>Unknown promoter </li> | ||
</ul> | </ul> | ||
- | <li><em>E. coli</em> DH10a containing pUC18Not/T4MO arrived | + | <li><em>E. coli</em> DH10a containing pUC18Not/T4MO arrived</li> |
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>8 </strong><strong>(</strong><strong>1st-5th Aug</strong><strong>)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>8 </strong><strong>(</strong><strong>1st-5th Aug</strong><strong>)</strong><strong> </strong><br> | ||
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
<li>pir gene: Sequencing result has just come out</li> | <li>pir gene: Sequencing result has just come out</li> | ||
<li>Split superfolder GFP system: Finished ligation of lacI promotor and GFP 11 and verifying. GFP 1-10 PCRing</li> | <li>Split superfolder GFP system: Finished ligation of lacI promotor and GFP 11 and verifying. GFP 1-10 PCRing</li> | ||
<li>2010 Slovenia’s method - CFP/YFP: Finished construction but not verified</li> | <li>2010 Slovenia’s method - CFP/YFP: Finished construction but not verified</li> | ||
- | <li><i>nadE</i> gene: finished</li> | + | <li><i>nadE</i> gene: construction finished, but construct was still harbored in pSB1AK3. Consider relocation to pSB1C3 asap.</li> |
<li>oriR101&repA101-ts: Been ligated to a backbone, verifying</li> | <li>oriR101&repA101-ts: Been ligated to a backbone, verifying</li> | ||
- | <li>λ RED: Waiting for the primers to construct the linear sequence</li> | + | <li>λ RED: Waiting for the primers to construct the linear dsDNA sequence (for swapping)</li> |
+ | <li>pCarrier: Design of Multiple Cloning Site sequence had been completed, waiting for oligos to arrive. | ||
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
- | <li>Completed the standard curve for | + | <li>Completed the standard curve for OD600 versus RFP/KanR CFU concentration</li> |
<li>Mixed culture MIC tests for RFP/KanR and RR1(1:99)</li> | <li>Mixed culture MIC tests for RFP/KanR and RR1(1:99)</li> | ||
<li>Successfully extracted T4MO from pUC18Not/T4MO, discovering the inclusion of a native constitutive promoter, and ligated to pBlueScript KS+ to create a SpeI site for biobrick assembly. </li> | <li>Successfully extracted T4MO from pUC18Not/T4MO, discovering the inclusion of a native constitutive promoter, and ligated to pBlueScript KS+ to create a SpeI site for biobrick assembly. </li> | ||
<li>PCR amplified <em>bcr </em>gene from gDNA of <em>E. coli </em>stock </li> | <li>PCR amplified <em>bcr </em>gene from gDNA of <em>E. coli </em>stock </li> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>9 (</strong><strong>8th-12th Aug)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>9 (</strong><strong>8th-12th Aug)</strong><strong> </strong><br> | ||
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
<li>pir gene: Exact location of pir gene in BW25141 is mapped out</li> | <li>pir gene: Exact location of pir gene in BW25141 is mapped out</li> | ||
- | <li>Split superfolder GFP system: Primers | + | <li>Split superfolder GFP system: Primers did not work: a lot of non- specific bindings, expected band size was not clearly present. New primers had been designed; waiting for new primers to come next week</li> |
- | <li>2010 Slovenia’s method - CFP/YFP: CFP ligated with pET. YFP | + | <li>2010 Slovenia’s method - CFP/YFP: CFP ligated with pET. YFP still on progress</li> |
<li>oriR101&repA101-ts: Verifying oriR101&repA101-ts </li> | <li>oriR101&repA101-ts: Verifying oriR101&repA101-ts </li> | ||
<li>λ RED: PCR with the new primers is successful. Modified protocol using KAN-resistance gene to swap out uidA gene</li> | <li>λ RED: PCR with the new primers is successful. Modified protocol using KAN-resistance gene to swap out uidA gene</li> | ||
- | <li>pCarrier: MCS | + | <li>pCarrier: MCS had been hybridized. pSB1K3 is under digestion</li> |
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
<li>Digestion of T4MO/pBS KS+ failed</li> | <li>Digestion of T4MO/pBS KS+ failed</li> | ||
<li>Successfully ligated <i>bcr</i> gene with RBS (later confirmed to be false positive) </li> | <li>Successfully ligated <i>bcr</i> gene with RBS (later confirmed to be false positive) </li> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week 10 (15th-19th Aug) </strong><br> | <p><strong>Week 10 (15th-19th Aug) </strong><br> | ||
Strain construction<strong></strong></p> | Strain construction<strong></strong></p> | ||
Line 218: | Line 207: | ||
<li>λ RED: The swapping seemed to be successful</li> | <li>λ RED: The swapping seemed to be successful</li> | ||
<li>oriR101&repA101-ts: Waiting for new primers</li> | <li>oriR101&repA101-ts: Waiting for new primers</li> | ||
- | <li>pCarrier: MCS and | + | <li>pCarrier: MCS and pSB1AK3 with nadE ligated, but not confirmed </li> |
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
<li>Indole MIC test for wild type (1mM with kanamycin gradient): </li> | <li>Indole MIC test for wild type (1mM with kanamycin gradient): </li> | ||
<li>Successfully ligated T4MO into pBS KS+</li> | <li>Successfully ligated T4MO into pBS KS+</li> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>11 (</strong><strong>22nd-26th Aug)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>11 (</strong><strong>22nd-26th Aug)</strong><strong> </strong><br> | ||
- | Strain construction | + | Strain construction</p> |
<ul> | <ul> | ||
<li>pir gene: ligation of pir gene and pBluescriptK+, repeating dephosphorylation to prevent self-ligation of pBluescriptKS+ backbone<strong></strong></li> | <li>pir gene: ligation of pir gene and pBluescriptK+, repeating dephosphorylation to prevent self-ligation of pBluescriptKS+ backbone<strong></strong></li> | ||
<li>Split superfolder GFP system: Re-digestion and dephosphorylate R0010 in pSB1AK3, to reduce background self-ligation during transformation<strong> </strong></li> | <li>Split superfolder GFP system: Re-digestion and dephosphorylate R0010 in pSB1AK3, to reduce background self-ligation during transformation<strong> </strong></li> | ||
- | <li>2010 Slovenia’s method - CFP/YFP: digestion and ligation of pET_YFP; checking construct of pET_YFP; checking fluorescence | + | <li>2010 Slovenia’s method - CFP/YFP: digestion and ligation of pET_YFP; checking construct of pET_YFP; checking fluorescence<strong> </strong></li> |
<li><em>nadE</em> gene: Complete.<strong> </strong></li> | <li><em>nadE</em> gene: Complete.<strong> </strong></li> | ||
<li>oriR101&repA101-ts: ligation of oriR101, repA101 and the backbone pSA1K3 in process; transformation result available tomorrow; colony PCR of λ red done, failed.<strong> </strong></li> | <li>oriR101&repA101-ts: ligation of oriR101, repA101 and the backbone pSA1K3 in process; transformation result available tomorrow; colony PCR of λ red done, failed.<strong> </strong></li> | ||
Line 236: | Line 225: | ||
<li>pCarrier: Ligation of MCS to <i>nadE</i> in pSB1AK3 is complete; Digestion check showed negative result; Hybridization of MCS in progress<strong> </strong></li> | <li>pCarrier: Ligation of MCS to <i>nadE</i> in pSB1AK3 is complete; Digestion check showed negative result; Hybridization of MCS in progress<strong> </strong></li> | ||
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test<strong></strong></p> |
<ul> | <ul> | ||
<li>Indole MIC test (500µM with kanamycin gradient)</li> | <li>Indole MIC test (500µM with kanamycin gradient)</li> | ||
<li>Ligation of the RBS+Bcr with pLac promoter failed </li> | <li>Ligation of the RBS+Bcr with pLac promoter failed </li> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>12 (</strong><strong>29th Aug-1st Sep)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>12 (</strong><strong>29th Aug-1st Sep)</strong><strong> </strong><br> | ||
Strain Construction</p> | Strain Construction</p> | ||
Line 253: | Line 242: | ||
<li>pCarrier: re-annealing of ssDNA of MCS; Re-planning of insertion position of MCS </li> | <li>pCarrier: re-annealing of ssDNA of MCS; Re-planning of insertion position of MCS </li> | ||
</ul> | </ul> | ||
- | <p>Culture | + | <p>Culture Test</p> |
<ul> | <ul> | ||
<li>Mixed culture MIC tests for RFP/KanR and RR1 (1:99) </li> | <li>Mixed culture MIC tests for RFP/KanR and RR1 (1:99) </li> | ||
<li>Indole MIC test (300µM with kanamycin gradient)</li> | <li>Indole MIC test (300µM with kanamycin gradient)</li> | ||
<li>Successfully ligated T4MO with GFP</li> | <li>Successfully ligated T4MO with GFP</li> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week </strong><strong>13 (</strong><strong>5th-9th Sep)</strong><strong> </strong><br> | <p><strong>Week </strong><strong>13 (</strong><strong>5th-9th Sep)</strong><strong> </strong><br> | ||
Strain construction</p> | Strain construction</p> | ||
Line 278: | Line 267: | ||
<li>Successfully ligated T4MO/GFP into kanamycin resistant backbone. </li> | <li>Successfully ligated T4MO/GFP into kanamycin resistant backbone. </li> | ||
</ul> | </ul> | ||
- | + | <br> | |
- | <p><strong>Week </strong><strong>14 (</strong><strong> | + | <p><strong>Week </strong><strong>14 (</strong><strong>13th-17th Sep)</strong> <br> |
- | Strain Construction | + | Strain Construction</p> |
<ul> | <ul> | ||
<li>λ RED:basically successful; new S2 primers arrived, first trial failed (negative control of pKD46+<i>E. coli</i> DH10B still have some bands); consider directly PCR out from pKD46 </li> | <li>λ RED:basically successful; new S2 primers arrived, first trial failed (negative control of pKD46+<i>E. coli</i> DH10B still have some bands); consider directly PCR out from pKD46 </li> | ||
Line 295: | Line 284: | ||
<li>Indole MIC test (1mM and 2mM with kanamycin gradient) [2mM experiment failed] </li> | <li>Indole MIC test (1mM and 2mM with kanamycin gradient) [2mM experiment failed] </li> | ||
<li>Started to construct BioBrick of bcr gene for submission</li> | <li>Started to construct BioBrick of bcr gene for submission</li> | ||
- | </ul> | + | </ul><br> |
<p><strong>Week 15 </strong> <strong>(</strong><strong>20th-24th</strong><strong> Sep)</strong> <br> | <p><strong>Week 15 </strong> <strong>(</strong><strong>20th-24th</strong><strong> Sep)</strong> <br> | ||
- | Strain Construction | + | Strain Construction</p> |
<ul> | <ul> | ||
- | <li>oriR101&repA101-ts: the progress is not | + | <li>oriR101&repA101-ts: the progress is not ideal, cannot finishthe characterizationthis week </li> |
<li>pir gene: sequence result: some key parts are missing. the target part developed an unexpected illegal cut (point mutation or star activity); insert the pir to pBS again (using different enzymes), sequenced again. </li> | <li>pir gene: sequence result: some key parts are missing. the target part developed an unexpected illegal cut (point mutation or star activity); insert the pir to pBS again (using different enzymes), sequenced again. </li> | ||
<li>Split superfolder GFP system: the construction of split GFP+backbone finished; characterization in progress </li> | <li>Split superfolder GFP system: the construction of split GFP+backbone finished; characterization in progress </li> | ||
- | </ul> | + | </ul><br> |
- | <p><strong>Week 16 ( 27th-30th Sep)</strong><br> | + | <p><strong>Week 16 (27th-30th Sep)</strong><br> |
Strain Construction</p> | Strain Construction</p> | ||
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+ | </TH> | ||
+ | </TD> | ||
+ | </TR> | ||
+ | </table> | ||
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+ | <table border="0" cellspacing="0" cellpadding="10" width=963> | ||
+ | <tr> | ||
+ | <td width="100px" height="150px"; bgcolor="#980000" > | ||
+ | <p name=1 align="center"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong" target=_top> | ||
+ | <b><font color="#FFE1E1" size=3>Home</font></b> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td width="382px" bgcolor="#CCFF99" valign="baseline"> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="green">Our Project</font></b></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a><font color="green"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="green">Experiments and Results</font></b></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html" target=_top>Strain Construction</a><font color="green"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/mic.html" target=_top>Culture Test</a><font color="green"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/modeling.html" target=_top>Modeling</a><br></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="green">Miscellaneous</font></b></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a></p> | ||
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+ | </td> | ||
+ | <td width="302px" bgcolor="#D09C00" valign="baseline"> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="#FFF4D0">iGEM Resources</font></b></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="#FFF4D0">The Team</font></b></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/team.html" target=_top>iGEM Member List</a><font color="#FFF4D0"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/contribution.html" target=_top>Contributions</a><br></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="#FFF4D0">Achievements</font></b></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements</a><font color="#FFF4D0"> | </font> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <b><font color="#FFF4D0">BioBricks</font></b></p> | ||
+ | <p align="center" valign="baseline"> | ||
+ | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a><br></p> | ||
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- | < | + | <td width="180px"bgcolor="#980000"valign="baseline"> |
- | < | + | <p align="center" valign="baseline"> |
- | < | + | <b><font color="#FFE0E0">Human Practice</font></b></p> |
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- | < | + | <p align="center" valign="baseline"> |
- | < | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a><font color="white"> | </font> |
- | + | <a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a><br></p> | |
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</table> | </table> | ||
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</body> | </body> | ||
</html> | </html> |
Latest revision as of 04:00, 29 October 2011
|
Week 1 (4th-10th June)
Week 2 (13th-17th June)
Culture Test
Week 3 (20th-24th June)
Culture Test
Week 4 (27th June–1st July)
Culture Test
Week 5 (8th-12th July)
Culture Test
Week 6 (15th-19th July)
Culture Test
Week 7 (22nd-26th July)
Culture Test
Week 8 (1st-5th Aug)
Culture Test
Week 9 (8th-12th Aug)
Culture Test
Week 10 (15th-19th Aug)
Culture Test
Week 11 (22nd-26th Aug)
Culture Test
Week 12 (29th Aug-1st Sep)
Culture Test
Week 13 (5th-9th Sep)
Culutre Test
Week 14 (13th-17th Sep)
Culture Test
Week 15 (20th-24th Sep)
Week 16 (27th-30th Sep)
|
Our Project Experiments and Results
Strain Construction |
Culture Test |
Modeling Miscellaneous |
iGEM Resources The Team
iGEM Member List |
Contributions Achievements
Medal Requirements |
BioSafety BioBricks |
Human Practice |