Team:HKUST-Hong Kong/data.html

From 2011.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 8: Line 8:
</style>
</style>
-
 
-
 
<style type="text/css">
<style type="text/css">
<!--
<!--
-
A:link { COLOR: black; TEXT-DECORATION: none; font-weight: normal }
+
A:link { COLOR: black; font-weight: normal }
-
A:visited { COLOR: black; TEXT-DECORATION: none; font-weight: normal }
+
A:visited { COLOR: black; font-weight: normal }
 +
 
 +
A:active { COLOR: black;}
 +
 
 +
A:hover { COLOR: black; font-weight: normal ; text-decoration:underline}
-
A:active { COLOR: black; TEXT-DECORATION: none }
 
-
A:hover { COLOR: black; TEXT-DECORATION: none; font-weight: none; text-decoration: underline; }
 
-->
-->
p{
p{
-
margin: 20px 20px 20px 20px
+
font-family: Georgia, Helvetica, Arial, New Times Roman;
 +
font-size:14;
 +
line-height: 18pt;
 +
font-weight:normal;
 +
margin: 0px 20px 0px 20px;
 +
text-align: justify;
}
}
 +
table{
table{
margin: 0px 0px 0px 0px  
margin: 0px 0px 0px 0px  
}
}
</style>
</style>
-
 
-
 
-
 
</head>
</head>
-
 
-
 
-
<body bgcolor="#9CC3B1">
 
<table style="border-collapse: collapse" width=963>
<table style="border-collapse: collapse" width=963>
<TR bgcolor="#9CC3B1"><td>
<TR bgcolor="#9CC3B1"><td>
-
<a name=bio></a>
+
 
<p align="center"><font face="Verdana, Arial, Helvetica, sans-serif" size="7" color="white">  
<p align="center"><font face="Verdana, Arial, Helvetica, sans-serif" size="7" color="white">  
-
<br>Data Page<br></p>
+
<br>Data Page</font></p>
 +
 
 +
 
 +
<br>
Line 53: Line 56:
<TR bgcolor="white"><td>
<TR bgcolor="white"><td>
-
<center><img src=https://static.igem.org/mediawiki/2011/6/64/Ust_Merge.jpg width=950 height=1096></center><br>
+
<center>
 +
<a href=https://static.igem.org/mediawiki/2011/6/64/Ust_Merge.jpg>
 +
<img src=https://static.igem.org/mediawiki/2011/6/64/Ust_Merge.jpg width=950 height=1096>
 +
</a>
 +
<br>
 +
<font size=2 color=gray>Click to enlarge</font>
 +
</center>
Line 59: Line 68:
<blockquote>
<blockquote>
-
1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> – oriR101 & repA101-ts: This heat sensitive origin of replication origin has been shown to be function at 30°C Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C, <br><br>
+
1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> – oriR101 & repA101-ts: This heat- sensitive origin of replication has been shown to be functional at 30°C. Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.<br><br>
</blockquote>
</blockquote>
Line 87: Line 96:
</font>
</font>
-
<a href=#bio>
 
-
</td></tr></table>
+
 
-
+
</TH>
-
<br>
+
 
 +
 
 +
 
 +
</TD>
 +
  </TR>
 +
 
 +
</table>
 +
 
Line 106: Line 121:
<tr>  
<tr>  
<td width="100px" height="150px"; bgcolor="#980000" >  
<td width="100px" height="150px"; bgcolor="#980000" >  
-
<p align="center"><b><font face="Verdana, Arial, Helvetica, sans-serif" size="2" color="#FFE1E1">  
+
<p align="center">  
-
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong" target=_top><font face="Verdana, Arial, Helvetica, sans-serif" size="4" color="#FFE1E1" font color=white><span style="font-weight:700">Home</span></font></a></font></b></p>
+
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong" target=_top>
 +
<b><font color="#FFE1E1" size=3>Home</font></b>
</p>
</p>
</td>
</td>
-
<td width="332px" bgcolor="#CCFF99" valign="baseline">  
+
<td width="382px" bgcolor="#CCFF99" valign="baseline">  
-
<p align="center" valign="baseline"><b> <font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="green">
+
<p align="center" valign="baseline">
 +
<b><font color="green">Our Project</font></b></p>
 +
 
 +
<p align="center" valign="baseline">
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a><font color="green"> | </font>
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br></p>
 +
 
 +
<p align="center" valign="baseline">
 +
<b><font color="green">Experiments and Results</font></b></p>
 +
 
 +
<p align="center" valign="baseline">
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html"  target=_top>Strain Construction</a><font color="green"> | </font>
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/mic.html"  target=_top>Culture Tests</a><font color="green"> | </font>
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/modeling.html"  target=_top>Modeling</a><br></p>
 +
 
 +
<p align="center" valign="baseline">
 +
<b><font color="green">Miscellaneous</font></b></p>
 +
 
 +
<p align="center" valign="baseline">
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a></p>
-
Our Project</font></b></p>
 
-
<p align="left"><font size="2" face="Verdana, Arial, Helvetica, sans-serif" color="green">
 
-
<a href="team.html" target=_top><font color=green>
 
-
<a href="overview.html" target=_top>Overview</a> |
 
-
<a href="data.html" target=_top>Data Page</a><br>
 
-
<span style="line-height:1; font-weight:600">Experiments and Results</span><br>
 
-
<a href="asm.html"  target=_top>Strain Construction</a> |
 
-
<a href="mic.html"  target=_top>Culture Tests</a> |
 
-
<a href="modeling.html"  target=_top>Modeling</a><br>
 
-
<span style="line-height:1; font-weight:600">Miscellaneous</span><br>
 
-
<a href="notebook.html" target=_top>Notebook</a>
 
-
</font></a><br></font></p>
 
</td>
</td>
-
<td width="332px" bgcolor="#D09C00" valign="baseline">
 
-
<p align="left"><b><font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="#FFF4D0">
 
-
         
 
-
iGEM Resources</font></b></p>
 
-
<p align="left"><font face="Verdana, Arial, Helvetica, sans-serif" size="2" color="#FFFFFF">
 
-
<a href="acknowledgement.html" target=_top>Acknowledgements</a><br>
 
-
<span style="line-height:0.7; font-weight:600">The Team</span><br>
 
-
<a href="team.html" target=_top>iGEM Member List</a> |
 
-
<a href="contribution.html" target=_top>Contributions</a><br>
 
-
<span style="line-height:0.7; font-weight:600">Achievements</span><br>
 
-
<a href="medal.html" target=_top>Medal Requirements</a> |
 
-
<a href="biosafety.html" target=_top>BioSafety</a><br>
 
-
<span style="line-height:0.7; font-weight:600">BioBricks</span><br>
 
-
<a href="characterization.html" target=_top>Master List & Characterization Data</a>
 
 +
<td width="302px" bgcolor="#D09C00" valign="baseline">
 +
<p align="center" valign="baseline">
 +
<b><font color="#FFF4D0">iGEM Resources</font></b></p>
 +
 +
<p align="center" valign="baseline">
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a></p>
 +
 +
 +
<p align="center" valign="baseline">
 +
<b><font color="#FFF4D0">The Team</font></b></p>
 +
 +
<p align="center" valign="baseline">
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/team.html" target=_top>iGEM Member List</a><font color="#FFF4D0"> | </font>
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/contribution.html" target=_top>Contributions</a><br></p>
 +
 +
 +
<p align="center" valign="baseline">
 +
<b><font color="#FFF4D0">Achievements</font></b></p>
 +
 +
<p align="center" valign="baseline">
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements<font color="#FFF4D0"> | </font>
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p>
 +
 +
 +
<p align="center" valign="baseline">
 +
<b><font color="#FFF4D0">BioBricks</font></b></p>
 +
 +
 +
<p align="center" valign="baseline">
 +
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a><br></p>
-
<a href=.html><font color=white>
 
-
</font></a><br><font></p>
 
</td>
</td>
-
<td width="200px"bgcolor="#980000"valign="baseline">  
+
<td width="180px"bgcolor="#980000"valign="baseline">  
-
<p align="left" valign="baseline"><b><font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="#FFE0E0">
+
<p align="center" valign="baseline">
-
Human Practice</font></b></p>
+
<b><font color="#FFE0E0">Human Practice</font></b></p>
-
<p align="left"><font face="Verdana, Arial, Helvetica, sans-serif" size="2" color="#FFFFFF">
+
 
-
<a href="workshop.html" target=_top>Workshop</a> |
+
<p align="center" valign="baseline">
-
<font face="Verdana, Arial, Helvetica, sans-serif" size="2" color="#FFFFFF">
+
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a><font color="white"> | </font>
-
<a href="survey.html" target=_top>Survey</a>
+
<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a><br></p>
 +
 
 +
 
-
</span></p>
 
</td>
</td>

Latest revision as of 18:22, 28 October 2011


Data Page




Click to enlarge
  • Data For Our Favorite New Parts:
1. BBa_K524000 – oriR101 & repA101-ts: This heat- sensitive origin of replication has been shown to be functional at 30°C. Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.

  • Parts Constructed But Not Characterized
1. BBa_K524001 – pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.

2. BBa_K524002 – pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.

3. BBa_K524003 – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD+ synthetase.

4. BBa_K524004 – pir gene: the pir gene encodes the autogenously regulated pi protein.

5. BBa_K524005 – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.

6. BBa_K524006 – RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.

7. BBa_K524007 – RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.

8. BBa_K524100 – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.

Home

Our Project

Overview | Data Page

Experiments and Results

Strain Construction | Culture Tests | Modeling

Miscellaneous

Notebook

iGEM Resources

Acknowledgements

The Team

iGEM Member List | Contributions

Achievements

Medal Requirements | BioSafety

BioBricks

Master List & Characterization Data

Human Practice

Workshop | Survey

Retrieved from "http://2011.igem.org/Team:HKUST-Hong_Kong/data.html"