Team:HKUST-Hong Kong/data.html

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<br>Data Page<br></p>
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1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> –oriR101 & repA101-ts: This heat sensitive origin of replication origin has been shown to be function at 30°C Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C, <br><br>
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1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> – oriR101 & repA101-ts: This heat- sensitive origin of replication has been shown to be functional at 30°C. Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.<br><br>
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1. <a href=http://partsregistry.org/Part:BBa_K524001><font color=blue>BBa_K524001</font></a> –pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.<br><br>
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1. <a href=http://partsregistry.org/Part:BBa_K524001><font color=blue>BBa_K524001</font></a> – pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.<br><br>
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2. <a href=http://partsregistry.org/Part:BBa_K524002><font color=blue>BBa_K524002</font></a> –pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.<br><br>
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2. <a href=http://partsregistry.org/Part:BBa_K524002><font color=blue>BBa_K524002</font></a> – pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.<br><br>
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3. <a href=http://partsregistry.org/Part:BBa_K524003><font color=blue>BBa_K524003</font></a> – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD+ synthatase.<br><br>
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3. <a href=http://partsregistry.org/Part:BBa_K524003><font color=blue>BBa_K524003</font></a> – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD<sup>+</sup> synthetase.<br><br>
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4. <a href=http://partsregistry.org/Part:BBa_K524004><font color=blue>BBa_K524004</font></a> –pir gene: the pir gene encodes the autogenously regulated pi protein.<br><br>
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4. <a href=http://partsregistry.org/Part:BBa_K524004><font color=blue>BBa_K524004</font></a> – pir gene: the pir gene encodes the autogenously regulated pi protein.<br><br>
5. <a href=http://partsregistry.org/Part:BBa_K524005><font color=blue>BBa_K524005</font></a> – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br>
5. <a href=http://partsregistry.org/Part:BBa_K524005><font color=blue>BBa_K524005</font></a> – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br>
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6. <a href=http://partsregistry.org/Part:BBa_K524006><font color=blue>BBa_K524006</font></a> –RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.<br><br>
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6. <a href=http://partsregistry.org/Part:BBa_K524006><font color=blue>BBa_K524006</font></a> – RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.<br><br>
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7. <a href=http://partsregistry.org/Part:BBa_K524007><font color=blue>BBa_K524007</font></a> –RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.<br><br>
+
7. <a href=http://partsregistry.org/Part:BBa_K524007><font color=blue>BBa_K524007</font></a> – RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.<br><br>
8. <a href=http://partsregistry.org/Part:BBa_K524100><font color=blue>BBa_K524100</font></a> – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.<br><br>
8. <a href=http://partsregistry.org/Part:BBa_K524100><font color=blue>BBa_K524100</font></a> – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.<br><br>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong" target=_top><font face="Verdana, Arial, Helvetica, sans-serif" size="4" color="#FFE1E1" font color=white><span style="font-weight:700">Home</span></font></a></font></b></p>
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<p align="center" valign="baseline"><b> <font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="green">
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<b><font color="green">Our Project</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a><font color="green"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br></p>
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<b><font color="green">Experiments and Results</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html"  target=_top>Strain Construction</a><font color="green"> | </font>
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<b><font color="green">Miscellaneous</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a></p>
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Our Project</font></b></p>
 
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<span style="line-height:1; font-weight:600">Experiments and Results</span><br>
 
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<span style="line-height:1; font-weight:600">Miscellaneous</span><br>
 
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iGEM Resources</font></b></p>
 
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<a href="acknowledgement.html" target=_top>Acknowledgements</a><br>
 
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<a href="characterization.html" target=_top>Master List & Characterization Data</a>
 
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<td width="302px" bgcolor="#D09C00" valign="baseline">
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<b><font color="#FFF4D0">iGEM Resources</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a></p>
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<b><font color="#FFF4D0">The Team</font></b></p>
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<b><font color="#FFF4D0">Achievements</font></b></p>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements<font color="#FFF4D0"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p>
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<b><font color="#FFF4D0">BioBricks</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a><br></p>
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Human Practice</font></b></p>
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Latest revision as of 18:22, 28 October 2011


Data Page




Click to enlarge
  • Data For Our Favorite New Parts:
1. BBa_K524000 – oriR101 & repA101-ts: This heat- sensitive origin of replication has been shown to be functional at 30°C. Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.

  • Parts Constructed But Not Characterized
1. BBa_K524001 – pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.

2. BBa_K524002 – pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.

3. BBa_K524003 – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD+ synthetase.

4. BBa_K524004 – pir gene: the pir gene encodes the autogenously regulated pi protein.

5. BBa_K524005 – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.

6. BBa_K524006 – RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.

7. BBa_K524007 – RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.

8. BBa_K524100 – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.

Home

Our Project

Overview | Data Page

Experiments and Results

Strain Construction | Culture Tests | Modeling

Miscellaneous

Notebook

iGEM Resources

Acknowledgements

The Team

iGEM Member List | Contributions

Achievements

Medal Requirements | BioSafety

BioBricks

Master List & Characterization Data

Human Practice

Workshop | Survey

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