Team:Tsinghua-A/Parts

From 2011.igem.org

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h1 {
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/* Menu */
/* Menu */
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</head>
<body>
<body>
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<p align="CENTER"><img src="https://static.igem.org/mediawiki/2011/1/17/THU_banner_par.jpg" alt="" width="960"/></p>
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<div style="position:fixed;right:0px;bottom: 0px;display:inline"><a href="#back-top">
 +
<img src="https://static.igem.org/mediawiki/2011/5/52/Back-top.png" width="60px" height="60px"></a></div>
 +
<p id="back-top" align="CENTER" STYLE="text-indent:0em"><img src="https://static.igem.org/mediawiki/2011/1/17/THU_banner_par.jpg" alt="" width="960"/></p>
<div id="menu">
<div id="menu">
<ul>
<ul>
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</ul>
</ul>
</div>
</div>
-
<br>
+
 
-
<P><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US">Parts
+
-
Section</SPAN></FONT></FONT></P>
+
-
<br>
+
<!--end of enu--------------------------------------------><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
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<HTML>
<HTML>
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</HEAD>
</HEAD>
<BODY LANG="zh-CN" DIR="LTR">
<BODY LANG="zh-CN" DIR="LTR">
-
<br>
+
<br><br>
-
<P ALIGN=LEFT><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 14pt"><SPAN LANG="en-US">Parts
+
<div class="nav">
-
List</SPAN></FONT></FONT></P>
+
<table id="toc" class="toc">
-
<P><Br></P>
+
<tr>
-
 
+
<td>
-
<TABLE WIDTH=939 BORDER=0 CELLPADDING=0 CELLSPACING=0 BGCOLOR="#ccccff">
+
<div id="toctitle">
-
<COL WIDTH=196>
+
<h2>Contents</h2>
-
<COL WIDTH=165>
+
</div>
-
<COL WIDTH=415>
+
<ul>
-
<COL WIDTH=163>
+
<li class="toclevel-1 tocsection-1"><a href="#Parts List"><span class="tocnumber">1</span> <span class="toctext">Parts List</span></a></li>
-
<TR>
+
<li class="toclevel-1 tocsection-2"><a href="#Descriptions"><span class="tocnumber">2</span> <span class="toctext">Descriptions</span></a></li>
-
<TH WIDTH=196 BGCOLOR="#aaaaff">
+
<li class="toclevel-1 tocsection-3"><a href="#Methods"><span class="tocnumber">3</span> <span class="toctext">Methods</span></a></li>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Name</SPAN></FONT></FONT></P>
+
<li class="toclevel-1 tocsection-4"><a href="#Results"><span class="tocnumber">4</span> <span class="toctext">Results</span></a></li>
-
</TH>
+
</ul>
-
<TH WIDTH=165 BGCOLOR="#aaaaff">
+
</td>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Type</SPAN></FONT></FONT></P>
+
</tr>
-
</TH>
+
</table>
-
<TH WIDTH=415 BGCOLOR="#aaaaff">
+
</div>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Description</SPAN></FONT></FONT></P>
+
<br><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
-
</TH>
+
<HTML>
-
<TH WIDTH=163 BGCOLOR="#aaaaff">
+
<HEAD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Length</SPAN></FONT></FONT></P>
+
<META HTTP-EQUIV="CONTENT-TYPE" CONTENT="text/html; charset=gb2312">
-
</TH>
+
<TITLE></TITLE>
-
</TR>
+
<META NAME="GENERATOR" CONTENT="OpenOffice.org 3.2  (Win32)">
-
<TR>
+
<META NAME="AUTHOR" CONTENT="Sl Y">
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
<META NAME="CREATED" CONTENT="20111028;10190100">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574000"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574000</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
<META NAME="CHANGEDBY" CONTENT="Sl Y">
-
</TD>
+
<META NAME="CHANGED" CONTENT="20111028;10224900">
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
</HEAD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
<BODY LANG="zh-CN" DIR="LTR">
-
</TD>
+
<H1 id="Parts List"><A NAME="Parts List"></A><FONT COLOR="#006b6b"><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US"><B>Parts
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
List</B></SPAN></FONT></FONT></FONT></H1>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">pLux + TetR</SPAN></FONT></FONT></P>
+
<HR SIZE=1>
-
</TD>
+
<P STYLE="margin-bottom: 0cm; line-height: 0.58cm; widows: 2; orphans: 2">
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
<BR>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">903</SPAN></FONT></FONT></P>
+
</P>
-
</TD>
+
<CENTER>
-
</TR>
+
<TABLE WIDTH=760 BORDER=4 CELLPADDING=0 CELLSPACING=0 BGCOLOR="#ffffff">
-
<TR>
+
<COL WIDTH=160>
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
<COL WIDTH=130>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574001"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574001</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
<COL WIDTH=340>
-
</TD>
+
<COL WIDTH=130>
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
<TR>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
<TH WIDTH=160 BGCOLOR="#aaaaff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Name</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
</TH>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">TetR
+
<TH WIDTH=130 BGCOLOR="#aaaaff">
-
regulated by 3OC6HSL</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Type</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TH>
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
<TH WIDTH=340 BGCOLOR="#aaaaff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">1890</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Description</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TH>
-
</TR>
+
<TH WIDTH=130 BGCOLOR="#aaaaff">
-
<TR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Length</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
</TH>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574002"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574002</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
</TR>
-
</TD>
+
<TR>
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574000"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574000</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">pluxR
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
+ CFP</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pLux
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">941</SPAN></FONT></FONT></P>
+
+ TetR</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
</TR>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">903</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574003"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574003</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
</TR>
-
</TD>
+
<TR>
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574001"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574001</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">3OC6HSL -> TetR && reporter</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">2839</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">TetR
-
</TD>
+
regulated by 3OC6HSL</SPAN></FONT></FONT></FONT></P>
-
</TR>
+
</TD>
-
<TR>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1890</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574004"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574004</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
</TD>
-
</TD>
+
</TR>
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
<TR>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574002"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574002</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">3OC12HSL regulated by TetR</SPAN></FONT></FONT></P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">797</SPAN></FONT></FONT></P>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pluxR
-
</TR>
+
+ CFP</SPAN></FONT></FONT></FONT></P>
-
<TR>
+
</TD>
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574005"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574005</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">941</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
</TR>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
<TR>
-
</TD>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574003"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574003</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">3OC12HSL and YFP regulated by pBad</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">1712</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
</TR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC6HSL
-
<TR>
+
-&gt; TetR &amp;&amp; reporter</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574006"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574006</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2839</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P>
+
</TR>
-
</TD>
+
<TR>
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">3OC12HSL regulated by pTet and pBad</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574004"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574004</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">2517</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
</TR>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<TR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
regulated by TetR</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574007"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574007</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">797</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
</TR>
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
<TR>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">lasR + TT</SPAN></FONT></FONT></P>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574005"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574005</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">918</SPAN></FONT></FONT></P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
</TR>
+
</TD>
-
<TR>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574008"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574008</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
and YFP regulated by pBad</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1712</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
</TR>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">a constitutive LasR producer</SPAN></FONT></FONT></P>
+
<TR>
-
</TD>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574006"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574006</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">982</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></FONT></P>
-
<TR>
+
</TD>
-
<TD WIDTH=196 BGCOLOR="#ddddff">
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">BBa_K574009</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL
-
</TD>
+
regulated by pTet and pBad</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=165 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2517</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=415 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">3OC12HSL -> PoPS Receiver</SPAN></FONT></FONT></P>
+
</TR>
-
</TD>
+
<TR>
-
<TD WIDTH=163 BGCOLOR="#ddddff">
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">1147</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574007"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574007</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
</TD>
+
</TD>
-
</TR>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TABLE>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=CENTER><BR><BR>
+
</TD>
-
</P><P><FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Description:</SPAN></FONT></FONT></FONT></P>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<P ALIGN=LEFT STYLE="margin-left: 1.02cm; line-height: 0.58cm; widows: 2; orphans: 2">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">lasR
-
<FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">We
+
+ TT</SPAN></FONT></FONT></FONT></P>
-
assembled&nbsp;</SPAN></SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_E0840</SPAN></SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;under
+
</TD>
-
the pLas promoter&nbsp;</SPAN></SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_R0079"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_R0079</SPAN></SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;that
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
was contained into K574009. We kept pSB1A2 as the scaffold vector.</SPAN></SPAN></SPAN></FONT></FONT></FONT></SPAN></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">918</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=LEFT STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=4><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">Methods:</SPAN></SPAN></SPAN></FONT></FONT></FONT></SPAN></FONT></P>
+
</TD>
-
<P ALIGN=LEFT STYLE="margin-left: 1.19cm; line-height: 0.58cm; widows: 2; orphans: 2">
+
</TR>
-
<FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">1.
+
<TR>
-
Ligate&nbsp;</SPAN></SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_E0840</SPAN></SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;downstream
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
of&nbsp;</SPAN></SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_K574009</SPAN></SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">,
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574008"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574008</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
which acts as a reporter.<BR>2. Culture the positive colony at 37&deg;C,
+
</TD>
-
220 rpm for 12 hours, as well as the DH5alpha.<BR>3. Dilute 1:25 the
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
former in 12 falcon tubes (4 ml cultures), each 3 tubes as a group.
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
The later is also diluted in 3 tubes as the negative control.<BR>4.
+
</TD>
 +
<TD WIDTH=340 BGCOLOR="#ddddff">
 +
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">a
 +
constitutive LasR producer</SPAN></FONT></FONT></FONT></P>
 +
</TD>
 +
<TD WIDTH=130 BGCOLOR="#ddddff">
 +
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">982</SPAN></FONT></FONT></FONT></P>
 +
</TD>
 +
</TR>
 +
<TR>
 +
<TD WIDTH=160 BGCOLOR="#ddddff">
 +
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574009</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
 +
</TD>
 +
<TD WIDTH=130 BGCOLOR="#ddddff">
 +
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
 +
</TD>
 +
<TD WIDTH=340 BGCOLOR="#ddddff">
 +
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL
 +
-&gt; PoPS Receiver</SPAN></FONT></FONT></FONT></P>
 +
</TD>
 +
<TD WIDTH=130 BGCOLOR="#ddddff">
 +
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1147</SPAN></FONT></FONT></FONT></P>
 +
</TD>
 +
</TR>
 +
</TABLE>
 +
</CENTER>
 +
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
 +
</P>
 +
<H1 id="Descriptions" STYLE="page-break-before: always; page-break-after: avoid"><A NAME="Descriptions"></A>
 +
<FONT COLOR="#006b6b"><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US">Descriptions</SPAN></FONT></FONT></FONT></H1>
 +
<HR SIZE=1>
 +
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
 +
</P>
 +
<P STYLE="margin-left: 1.13cm; line-height: 200%; widows: 2; orphans: 2">
 +
<FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">We
 +
assembled&nbsp;</SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_E0840</SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;under
 +
the pLas promoter&nbsp;</SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_R0079"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_R0079</SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;that
 +
was contained into K574009. We kept pSB1A2 as the scaffold vector.</SPAN></SPAN></FONT></FONT></FONT></SPAN></FONT></P>
 +
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
 +
</P>
 +
<H1 id="Methods" STYLE="page-break-before: always; page-break-after: avoid"><A NAME="Methods"></A>
 +
<FONT COLOR="#006b6b"><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US">Methods</SPAN></FONT></FONT></FONT></H1>
 +
<HR SIZE=1>
 +
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
 +
</P>
 +
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
 +
<FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">1.
 +
Ligate&nbsp;</SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_E0840</SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;downstream
 +
of&nbsp;</SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_K574009</SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;,
 +
which acts as a reporter.</SPAN></SPAN></FONT></FONT></FONT></SPAN></FONT></P>
 +
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
 +
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">2.
 +
Culture the positive colony at 37&deg;C, 220 rpm for 12 hours, as
 +
well as the DH5alpha.</SPAN></FONT></FONT></FONT></P>
 +
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
 +
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">3.
 +
Dilute 1:25 the former in 15 falcon tubes (4 ml cultures), each 3
 +
tubes as a group. The later is also diluted in 3 tubes as the
 +
negative control.</SPAN></FONT></FONT></FONT></P>
 +
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
 +
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">4.
After culturing for another 2 hours, as their OD600 reach 0.2, induce
After culturing for another 2 hours, as their OD600 reach 0.2, induce
-
with 3OC12HSL 0, 1e-6 M, 1e-5 M and 1e-4 M respectively.<BR>5. Take
+
with 3OC12HSL 0, 1e-7 M, 1e-6 M, 1e-5 M and 1e-4 M respectively.</SPAN></FONT></FONT></FONT></P>
-
samples after 1 hour, 4 hours, 8 hours and 24 hours, that is,
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
 +
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">5.
 +
Take samples after 1 hour, 4 hours, 8 hours and 24 hours, that is,
centrifugalizate 0.5 mL of each culture and suspend them by 1 mL of
centrifugalizate 0.5 mL of each culture and suspend them by 1 mL of
-
PBS (phosphate-buffered saline).<BR>6. Measure the fluorescence of
+
PBS (phosphate-buffered saline).</SPAN></FONT></FONT></FONT></P>
-
the samples with the flow cytometer.</SPAN></SPAN></SPAN></FONT></FONT></FONT></SPAN></FONT></P>
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
-
<P STYLE="margin-left: 1.19cm"><BR><BR>
+
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">6.
 +
Measure the fluorescence of the samples with the flow cytometer.</SPAN></FONT></FONT></FONT></P>
 +
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
</P>
</P>
-
 
+
<H1 id="Results" STYLE="page-break-before: always; page-break-after: avoid"><A NAME="Results"></A>
-
<p align="CENTER"><img src="https://static.igem.org/mediawiki/2011/c/ca/K574009_chart.png" NAME="K574009_chart" /></p>
+
<FONT COLOR="#006b6b"><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US">Results</SPAN></FONT></FONT></FONT></H1>
-
 
+
<HR SIZE=1>
-
<p align="CENTER"><img src="https://static.igem.org/mediawiki/2011/d/da/K574009_img.jpg" /></p>
+
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
-
 
+
</P>
-
<P><FONT COLOR="#000000"><FONT FACE="Arial, Helvetica, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Results:</SPAN></FONT></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="line-height: 200%; widows: 2; orphans: 2"><FONT COLOR="#000000"><IMG SRC="https://static.igem.org/mediawiki/2011/c/ca/K574009_chart.png" NAME="K574009_chart" ALIGN=BOTTOM WIDTH=770 HEIGHT=490 BORDER=0></FONT></P>
-
<P ALIGN=LEFT STYLE="margin-left: 1.19cm; line-height: 0.58cm; widows: 2; orphans: 2">
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<P ALIGN=CENTER STYLE="line-height: 200%; widows: 2; orphans: 2"><BR><BR>
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<FONT COLOR="#000000"><FONT FACE="Arial Unicode MS, sans-serif"><FONT SIZE=3><SPAN LANG="en-US">From
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</P>
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<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
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<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">From
the chart, we can see that cells were hardly induced in the control
the chart, we can see that cells were hardly induced in the control
group, and with the concentration of inducer growing, the intensity
group, and with the concentration of inducer growing, the intensity
of GFP increased by groups. The most efficient concentration of
of GFP increased by groups. The most efficient concentration of
-
inducer was around 10^-6M, and to most groups, the intensity of GFP
+
inducer was around 10<SUP>-5</SUP>M, and higher concentration may
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reached its maxium after 4 hours.</SPAN></FONT></FONT></FONT></P>
+
lead to the expression of GFP decreasing. Additionally, to most
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+
groups, the intensity of GFP reached its maximum after 4 hours.</SPAN></FONT></FONT></FONT></P>
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<p align="CENTER"><a href="https://2011.igem.org/Team:Tsinghua-A"><img src="https://static.igem.org/mediawiki/2011/9/92/Killbanner_header.jpg" alt="" width="960"/><a href="https://2011.igem.org"><img src="https://static.igem.org/mediawiki/igem.org/2/29/Killbanner_header2.jpg" alt="" width="960"/></p>
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Latest revision as of 02:28, 28 October 2011

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Contents

Parts List


Name

Type

Description

Length

BBa_K574000

Composite

pLux + TetR

903

BBa_K574001

Composite

TetR regulated by 3OC6HSL

1890

BBa_K574002

Composite

pluxR + CFP

941

BBa_K574003

Device

3OC6HSL -> TetR && reporter

2839

BBa_K574004

Composite

3OC12HSL regulated by TetR

797

BBa_K574005

Composite

3OC12HSL and YFP regulated by pBad

1712

BBa_K574006

Device

3OC12HSL regulated by pTet and pBad

2517

BBa_K574007

Composite

lasR + TT

918

BBa_K574008

Composite

a constitutive LasR producer

982

BBa_K574009

Composite

3OC12HSL -> PoPS Receiver

1147



Descriptions



We assembled BBa_E0840 under the pLas promoter BBa_R0079 that was contained into K574009. We kept pSB1A2 as the scaffold vector.



Methods



1. Ligate BBa_E0840 downstream of BBa_K574009 , which acts as a reporter.

2. Culture the positive colony at 37°C, 220 rpm for 12 hours, as well as the DH5alpha.

3. Dilute 1:25 the former in 15 falcon tubes (4 ml cultures), each 3 tubes as a group. The later is also diluted in 3 tubes as the negative control.

4. After culturing for another 2 hours, as their OD600 reach 0.2, induce with 3OC12HSL 0, 1e-7 M, 1e-6 M, 1e-5 M and 1e-4 M respectively.

5. Take samples after 1 hour, 4 hours, 8 hours and 24 hours, that is, centrifugalizate 0.5 mL of each culture and suspend them by 1 mL of PBS (phosphate-buffered saline).

6. Measure the fluorescence of the samples with the flow cytometer.



Results





From the chart, we can see that cells were hardly induced in the control group, and with the concentration of inducer growing, the intensity of GFP increased by groups. The most efficient concentration of inducer was around 10-5M, and higher concentration may lead to the expression of GFP decreasing. Additionally, to most groups, the intensity of GFP reached its maximum after 4 hours.




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