Team:HKU-Hong Kong/Parts
From 2011.igem.org
GeorgeLeung (Talk | contribs) |
|||
(6 intermediate revisions not shown) | |||
Line 3: | Line 3: | ||
|style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;"|Sample Data Page | |style="font-family: georgia, helvetica, arial, sans-serif;font-size:2em;color:#01DF01;"|Sample Data Page | ||
|- | |- | ||
- | |style="width: | + | |style="width:900px;"| |
<div ALIGN=CENTER> | <div ALIGN=CENTER> | ||
{| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5"; | {| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5"; | ||
Line 15: | Line 15: | ||
|- | |- | ||
|style="width:900px;"| | |style="width:900px;"| | ||
- | By expressing the tetR | + | By expressing the tetR-HNS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the 2 tetR binding region (tetO2).Thus in our designed operator- repressor binding, the presence of 2 tetR binding sites are used to enhance the binding of tetR part of the fusion proteins. As the fusion proteins bound to tetO, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved. |
+ | |- | ||
+ | |style="width:900px;"| | ||
+ | <div ALIGN=CENTER> | ||
+ | {| style="width:254px;background:#99EE63;text-align:center;font-family: georgia, helvetica, arial, sans-serif;color:#000000;margin- top:5px;padding: 2px;" cellspacing="5"; | ||
+ | |- | ||
+ | |[[Image:Sample Data Table.png|650px]] | ||
+ | |- | ||
+ | |} | ||
+ | </div> | ||
+ | |} | ||
+ | {| style="width:900px;background:#000000;text-align:justify;font-family: georgia, helvetica, arial, sans-serif;color:#ffffff;margin-top:25px;" cellspacing="20" | ||
+ | |- | ||
+ | |style="width:900px;"| | ||
+ | '''Experience:''' | ||
+ | We have entered the confirmation data for the characterization of the selected constitutive promoters we have used. Of the 4 promoters we have used in the project, our team considered that J23109 or J23116 are more suitable promoters in creating the super silencer repression system. | ||
- | + | These parts are: | |
- | + | ||
- | + | ||
{| style="width:900px;background:#000000;text-align:justify;font-family: georgia, helvetica, arial, sans-serif;color:#ffffff;margin-top:25px;" cellspacing="20" | {| style="width:900px;background:#000000;text-align:justify;font-family: georgia, helvetica, arial, sans-serif;color:#ffffff;margin-top:25px;" cellspacing="20" | ||
|- | |- | ||
- | |style="font-family: georgia, helvetica, arial, sans-serif;font-size: | + | |style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="left"|[http://partsregistry.org/Part:BBa_J23103:Experience '''BBa_J23103''' ] |
- | |style="font-family: georgia, helvetica, arial, sans-serif;font-size: | + | |style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="centre"|[http://partsregistry.org/Part:BBa_J23106:Experience '''BBa_J23106''' ] |
- | |style="font-family: georgia, helvetica, arial, sans-serif;font-size: | + | |style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="right"|[http://partsregistry.org/Part:BBa_J23109:Experience'''BBa_J23109''' ] |
- | + | |style="font-family: georgia, helvetica, arial, sans-serif;font-size:1.5em;color:#01DF01;" align="right"|[http://partsregistry.org/Part:BBa_J23116:Experience'''BBa_J23116''' ] | |
- | + | ||
- | |style="font-family: georgia, helvetica, arial, sans-serif;font-size: | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
|- | |- | ||
+ | |} |
Latest revision as of 17:30, 5 October 2011
Sample Data Page |
By expressing the tetR-HNS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the 2 tetR binding region (tetO2).Thus in our designed operator- repressor binding, the presence of 2 tetR binding sites are used to enhance the binding of tetR part of the fusion proteins. As the fusion proteins bound to tetO, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved. |
Experience: We have entered the confirmation data for the characterization of the selected constitutive promoters we have used. Of the 4 promoters we have used in the project, our team considered that J23109 or J23116 are more suitable promoters in creating the super silencer repression system. These parts are:
|