Team:HKUST-Hong Kong

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<b>Greetings from the <b>2011 HKUST iGEM Team</b>!</b><br><br>
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Welcome to our Wiki page!<br></p>
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This is the 4<sup>th</sup> time HKUST has participated in this international synthetic biology competition.<br>
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Thanks to our instructors' helpful advice, considerate advisors, and great enthusiasm and efforts from all members of the HKUST iGEM 2011 team, we have enjoyed a fantastic summer working with something that we believe will make a difference. <br><br>
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You may visit our <a href=https://2011.igem.org/Team:HKUST-Hong_Kong/gallery.html target=_top><font color=white><u>Gallery</u></font></a> to see the snapshots of us working in the lab.
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<img style=margin:20px align=left src="https://static.igem.org/mediawiki/2011/1/19/HKUST_Index_logo.jpg" width=120 height=120 align=left">
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<p align="center" valign="baseline" ><b> <font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="green">
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Our Project
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a> |
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br>
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Experiments and Results<br>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html"  target=_top>Strain Construction</a> |
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/mic.html"  target=_top>Culture Tests</a> |
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/modeling.html"  target=_top>Modeling</a><br>
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Miscellaneous<br>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a>
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<img style=margin:20px src=https://static.igem.org/mediawiki/2011/d/d6/HKUST_Igem_resources.jpg width=120 height=120 align=left>
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<p align="center" valign="baseline" ><b> <font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="#D09C00">
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iGEM Resources
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a><br>
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The Team<br>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/team.html" target=_top>iGem Member List</a> |
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/contribution.html" target=_top>Contributions</a><br>
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Achievements<br>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements</a> |
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br>
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BioBricks<br>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a>
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Human Practice
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a> |
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a>
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<b><u>Project Abstract</u></b></font></p><br>
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<font color=#CDD2C2>It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings suggest that communities with a mixture of highly resistant (HR) and less resistant (LR) individuals are able to survive through ‘charity’ by HR individuals, which support LR individuals through indole signalling.</p><br>
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<p>Our team aims to interfere with this signalling through introducing a disruptor <i>E. coli</i> into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO).  We hypothesize that LR cells in the community deprived of indole will undergo elimination at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling.</p><br>
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<p>Along the way, we will also create a new strain of <i>E. coli</i> that utilizes an essential gene (<i>nadE</i>) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol.</p><br><br><br>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong" target=_top>
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<b><font color="#FFE1E1" size=3>Home</font></b>
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<td width="382px" bgcolor="#CCFF99" valign="baseline">
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<b><font color="green">Our Project</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/overview.html" target=_top>Overview</a><font color="green"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/data.html" target=_top>Data Page</a><br></p>
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<p align="center" valign="baseline">
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<b><font color="green">Experiments and Results</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/asm.html"  target=_top>Strain Construction</a><font color="green"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/mic.html"  target=_top>Culture Tests</a><font color="green"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/modeling.html"  target=_top>Modeling</a><br></p>
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<p align="center" valign="baseline">
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<b><font color="green">Miscellaneous</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/notebook.html" target=_top>Notebook</a></p>
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<td width="302px" bgcolor="#D09C00" valign="baseline">
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<p align="center" valign="baseline">
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<b><font color="#FFF4D0">iGEM Resources</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/acknowledgement.html" target=_top>Acknowledgements</a></p>
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<p align="center" valign="baseline">
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<b><font color="#FFF4D0">The Team</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/team.html" target=_top>iGEM Member List</a><font color="#FFF4D0"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/contribution.html" target=_top>Contributions</a><br></p>
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<p align="center" valign="baseline">
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<b><font color="#FFF4D0">Achievements</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/medal.html" target=_top>Medal Requirements</a><font color="#FFF4D0"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/biosafety.html" target=_top>BioSafety</a><br></p>
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<p align="center" valign="baseline">
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<b><font color="#FFF4D0">BioBricks</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/characterization.html" target=_top>Master List & Characterization Data</a><br></p>
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</td>
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<td width="180px"bgcolor="#980000"valign="baseline">
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<b><font color="#FFE0E0">Human Practice</font></b></p>
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<p align="center" valign="baseline">
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/workshop.html" target=_top>Workshop</a><font color="white"> | </font>
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<a href="https://2011.igem.org/Team:HKUST-Hong_Kong/survey.html" target=_top>Survey</a><br></p>
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Latest revision as of 16:49, 27 October 2011


Greetings from the 2011 HKUST iGEM Team!

Welcome to our Wiki page!

This is the 4th time HKUST has participated in this international synthetic biology competition.
Thanks to our instructors' helpful advice, considerate advisors, and great enthusiasm and efforts from all members of the HKUST iGEM 2011 team, we have enjoyed a fantastic summer working with something that we believe will make a difference.

You may visit our Gallery to see the snapshots of us working in the lab.


Our Project

Overview | Data Page
Experiments and Results
Strain Construction | Culture Tests | Modeling
Miscellaneous
Notebook


iGEM Resources

Acknowledgements
The Team
iGem Member List | Contributions
Achievements
Medal Requirements | BioSafety
BioBricks
Master List & Characterization Data


Human Practice

Workshop | Survey

Project Abstract


It has often been assumed that when an antibiotic is introduced to a bacterial community, only cells that carry resistance genes will survive and proliferate. However, recent findings suggest that communities with a mixture of highly resistant (HR) and less resistant (LR) individuals are able to survive through ‘charity’ by HR individuals, which support LR individuals through indole signalling.


Our team aims to interfere with this signalling through introducing a disruptor E. coli into the bacterial community. This new strain will be able to degrade indole using a mutated toluene-4-monooxygenase (T4MO). We hypothesize that LR cells in the community deprived of indole will undergo elimination at lower antibiotic concentrations. If this demonstration is successful, indole degradation might prove to be a possible strategy in boosting antibiotics effectiveness in medical practice against bacteria that rely on such signalling.


Along the way, we will also create a new strain of E. coli that utilizes an essential gene (nadE) as the selection marker for transformation, allowing antibiotics-free transformation and plasmid maintenance for regular laboratory manipulation. This new transformation method can be adopted for future iGEM teams, reducing their use of antibiotics without increasing the complexity of the transformation protocol.

Home

Our Project

Overview | Data Page

Experiments and Results

Strain Construction | Culture Tests | Modeling

Miscellaneous

Notebook

iGEM Resources

Acknowledgements

The Team

iGEM Member List | Contributions

Achievements

Medal Requirements | BioSafety

BioBricks

Master List & Characterization Data

Human Practice

Workshop | Survey

Retrieved from "http://2011.igem.org/Team:HKUST-Hong_Kong"