Team:HKUST-Hong Kong/data.html

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<br>Data Page<br></p>
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1. Main Page –oriR101 & repA101-ts, BBa_K524000: This heat sensitive origin of replication origin has been shown to function only when the incubation temperature is below X°C.<br><br>
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1. <a href=http://partsregistry.org/Part:BBa_K524000><font color=blue>BBa_K524000</font></a> – oriR101 & repA101-ts: This heat- sensitive origin of replication has been shown to be functional at 30°C. Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.<br><br>
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<ul><li><b>We did not characterize any pre-existing part</b></ul></li><br><br>
 
<ul><li><b>Parts Constructed But Not Characterized</b></ul></li>
<ul><li><b>Parts Constructed But Not Characterized</b></ul></li>
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1. Main Page –pLac + RBS + sfGFP1-10, BBa_K524001: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.<br><br>
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1. <a href=http://partsregistry.org/Part:BBa_K524001><font color=blue>BBa_K524001</font></a> – pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.<br><br>
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2. Main Page –pLac + RBS + sfGFP11 + double terminator, BBa_K524002: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.<br><br>
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2. <a href=http://partsregistry.org/Part:BBa_K524002><font color=blue>BBa_K524002</font></a> – pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.<br><br>
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3. Main Page – nadE gene + double terminator, BBa_K524003: an essential gene that encodes constitutive expressed NAD+ synthatase.<br><br>
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3. <a href=http://partsregistry.org/Part:BBa_K524003><font color=blue>BBa_K524003</font></a> – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD<sup>+</sup> synthetase.<br><br>
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4. Main Page –pir gene, BBa_K524004: the pir gene encodes the autogenously regulated pi protein.<br><br>
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4. <a href=http://partsregistry.org/Part:BBa_K524004><font color=blue>BBa_K524004</font></a> – pir gene: the pir gene encodes the autogenously regulated pi protein.<br><br>
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5. Main Page – pToolkit, BBa_K524005: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br>
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5. <a href=http://partsregistry.org/Part:BBa_K524005><font color=blue>BBa_K524005</font></a> – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.<br><br>
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6. Main Page –RBS + sfGFP1-10, BBa_K524006: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.<br><br>
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6. <a href=http://partsregistry.org/Part:BBa_K524006><font color=blue>BBa_K524006</font></a> – RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.<br><br>
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7. Main Page –RBS + sfGFP11 + double terminator, BBa_K524007: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.<br><br>
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7. <a href=http://partsregistry.org/Part:BBa_K524007><font color=blue>BBa_K524007</font></a> – RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.<br><br>
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8. Main Page – bcr CDS + double terminator B0015, BBa_K524100: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degree of resistance to 5 antibiotics.<br><br>
+
8. <a href=http://partsregistry.org/Part:BBa_K524100><font color=blue>BBa_K524100</font></a> – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.<br><br>
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<p align="center" valign="baseline"><b> <font face="Verdana, Arial, Helvetica, sans-serif" size="3" color="green">
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Latest revision as of 18:22, 28 October 2011


Data Page




Click to enlarge
  • Data For Our Favorite New Parts:
1. BBa_K524000 – oriR101 & repA101-ts: This heat- sensitive origin of replication has been shown to be functional at 30°C. Partial loss of plasmid happens at 33°C, and complete loss of plasmid is observed at 37°C.

  • Parts Constructed But Not Characterized
1. BBa_K524001 – pLac + RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034 and pLac BBa_R0010.

2. BBa_K524002 – pLac + RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034 and pLac BBa_R0010, with double terminator B0015 at the end.

3. BBa_K524003 – nadE gene + double terminator: an essential gene that encodes constitutive expressed NAD+ synthetase.

4. BBa_K524004 – pir gene: the pir gene encodes the autogenously regulated pi protein.

5. BBa_K524005 – pToolkit: A plasmid that is only maintained in strains with genotype pir+. The plasmid was modified from another plasmid pKD46, which has been shown to trigger DNA recombination and subsequent gene knockout under induction of arabinose.

6. BBa_K524006 – RBS + sfGFP1-10: This contains coding DNA sequences for superfolder GFP amino residues 1-214 driven by RBS BBa_B0034.

7. BBa_K524007 – RBS + sfGFP11 + double terminator: This contains coding DNA sequences for superfolder GFP amino residues 215-238 driven by RBS BBa_B0034, with double terminator B0015 at the end.

8. BBa_K524100 – bcr CDS + double terminator B0015: The gene product is responsible for multidrug efflux system that pumps out antibiotics. Effect of pump was documented by other researchers: it confers varying degrees of resistance to 5 antibiotics.

Home

Our Project

Overview | Data Page

Experiments and Results

Strain Construction | Culture Tests | Modeling

Miscellaneous

Notebook

iGEM Resources

Acknowledgements

The Team

iGEM Member List | Contributions

Achievements

Medal Requirements | BioSafety

BioBricks

Master List & Characterization Data

Human Practice

Workshop | Survey

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