Team:Kyoto/BindingAssays

From 2011.igem.org

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(Created page with "===Ligation=== #Make 2µL of Mixture (the vector and the insert at 1 : 5-10) and Control (only the vector). #Add 5µL Ligation High, 1µL T4 Kinase, and 7µL MilliQ to create a ...")
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===Ligation===
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{{Kyoto_Foreground|active_page=method}}
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#Make 2µL of Mixture (the vector and the insert at 1 : 5-10) and Control (only the vector).
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{{Kyoto_Background}}
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#Add 5µL Ligation High, 1µL T4 Kinase, and 7µL MilliQ to create a solution.
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{{Kyoto_WikiDesign}}
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#Incubate at 16℃ for 30 min. If the colonies of E.coli transformed with the Control
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===Restrictive Digestion===
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<ol>
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<li>Use EcoRI, XbaI, SpeI, PstI, (NEB)</li>
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<li>Mix the following.
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{|
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|-
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| Sample
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| 5µL
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|-
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| 10xBuffer
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| 1µL
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|-
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| Restriction Enzyme
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| 0.1µL
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|-
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| MilliQ
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| -3.9µL
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|}
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</li>
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<li>Let stand for 2h at 37℃</li>
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</ol>
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Latest revision as of 03:32, 5 October 2011

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