Team:Glasgow/iGEM Diary

From 2011.igem.org

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{{Team:Glasgow/Header}}
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!align="center"|[[Team:Glasgow|Home]]
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<html>
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!align="center"|[[Team:Glasgow/Team|Team]]
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<h1>Video Logs</h1>
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!align="center"|[[Team:Glasgow/iGEM_Diary|iGEM Diary]]
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<p>Want to know how life in the lab is going? Check out our weekly videos!</p>
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Glasgow Official Team Profile]
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!align="center"|[[Team:Glasgow/Project|Project]]
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!align="center"|[[Team:Glasgow/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Glasgow/Modeling|Modeling]]
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!align="center"|[[Team:Glasgow/Notebook|Notebook]]
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!align="center"|[[Team:Glasgow/Safety|Safety]]
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!align="center"|[[Team:Glasgow/Attributions|Attributions]]
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What better way to get to know the team than to have access to daily updates of our conquers and defeats?
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{{#calendar: title=Glasgow |year=2011 | month=06}}
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<h2>Week 1 Summary</h2>
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{{#calendar: title=Glasgow |year=2011 | month=07}}
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This week the video is presented by Michal Przydacz. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Michal_Przydacz>here</a> to find out more about Michal!<br/><br/>
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{{#calendar: title=Glasgow |year=2011 | month=08}}
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{{#calendar: title=Glasgow |year=2011 | month=09}}
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<iframe width="560" height="315" src="http://www.youtube.com/embed/0sEjIqsmJxY" frameborder="2" allowfullscreen align=left></iframe>
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<br/><br/><ul>&nbsp;<u>This week:</u><br/>
 +
<ul>* Our new lab was set up</ul>
 +
<ul>* We created glycerol stocks of <i>E.coli</i> and <i>Pseudomonas</i> strains for future use</ul>
 +
<ul>* We had a meeting with <a href=http://www.gla.ac.uk:443/ibls/staff/staff.php?who=Ped|dQ>Dr Daniel Walker</a> who gave us some really useful information</ul>
 +
<ul>* We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them</ul> </ul>
 +
<br/><br/><br/><br/><br/><br/>
 +
 
 +
<h2>Week 2 Summary</h2>
 +
This week the video is presented by Ellin Hillert. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Ellin_Hillert>here</a> to find out more about Ellin!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/7wHpCv_hCOY" frameborder="2" allowfullscreen align=LEFT></iframe>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* We began the week with yet another transformation failure, resulting in our first successful transformants!</ul>
 +
<ul>* Our secret strain of <i>E.coli</i> arrived!</ul>
 +
<ul>* We each gave a presentation on different aspects of our project</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
<h2>Week 3 Summary</h2>
 +
This week the video is presented by Andrew Landels. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Andrew_Landels>here</a> to find out more about Andrew!<br /><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/MhnMF-s4DRo" frameborder="2" allowfullscreen align=LEFT></iframe>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* We now have successful transformations of all our biobricks!</ul>
 +
<ul>* Our secret strain of <i>E.coli</i> forms really nice biofilms!</ul>
 +
<ul>* We had our very first Wiki Whaaaaaam!</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
 
 +
<h2>Week 4 Summary</h2>
 +
This week the video is presented by Pietro Ridone.  Click <a href=https://2011.igem.org/Team:Glasgow/Team/Pietro_Ridone>here</a> to find out more about Pietro!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/BpCmEpKz-h4" frameborder="2" allowfullscreen align=LEFT></iframe></center>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* Work to gather data on biofilm growth and dispersal continues.</ul>
 +
<ul>* Primers for biobricks arrived</ul>
 +
<ul>* The guys have given us a sneak preview of the Norwich presentation</ul>
 +
<ul>* We have acquired our own Flouresence Microscope...</ul>
 +
</ul>
 +
<br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
 
 +
<h2>Week 5 Summary</h2>
 +
This week the video is presented by Chris Wood. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Chris_Wood>here</a> to find out more about Chris!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/rs6vdCWG8yU" frameborder="2" allowfullscreen align=left></iframe></center>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* The guys have returned from Norwich with some great insights</ul>
 +
<ul>* Everyone is excited to incorporate human practices into the project in a new way</ul>
 +
<ul>* We now have a plan for Modelling</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
<h2>Week 6 Summary</h2>
 +
This week the video is presented by Hannah Ralph. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Hannah_Ralph>here</a> to find out more about Hannah!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/6S6KtfYjfg8" frameborder="2" allowfullscreen align=left></iframe>
 +
 
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* We started ligating our constructs</ul>
 +
<ul>* PCR was carried out on our novel biobricks</ul>
 +
<ul>* Our secret <i>E.coli</i> strain was successfully transformed with RFP!</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
 
 +
<h2>Week 7 Summary</h2>
 +
This week the video is presented by Emma Campbell. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Emma_Campbell>here</a> to find out more about Emma!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/epBvEuJJtds" frameborder="2" allowfullscreen align=left></iframe>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* Edinburgh sent us some biobricks to work with</ul>
 +
<ul>* We realised our novel biobricks have illegal restriction sites, so ordered primers for Site Directed Mutagenesis</ul>
 +
<ul>* Our poster for the Open Day is looking great!</ul>
 +
<ul>* Amsterdam is booked - everyone is really excited!</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
<h2>Week 8 Summary</h2>
 +
This week the video is presented by Stewart O'Neill. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Stewart_O%27Neill>here</a/> to find out more about Stewart!<br/><br/>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/ZiJpsuw36nM" frameborder="2" allowfullscreen align=left></iframe>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* Work on our light responsive constructs and Edinburgh biobricks is continuing</ul>
 +
<ul>* An EnvZ negative strain of <i>E.coli</i> arrived from the Registry</ul>
 +
<ul>* Modelling is going really well</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/><br/>
 +
 
 +
 
 +
<h2>Week 9 Summary</h2>
 +
This week the video is presented by Scott Wood. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Scott_Wood>here</a> to find out more about Scott!<br/><br>
 +
 
 +
<iframe width="560" height="315" src="http://www.youtube.com/embed/ePeOcq4zEKY" frameborder="2" allowfullscreen align=left></iframe>
 +
<br/><br/>&nbsp;<ul><u>This week:</u><br/>
 +
<ul>* PCR and Site Directed Mutagenesis is continuing on our novel biobricks</ul>
 +
<ul>* Our work with the EnvZ negative cells is ongoing</ul>
 +
<ul>* Modelling looks great - we now have some really great graphs for our dispersal biobricks!</ul>
 +
</ul>
 +
<br/>
 +
<br/><br/><br/><br/><br/><br/><br/><br/>
 +
</html>

Latest revision as of 22:59, 20 September 2011

Video Logs

Want to know how life in the lab is going? Check out our weekly videos!

Week 1 Summary

This week the video is presented by Michal Przydacz. Click here to find out more about Michal!



     This week:
      * Our new lab was set up
      * We created glycerol stocks of E.coli and Pseudomonas strains for future use
      * We had a meeting with Dr Daniel Walker who gave us some really useful information
      * We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them






Week 2 Summary

This week the video is presented by Ellin Hillert. Click here to find out more about Ellin!



 
    This week:
      * We began the week with yet another transformation failure, resulting in our first successful transformants!
      * Our secret strain of E.coli arrived!
      * We each gave a presentation on different aspects of our project









Week 3 Summary

This week the video is presented by Andrew Landels. Click here to find out more about Andrew!



 
    This week:
      * We now have successful transformations of all our biobricks!
      * Our secret strain of E.coli forms really nice biofilms!
      * We had our very first Wiki Whaaaaaam!










Week 4 Summary

This week the video is presented by Pietro Ridone. Click here to find out more about Pietro!



 
    This week:
      * Work to gather data on biofilm growth and dispersal continues.
      * Primers for biobricks arrived
      * The guys have given us a sneak preview of the Norwich presentation
      * We have acquired our own Flouresence Microscope...








Week 5 Summary

This week the video is presented by Chris Wood. Click here to find out more about Chris!



 
    This week:
      * The guys have returned from Norwich with some great insights
      * Everyone is excited to incorporate human practices into the project in a new way
      * We now have a plan for Modelling









Week 6 Summary

This week the video is presented by Hannah Ralph. Click here to find out more about Hannah!



 
    This week:
      * We started ligating our constructs
      * PCR was carried out on our novel biobricks
      * Our secret E.coli strain was successfully transformed with RFP!










Week 7 Summary

This week the video is presented by Emma Campbell. Click here to find out more about Emma!



 
    This week:
      * Edinburgh sent us some biobricks to work with
      * We realised our novel biobricks have illegal restriction sites, so ordered primers for Site Directed Mutagenesis
      * Our poster for the Open Day is looking great!
      * Amsterdam is booked - everyone is really excited!









Week 8 Summary

This week the video is presented by Stewart O'Neill. Click here to find out more about Stewart!



 
    This week:
      * Work on our light responsive constructs and Edinburgh biobricks is continuing
      * An EnvZ negative strain of E.coli arrived from the Registry
      * Modelling is going really well










Week 9 Summary

This week the video is presented by Scott Wood. Click here to find out more about Scott!



 
    This week:
      * PCR and Site Directed Mutagenesis is continuing on our novel biobricks
      * Our work with the EnvZ negative cells is ongoing
      * Modelling looks great - we now have some really great graphs for our dispersal biobricks!









Retrieved from "http://2011.igem.org/Team:Glasgow/iGEM_Diary"