Team:UQ-Australia/Notebook/August

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|rowspan="2"|After still failing to produce any positive results from our cloning, we discovered that our parts had been incorrectly synthesized at this point. We sourced some genes from elsewhere and instead began focusing on other components of our system. We spent a lot of time this month finalizing other Outreach opportunities and doing work related to our Human Practices section, as well as developing the laboratory work.
|rowspan="2"|After still failing to produce any positive results from our cloning, we discovered that our parts had been incorrectly synthesized at this point. We sourced some genes from elsewhere and instead began focusing on other components of our system. We spent a lot of time this month finalizing other Outreach opportunities and doing work related to our Human Practices section, as well as developing the laboratory work.
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== '''<span style="color:#558822"> Summary of Lab Work </span>''' ==
== '''<span style="color:#558822"> Summary of Lab Work </span>''' ==
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We purified these pieces and digested them, but subsequent ligations into the plasmid backbone pSB1C3 failed.
We purified these pieces and digested them, but subsequent ligations into the plasmid backbone pSB1C3 failed.
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30 Aug 2011 - gel electrophoresis and purification of the above digestions - did not obtain right sized bands for GlnAp2, Plac/ara or glnG. pSb1C3, LacI and GFP were purified.
 
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31 Aug 2011 - re-transformation of LacI, GlnAp2 and Plac/ara from MrGene
 

Latest revision as of 00:46, 6 October 2011




After still failing to produce any positive results from our cloning, we discovered that our parts had been incorrectly synthesized at this point. We sourced some genes from elsewhere and instead began focusing on other components of our system. We spent a lot of time this month finalizing other Outreach opportunities and doing work related to our Human Practices section, as well as developing the laboratory work. UQ-Australia logo 2011.png

Summary of Lab Work

We repeated the digestion, ligation and transformations of a number of out parts before determining the reason behind why these weren't working.

At this point, we turned to using PCR to amplify some parts from plasmids we had been provided by Dr Alex Ninfa and Dr Susan Rowland, with gels showing bands of the correct sizes.

We purified these pieces and digested them, but subsequent ligations into the plasmid backbone pSB1C3 failed.

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