Team:UQ-Australia/Parts

From 2011.igem.org

(Difference between revisions)

Latest revision as of 03:58, 6 October 2011

The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.

Here we demonstrate a biological clock being standardised as a set of BioBrick parts.

Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.

We were not completely successful in our experimental endeavours towards making the BioBrick parts to be submitted to the registry, however we were able to add BioBrick sites to each part listed below. Therefore, except for Plac/ara we had all the parts ready to be cloned into the iGEM submission vector pSB1C3. For more information on the laboratory work please see the Project page.

Parts

	Plac/ara A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter		glnG Encodes for DNA-binding protein(NR1), the activity of which depends on its phosphorylation. Drives expression of genes under GlnAp2 promoter
	araC Encodes for a positive regulatory protein for L-arabinose utilization in E.coli		pBAD L-arabinose inducible promoter
	LacI DNA-binding transcriptional inhibitor for lac-operon		GlnAp2 Promoter driven by phosphorylated GlnG(NR1)

@@ Line 1: / Line 1: @@
 {{:Team:UQ-Australia/Template:Header}}
-{|align="justify"
+{|style="width:100%;" border="0" cellpadding="10" cellspacing="0"
-|The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.
+|- valign="top"
+|rowspan="2"|The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.
 Here we demonstrate a biological clock being standardised as a set of BioBrick parts.
-|[[File:IGEM basic Logo stylized.png|125x125px|link=https://2011.igem.org]]
+Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.
+We were not completely successful in our experimental endeavours towards making the BioBrick parts to be submitted to the registry, however we were able to add BioBrick sites to each part listed below. Therefore, except for Plac/ara we had all the parts ready to be cloned into the iGEM submission vector pSB1C3. For more information on the laboratory work please see the [https://2011.igem.org/Team:UQ-Australia/Project '''Project page'''].
+! [[File:UQ-Australia_logo_2011.png|125x125px|link=https://2011.igem.org/Team:UQ-Australia]]
 |-
-|
-Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.
-|[[File:UQ-Australia_logo_2011.png|125x125px|link=https://2011.igem.org/Team:UQ-Australia]]
 |
 |}
-<!--- The Mission, Experiments --->
+===<span style="color:#558822"> Parts </span>===
-===<span style="color:#558822"> Parts </span>===
+{| border="0" cellpadding="10" cellspacing="0" width="892"
-== '''<span style="color:#558822"> Students </span>''' ==
+|width="100"|[[File:MultiplePromoter image.png|100x100px|link=https://2011.igem.org/File:Promoter_image.png#filelinks]]
+|width="250"|'''<span style="color:#D4A017">Plac/ara</span>'''
+A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter
+|width="100"|[[File:Gene image.png|100x100px]]
-{| border="1" cellpadding="10" cellspacing="0" width="892"
+|width="250"|'''<span style="color:#D4A017">glnG</span>'''
-|width="125"|[[File:Aakash.jpg|125x125px|link=https://igem.org/User:Aakash]]
+Encodes for DNA-binding protein(NR1), the activity of which depends on its phosphorylation. Drives expression of genes under GlnAp2 promoter
-|width="321"|'''<span style="color:#D4A017">Plac/ara</span>'''
-Third year Bachelor of Biomedical Sciences majoring in Neurobiology and Developmental Science
-|width="125"|[[File:Akash.jpg|125x125px|link=https://igem.org/User:aakashboda]]
-|width="321"|'''<span style="color:#D4A017">glnG</span>'''
-Third year Bachelor of Biotechnology majoring in Bioinformatics
 |-
-|[[File:AnnBui.png|125x125px|link=https://2011.igem.org/User:Ann]]
+|[[File:Gene image.png|100x100px]]
 |'''<span style="color:#D4A017">araC</span>'''
-Third year Bachelor of Science majoring in Physics
+Encodes for a positive regulatory protein for L-arabinose utilization in E.coli
-|[[File:Rhianna.png|125x125px|link=https://2011.igem.org]]
+|[[File:PositivePromoter.png|100x100px]]
 |'''<span style="color:#D4A017">pBAD</span>'''
-Third year Bachelor of Biotechnology majoring in Molecular Biotechnology
+L-arabinose inducible promoter
 |-
-|[[Image:Hyun.jpg.png|125x125px|link=https://2011.igem.org/User:Rhianna]]
+|[[File:Gene image.png|100x100px]]
 |'''<span style="color:#D4A017">LacI</span>'''
-Third year Bachelor of Biomedical Sciences majoring in Neuroscience
+DNA-binding transcriptional inhibitor for lac-operon
-|[[Image:Josh.jpg|125x125px|link=https://2011.igem.org]]
+|[[File:PositivePromoter.png|100x100px]]
 |'''<span style="color:#D4A017">GlnAp2</span>'''
-Third year Bachelor of Biomedical Sciences majoring in Human Genetics and Biomedical Science
+Promoter driven by phosphorylated GlnG(NR1)
 |}
-New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
-<groupparts>iGEM010 UQ-Australia</groupparts>