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Copenhagen/6 July 2011
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=='''Wednesday'''== | =='''Wednesday'''== | ||
| - | + | Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday. | |
| + | |||
| + | |||
| + | ===Lab Work=== | ||
| + | |||
| + | * Digestion of our PCR products - see protocol [[Team:Copenhagen/Protocol#Mutations|Mutations]] | ||
| + | |||
| + | * Transformation of E.coli cells with the mutant plasmids - see protocol [[Team:Copenhagen/Protocol#Transformations|Transformations]] | ||
| + | |||
| + | * [[Team:Copenhagen/Protocol#PCR_Reaction|PCR]] on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick. | ||
| + | |||
| + | |||
| + | ===Other Work=== | ||
| + | |||
| + | *We have send out e-mails to danish biotech companies in hope to achieve economical support. | ||
| + | |||
| + | Back to [[Team:Copenhagen/Notebook|Notebook]] | ||
Latest revision as of 12:33, 7 July 2011
Wednesday
Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday.
Lab Work
- Digestion of our PCR products - see protocol Mutations
- Transformation of E.coli cells with the mutant plasmids - see protocol Transformations
- PCR on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick.
Other Work
- We have send out e-mails to danish biotech companies in hope to achieve economical support.
Back to Notebook
