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Team:TzuChiU Formosa/Notebook/photopaper
From 2011.igem.org
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| - | <font color="# | + | <font color="#228B22" size=6>Photopaper</font> |
| - | <br><br><font color="# | + | <br><br><font color="#000080" size=4>Meeting Minutes</font> |
<Hr Align="left" width="100%" size=2> | <Hr Align="left" width="100%" size=2> | ||
| - | + | <font color="#000000" size=3><b>2011.02.24</b></font> | |
| - | + | <br><font size=2><b>Discussion:</b></font> | |
| - | + | ||
| - | <br><font size= | + | |
*Team organization[[File:001.jpg|right|500px|caption]] | *Team organization[[File:001.jpg|right|500px|caption]] | ||
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<br><font color="#000000" size=3><b>2011.03.04</b></font> | <br><font color="#000000" size=3><b>2011.03.04</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
*Team advisory | *Team advisory | ||
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<br><font color="#000000" size=3><b>2011.03.14</b></font> | <br><font color="#000000" size=3><b>2011.03.14</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
*Task Allocation | *Task Allocation | ||
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<br><font color="#000000" size=3><b>2011.03.23</b></font> | <br><font color="#000000" size=3><b>2011.03.23</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
**Project : paperia | **Project : paperia | ||
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<br><font color="#000000" size=3><b>2011.03.24</b></font> | <br><font color="#000000" size=3><b>2011.03.24</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
* Exp. procedure: | * Exp. procedure: | ||
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<br><font color="#000000" size=3><b>2011.06.22</b></font> | <br><font color="#000000" size=3><b>2011.06.22</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
* Due to some unforseen reason, the team decided to change their project. | * Due to some unforseen reason, the team decided to change their project. | ||
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<br><font color="#000000" size=3><b>2011.07.01</b></font> | <br><font color="#000000" size=3><b>2011.07.01</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
* Freeze > grin > genome DNA isolation > Cloning = silk protein gene | * Freeze > grin > genome DNA isolation > Cloning = silk protein gene | ||
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<br><font color="#000000" size=3><b>2011.07.09</b></font> | <br><font color="#000000" size=3><b>2011.07.09</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
* the connections between 3 silk proteins : Fibl Fibh P25 | * the connections between 3 silk proteins : Fibl Fibh P25 | ||
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<br><font color="#000000" size=3><b>2011.07.15</b></font> | <br><font color="#000000" size=3><b>2011.07.15</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
* Due to limited resources and techniques required, the team decided to switch back to the previous project. Paper making ! | * Due to limited resources and techniques required, the team decided to switch back to the previous project. Paper making ! | ||
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<br><font color="#000000" size=3><b>2011.07.18</b></font> | <br><font color="#000000" size=3><b>2011.07.18</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
* Latest project : Photo paper | * Latest project : Photo paper | ||
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<br><font color="#000000" size=3><b>2011.07.23</b></font> | <br><font color="#000000" size=3><b>2011.07.23</b></font> | ||
| - | <br><font size= | + | <br><font size=2><b>Discussion:</b></font> |
* system modification to overcome the problems arises during preliminary round | * system modification to overcome the problems arises during preliminary round | ||
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Latest revision as of 02:56, 6 October 2011
Photopaper
Meeting Minutes
2011.02.24
Discussion:
- Team organization
- Brain storming
- paper made by bacteria with add-ons such as colors, fragrance, etc.
- "light up" the plants for replacing lamp posts.
2011.03.04
Discussion:
- Team advisory
- Brain storming
- Add-ons: colorful cellulose which produced by bacteria such as beta-carotene
- information exchange with iGEM 2009 Cambridge team
2011.03.14
Discussion:
- Task Allocation
- Brain storming
- Avatar - "light up" the plants by transfecting symbiotic bacteria which cloned into fluorescence gene
- Eco-friendly warmer - biotic thermal pad
2011.03.23
Discussion:
- Project : paperia
- Option 1 : Culture bacteria which has pigment gene
- Option 2 : Cellulose-producing bacteria secrete pigment into the medium
2011.03.24
Discussion:
- Exp. procedure:
- cloning of cellulose gene’s CDS
- the product should operate within E. coli.
2011.06.22
Discussion:
- Due to some unforseen reason, the team decided to change their project.
- New project: Biojenny
-economical and humane way to produce paper in large quantities.
-yeast to be our host
2011.07.01
Discussion:
- Freeze > grin > genome DNA isolation > Cloning = silk protein gene
2011.07.09
Discussion:
- the connections between 3 silk proteins : Fibl Fibh P25
- major proteins : H-chain, L-chain, P25
2011.07.15
Discussion:
- Due to limited resources and techniques required, the team decided to switch back to the previous project. Paper making !
- However it would be modified to be more innovative and creative.
2011.07.18
Discussion:
- Latest project : Photo paper
- cyanobacteria is designed as the host, cellulose made up of the glucose produced by cyanobacteria could be one of the main attraction of the project.
2011.07.23
Discussion:
- system modification to overcome the problems arises during preliminary round
- Biobricks from Tokyo 2010 team will be utilized
- regulator promoter in order to regulate the secretion of cellulose to solve the aggregation of the bacteria
2011.09.15
Genome miniprep
Gluconacetobacter hansenii
2011.09.18
Gel/PCR DNA extraction
Gluconacetobacter hansenii
Protocols
