Team:ZJU-China/Protocol

From 2011.igem.org

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<p style="margin-left:10px;">This group...........</p>
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<h4 class="current">September</h4>  
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<h4 >September</h4>  
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<div class="pane" >  
                 <a  href="https://2011.igem.org/Team:ZJU-China/Notebook/September">In this month we......<br/>>>Click to see</a>
                 <a  href="https://2011.igem.org/Team:ZJU-China/Notebook/September">In this month we......<br/>>>Click to see</a>
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<h4>Protocol</h4>
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<h4 class="current">Protocol</h4>
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         <div class="pane"><a href="https://2011.igem.org/Team:ZJU-China/Protocol">>>Click to see our lab protocol about biofilm formation</a></div>
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         <div class="pane" style="display:block;"><a href="https://2011.igem.org/Team:ZJU-China/Protocol">>>Click to see our lab protocol about biofilm formation</a></div>
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<h3>Week1</h3><hr/>  
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<h3>Biofilm Formation</h3>
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<table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">  
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<p>Biofilm Formation Protocol with common lab equipment:</p>
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  <tr><td width="76">Day</td><td width="349">Note</td></tr>  
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<a style="display:block;margin:20px;" href="http://ung.igem.org/wiki/images/b/b1/Biofilm_protocol.pdf"><img src="http://ung.igem.org/wiki/images/1/1b/Adobe_Reader_Logo.jpg" width="40" height="40"/>download PDF version</a>
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    <td id="sheetleft">Jul.4th Monday</td>  
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    <td width="128">• Aerobic cultivation of DH5α</td>
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<h3>Basic Protocols</h3><hr/>
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<p>&nbsp;</p>
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    <td width="196">• Preparation of apparatus for the formation of biofilm</td>  
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<p>AxyPrep™ Plasmid Miniprep Kit</p>
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<a style="display:block;margin:20px;" href="http://static0.jadedpixel.com/s/files/1/0001/7488/files/axyprep_plasmid_mini_protocol.pdf"><img src="http://ung.igem.org/wiki/images/1/1b/Adobe_Reader_Logo.jpg" width="40" height="40"/>download PDF version</a>
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    <td id="sheetleft">Jul 5th Tuesday
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<p>AxyPrep™ PCR Cleanup Kit</p>
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    <td id="sheetleft">Jul.6th
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Wednesday
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href="http://static3.jadedpixel.com/s/files/1/0001/7488/files/axyprep_pcr_cleanup_protocol.pdf"><img src="http://ung.igem.org/wiki/images/1/1b/Adobe_Reader_Logo.jpg" width="40" height="40"/>download PDF version</a>
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    <td width="215">•Receiving  primers ordered previously</td>  
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<p>TaKaRa Agarose Gel DNA Purification Kit Ver.2.0</p>
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href="http://www.takara.com.cn/explain/D/DV805A.pdf"><img src="http://ung.igem.org/wiki/images/1/1b/Adobe_Reader_Logo.jpg" width="40" height="40"/>download PDF version</a>
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    <td id="sheetleft">Jul.7th
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Thursday
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<p>Chemically competent E.coli preparation.</p>
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    <td width="200">• Preparation of the aliquot of the primers</td>
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href="http://www.openwetware.org/wiki/Preparing_chemically_competent_cells">See official protocol</a>
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    <td id="sheetleft">Jul.8th
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Friday
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<p>Transformation</p>
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href="http://www.openwetware.org/wiki/Transforming_chemically_competent_cells_%28Inoue%29">See official protocol</a>
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        <td width="155">• Preparation of culture plates for the transformations</td>
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    <td id="sheetleft">Jul.9th
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<p>Colony PCR:</p>
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Saturday
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    <td><table width="313" border="0" cellspacing="0" cellpadding="1">  
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href="http://www.openwetware.org/wiki/Endy:Colony_PCR">See official protocol</a>
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    <td width="127">• Preparation of culture plates for the transformations</td>  
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<div class="block" id="mondy">
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    <td width="182">• protocols of transformation</td>
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<p>Agarose gel electrophoresis</p><hr/>
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<a style="display:block;margin:20px;"
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href="http://www.openwetware.org/wiki/Agarose_gel_electrophoresis">See official protocol</a>
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    <td id="sheetleft">Jul.10th
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Sunday
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        <td>• Several colonies were picked up and cultivated in  5mL LB medium.
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•Cryosectioning of biofilm
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Latest revision as of 03:01, 6 October 2011

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Protocol

Biofilm Formation

Biofilm Formation Protocol with common lab equipment:

download PDF version

Basic Protocols

 

AxyPrep™ Plasmid Miniprep Kit

download PDF version

AxyPrep™ PCR Cleanup Kit

download PDF version

TaKaRa Agarose Gel DNA Purification Kit Ver.2.0

download PDF version

Chemically competent E.coli preparation.

See official protocol

Transformation

See official protocol

Colony PCR:

See official protocol

Agarose gel electrophoresis

See official protocol

Retrieved from "http://2011.igem.org/Team:ZJU-China/Protocol"