Team:IIT Madras/Notebook

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<H1><u>Notebook</u></H1>
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<h2>Week 1</h2>
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<li>Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin
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<li>Literature survey on expression of Proteorhodopsin in E.coli<br><br>
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<h2>Week 2</h2>
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<li>Minimal media characterization of DH5-alpha
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<li>Modelling survival of PR transformed E.coli cells v/s wild type E.coli
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<li>Literature survey on increase in recombinant protein expression in PR transformed cells<br><br>
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<h2>Week 3</h2>
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<li>Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea.
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<li>Designing the incubator for experimental studies with Proteorhodopsin
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<li>Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide<br><br>
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<h2>Week 4</h2>
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<li>Standardizing the protocol for digestion of pKJ900
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<li>Designing RFP generator for promoter studies<br><br>
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<h2>Week5</h2>
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<li>Generating the PcstA_RFP Generator
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<li>Synthesizing the T7_RFP Generator<br><br>
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<h2>Week6</h2>
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<li>Characterizing PcstA_RFP Generator
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<li>Designing the promoters for Proteorhodopsin gene
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<li>Codon optimization of Proteorhodopsin gene<br><br>
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<h2>Week7</h2>
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<li>Designing the OmpR Promoter_RFP Generator
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<li>Designing the PcstA_Luciferase Generator
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<li>Digestion of Promoters<br><br>
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<h2>Week8</h2>
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<li>Transformation of Promoters
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<li>Standardizing the protocols for transformation of proteorhodopsin
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<li>Designing the Proteorhodopsin Generator<br><br>
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<h2>Week9</h2>
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<li>Preparation for Presentation at IITM Research Expo
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<li>Lecture preparation on synthetic biology
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<li>Characterizing growth behaviour of Proteorhodopsin transformed cells on various minimal media under light/dark conditions.<br><br>
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<h2>Week10</h2>
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<li>Expression studies of the recombinant protein in proteorhodopsin transformed E.coli
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<li>Presentation at IITM Research Expo<br><br>
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<h2>Week11</h2>
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<h1 style="color:white;">IIT Madras</h1>
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<h3 style="color:white;">And then the E.coli said, "Let there be light"</h3></div>
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<b class="group">Cloning</p><br/>
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<div id="part1_title" class="part_title">Proteorhodopsin</div>
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<b>Expression Studies</p>
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<li><a href="https://2011.igem.org/Team:IIT_Madras">Home</a></li>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Team">Team</a></li>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Project">Project</a></li>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Parts">Parts</a></li>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Modeling">Modeling</a></li>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook">Notebook</a></li>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Safety">Safety</a></li>
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<li><a href="https://2011.igem.org/Team:IIT_Madras/Attributions">Attributions</a></li>
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Latest revision as of 21:56, 28 October 2011

bar iGEM 2011 - Home Page Indian Institute of Technology - Madras




Contents

Notebook

Week 1

  • Metabolic simulation and modelling of increase in proton flux on addition of Proteorhodopsin
  • Literature survey on expression of Proteorhodopsin in E.coli

    Week 2

  • Minimal media characterization of DH5-alpha
  • Modelling survival of PR transformed E.coli cells v/s wild type E.coli
  • Literature survey on increase in recombinant protein expression in PR transformed cells

    Week 3

  • Obtained Proteorhodopsin gene and Beta-Dioxygenase in PKJ-900 from Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea.
  • Designing the incubator for experimental studies with Proteorhodopsin
  • Experimental analysis of growth rates of PR transformed E.coli and DH5-alpha in presence of ETC-Inhibitor Azide

    Week 4

  • Standardizing the protocol for digestion of pKJ900
  • Designing RFP generator for promoter studies

    Week5

  • Generating the PcstA_RFP Generator
  • Synthesizing the T7_RFP Generator

    Week6

  • Characterizing PcstA_RFP Generator
  • Designing the promoters for Proteorhodopsin gene
  • Codon optimization of Proteorhodopsin gene

    Week7

  • Designing the OmpR Promoter_RFP Generator
  • Designing the PcstA_Luciferase Generator
  • Digestion of Promoters

    Week8

  • Transformation of Promoters
  • Standardizing the protocols for transformation of proteorhodopsin
  • Designing the Proteorhodopsin Generator

    Week9

  • Preparation for Presentation at IITM Research Expo
  • Lecture preparation on synthetic biology
  • Characterizing growth behaviour of Proteorhodopsin transformed cells on various minimal media under light/dark conditions.

    Week10

  • Expression studies of the recombinant protein in proteorhodopsin transformed E.coli
  • Presentation at IITM Research Expo

    Week11

  • Wiki designing


    • Retrieved from "http://2011.igem.org/Team:IIT_Madras/Notebook"