Team:IIT Madras/Notebook/Protocols/Preperation of antibiotics stock

From 2011.igem.org

(Difference between revisions)
(Created page with "{{:Team:IIT_Madras/Template:header}} <html> <head> <style> #main_content{ width:100%; height:100%; min-height:500px; background:none; position:relative; top:-69px; } #header{ wi...")
 
(2 intermediate revisions not shown)
Line 51: Line 51:
#content_menu ul li:hover{background:#ec9f09;}
#content_menu ul li:hover{background:#ec9f09;}
#content_menu ul li a{text-decoration:none; color:white; }
#content_menu ul li a{text-decoration:none; color:white; }
 +
 +
table{background:none; color:white;}
</style>
</style>
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.6.2/jquery.min.js"/></script>
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.6.2/jquery.min.js"/></script>
Line 68: Line 70:
<div id="content_body">
<div id="content_body">
<div id="content_text">
<div id="content_text">
-
<h1>Protocols</h1><br/>
+
<h1>Preperation of antibiotics stock</h1><br/>
-
<ul>
+
<table><tr><td>Antibiotic</td><td>Conc. (1000x)</td><td>Solvent</td></tr>
-
<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Preperation_of_competent_cells">Preperation of competent DH5alpha cells</a></li>  
+
<tr><td>Ampicillin</td><td>100 mg/ml</td><td>Water</td></tr>
-
<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Miniprep_using_kits">Miniprep using kit</a></li>  
+
<tr><td>Chloramphenicol</td><td>34 mg/ml</td><td>Ethanol</td></tr>
-
<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Gel_elusion">Gel Elusion</a></li>
+
<tr><td>Kanamycin</td><td>50 mg/ml</td><td>Water</td></tr>
-
<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Transformation">Transformation</a></li>
+
</table>
-
<li><a href="https://2011.igem.org/Team:IIT_Madras/Notebook/Protocols/Miniprep_using_alkaline_lysis_buffers">Miniprep using alkaline lysis buffers</a></li>
+
<p><b>Filter sterilize them</b><br/><br/>
-
</ul>
+
Add 1 ul for each ml of media (Applicable for all the above antibiotics).</p>
</div>
</div>
<div id="content_menu">
<div id="content_menu">

Latest revision as of 10:42, 2 October 2011

bar iGEM 2011 - Home Page Indian Institute of Technology - Madras

Preperation of antibiotics stock


AntibioticConc. (1000x)Solvent
Ampicillin100 mg/mlWater
Chloramphenicol34 mg/mlEthanol
Kanamycin50 mg/mlWater

Filter sterilize them

Add 1 ul for each ml of media (Applicable for all the above antibiotics).